An Overview of Size Exclusion Chromatography for Polymers and Coatings

Kuo, Cheng-Yih; Provder, Theodore

doi:10.1021/bk-1987-0352.ch001

Cited by 11 publications

(5 citation statements)

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“…Preparative size-exclusion chromatography (SEC) and gel permeation chromatography (GPC) have generally been used in research on humic substances as fractionation techniques rather than purification methods. Under ideal separation conditions, i.e., mobile phases with well-buffered pH values, ionic strengths of >0.02, and no hydrophobic solute−stationary phase interactions, , solutes with the largest sizes migrate through the column at the greatest rate, followed by solutes with progressively decreasing sizes, with no solutes eluting after the permeation limit (i.e., the total permeation volume of the column). Only a few studies have used SEC to analyze marine and estuarine DOM. ,− Huber and Frimmel (1994, 1996), , using on-line TOC detection, observed that the retention time of DOM solutes (either whole seawater or HMW isolates) is a function of both DOM molecular size and structural subunits.…”

Section: 51 Desalting By Size-exclusion Chromatographymentioning

confidence: 99%

Advanced Instrumental Approaches for Characterization of Marine Dissolved Organic Matter: Extraction Techniques, Mass Spectrometry, and Nuclear Magnetic Resonance Spectroscopy

et al. 2007

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Cluster (COSMIC). He is the author of more than 250 peer-reviewed articles that report on numerous studies in the area of environmental chemistry and geochemistry, specifically emphasizing the origin, structure, and chemical transformations of plant-derived biopolymers in soils, peats, marine sediments, and oceanic waters. Hatcher relies on modern analytical methods that include 1-, 2-, and 3D liquids NMR as well as solids NMR and electrospray ionization with ultra-high-resolution mass spectrometry.

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Section: 51 Desalting By Size-exclusion Chromatographymentioning

confidence: 99%

Advanced Instrumental Approaches for Characterization of Marine Dissolved Organic Matter: Extraction Techniques, Mass Spectrometry, and Nuclear Magnetic Resonance Spectroscopy

et al. 2007

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“…The ASTM Standard Method D3536 [91,92] includes the ''Evaluation of Axial Dispersion'' to check SEC operation for peak skewing. The appropriate equations follow:…”

Section: Peak Skewingmentioning

confidence: 99%

Size-exclusion chromatography—a review of calibration methodologies

Kostanski

Keller

Hamielec

2004

Journal of Biochemical and Biophysical Methods

165

134

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“…This is unlikely for quadruplexes because of their exceptional stability. Third, protein samples can experience associative and repulsive interactions with column packing material 53,54 . Some negatively charged species may experience exclusion by repulsive interaction with the column matrix.…”

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confidence: 99%

Polymorphism and resolution of oncogene promoter quadruplex-forming sequences

Miller

Dean

et al. 2011

Org. Biomol. Chem.

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We report the separation of several quadruplex species formed by ten promoter sequences by Size Exclusion Chromatography (SEC). Modification at the 5’ or 3’ ends or in loop regions of quadruplex forming sequences has become the standard technique for dealing with quadruplex polymorphism. However, conformations produced employing this method or by other means of artificially shifting the equilibrium may not represent the species that are present in vivo. This method enables an unperturbed view of the structural polymorphism inherent to quadruplex formation. Separation via SEC facilitates studies on quadruplex structure and biophysical properties without the need for sequence modification.

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An Overview of Size Exclusion Chromatography for Polymers and Coatings

Cited by 11 publications

References 0 publications

Advanced Instrumental Approaches for Characterization of Marine Dissolved Organic Matter: Extraction Techniques, Mass Spectrometry, and Nuclear Magnetic Resonance Spectroscopy

Advanced Instrumental Approaches for Characterization of Marine Dissolved Organic Matter: Extraction Techniques, Mass Spectrometry, and Nuclear Magnetic Resonance Spectroscopy

Size-exclusion chromatography—a review of calibration methodologies

Polymorphism and resolution of oncogene promoter quadruplex-forming sequences

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