1987
DOI: 10.1021/bk-1987-0352.ch001
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An Overview of Size Exclusion Chromatography for Polymers and Coatings

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Cited by 11 publications
(5 citation statements)
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“…Preparative size-exclusion chromatography (SEC) and gel permeation chromatography (GPC) have generally been used in research on humic substances as fractionation techniques rather than purification methods. Under ideal separation conditions, i.e., mobile phases with well-buffered pH values, ionic strengths of >0.02, and no hydrophobic solute−stationary phase interactions, , solutes with the largest sizes migrate through the column at the greatest rate, followed by solutes with progressively decreasing sizes, with no solutes eluting after the permeation limit (i.e., the total permeation volume of the column). Only a few studies have used SEC to analyze marine and estuarine DOM. , Huber and Frimmel (1994, 1996), , using on-line TOC detection, observed that the retention time of DOM solutes (either whole seawater or HMW isolates) is a function of both DOM molecular size and structural subunits.…”
Section: 51 Desalting By Size-exclusion Chromatographymentioning
confidence: 99%
“…Preparative size-exclusion chromatography (SEC) and gel permeation chromatography (GPC) have generally been used in research on humic substances as fractionation techniques rather than purification methods. Under ideal separation conditions, i.e., mobile phases with well-buffered pH values, ionic strengths of >0.02, and no hydrophobic solute−stationary phase interactions, , solutes with the largest sizes migrate through the column at the greatest rate, followed by solutes with progressively decreasing sizes, with no solutes eluting after the permeation limit (i.e., the total permeation volume of the column). Only a few studies have used SEC to analyze marine and estuarine DOM. , Huber and Frimmel (1994, 1996), , using on-line TOC detection, observed that the retention time of DOM solutes (either whole seawater or HMW isolates) is a function of both DOM molecular size and structural subunits.…”
Section: 51 Desalting By Size-exclusion Chromatographymentioning
confidence: 99%
“…The ASTM Standard Method D3536 [91,92] includes the ''Evaluation of Axial Dispersion'' to check SEC operation for peak skewing. The appropriate equations follow:…”
Section: Peak Skewingmentioning
confidence: 99%
“…This is unlikely for quadruplexes because of their exceptional stability. Third, protein samples can experience associative and repulsive interactions with column packing material 53,54 . Some negatively charged species may experience exclusion by repulsive interaction with the column matrix.…”
mentioning
confidence: 99%