An Overview of Livestock-Associated MRSA in Agriculture

In this study we investigated the kinetics of colonization, the host susceptibility and transmissibility of methicillin-resistant Staphylococcus aureus (MRSA) after nasal treatment of pigs with three different MRSA strains of distinctive clonal lineages (sequence type 398 [ST398], ST8, and ST9), and origin in weaning piglets. The colonization dose of 5.0 ؋ 10 8 CFU/animal was determined in preliminary animal studies. A total of 57 piglets were randomly divided into four test groups and one control group. Each of three test groups was inoculated intranasally with either MRSA ST8, MRSA ST9, or MRSA ST398. The fourth group was a mixture of animals inoculated with MRSA ST398 and noninoculated "sentinel" animals. Clinical signs, the nasal, conjunctival, and skin colonization of MRSA, fecal excretion, and organ distribution of MRSA, as well as different environmental samples were examined. After nasal inoculation with MRSA piglets of all four test groups showed no clinical signs of an MRSA infection. MRSA was present on the nasal mucosa, skin, and conjunctiva in all four test groups, including sentinel animals. Likewise, fecal excretion and internal colonization of MRSA ST8, ST9, and ST398 could be shown in each group. However, fecal excretion and the colonization rate of the nasal mucosa with MRSA ST9 were significantly lower in the first days after infection than in test groups infected with ST8 and ST398. The results of this study suggest differences in colonization potential of the different MRSA types in pigs. Furthermore, colonization of lymph nodes (e.g., the ileocecal lymph node) with MRSA of the clonal lineage ST398 was demonstrated.

show abstract

“…MRSA has been previously detected in air samples (15,33,38). In the present study, air samples were MRSA positive on only day 1 p.i.…”

Section: Discussionsupporting

confidence: 50%

Colonization Kinetics of Different Methicillin-Resistant Staphylococcus aureus Sequence Types in Pigs and Host Susceptibilities

Szabó

Beck²,

Friese³

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…number of other studies have reported a prevalence of MRSA in RP products ranging from as low as 2 to 45% (de Boer et al 2009;Harper et al 2010;Beneke et al 2011;Buyukcangaz et al 2013;Lassok and Tenhagen 2013). The presence of MRSA in RP could be due to survival of strains resistant to the temperatures during scalding, singeing, hot wash, pasteurization and refrigeration, or to cross-contamination from other products, environments (i.e.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of methicillin-resistant Staphylococcus aureus in Canadian commercial pork processing plants

et al. 2016

View full text Add to dashboard Cite

The highest prevalence of MRSA was found in the nasal cavity of incoming pigs in three commercial pig slaughter and pork processing plants. A reduction in MRSA prevalence occurred along the processing chain, and pork products from these plants showed significantly lower MRSA than the initial steps of slaughter and processing, suggesting a reduction in MRSA during the slaughter process with minimal cross-contamination.

show abstract

“…They collected a smaller air volume (100 liters versus Ͼ300 liters in our study) and used impaction instead of filtration and impingement for sampling. Impaction has been successfully used for MRSA detection in pig barns before (30), but its specific sensitivity for MRSA has not been compared to the sensitivities of the two sampling methods used in our study. In contrast to the results of a previous study carried out on pig farms (19), there was no significant difference between the results for the two air sampling methods in this study.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Turkey and Broiler Barns and Contamination of Air and Soil Surfaces in Their Vicinity

Friese¹,

Schulz

Zimmermann³

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

The emission of microorganisms, especially resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), from poultry farms is of public interest, and its occurrence and relevance are controversially discussed. So far, there are limited data on this issue. In this study, we investigated the occurrence of livestock-associated (LA)-MRSA inside and outside previously tested MRSA-positive poultry barns in Germany. In total, five turkey and two broiler fattening farms were investigated four and three times, respectively. In a longitudinal study during one fattening period, samples were collected from animals, the animals' environment inside the barn, including the air, and the barns' surroundings, such as ambient air and boot swabs of ground surfaces at different distances from the barn. Moreover, a cross-sectional study was carried out once inside the barns on five turkey and four broiler farms during the last third of the fatting period. In the cross-sectional study, LA-MRSA was detected in the air of most barns (7 of 9, 77.8%), as well as in many samples originating from animals, with detections levels of 50 to 54% in broiler and 62 to 77% in turkey farms. In the longitudinal study, LA-MRSA was found in the ambient air outside two turkey barns and on the ground surface on the downwind side of many (44.4%) turkey and broiler farms. The same spa types of isolates were observed inside and outside the barns. Transmission of MRSA within poultry farms, as well as emission via the airborne route, seems to be possible.

show abstract

An Overview of Livestock-Associated MRSA in Agriculture

Cited by 21 publications

References 16 publications

Colonization Kinetics of Different Methicillin-Resistant Staphylococcus aureus Sequence Types in Pigs and Host Susceptibilities

Colonization Kinetics of Different Methicillin-Resistant Staphylococcus aureus Sequence Types in Pigs and Host Susceptibilities

Prevalence of methicillin-resistant Staphylococcus aureus in Canadian commercial pork processing plants

Occurrence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Turkey and Broiler Barns and Contamination of Air and Soil Surfaces in Their Vicinity

Contact Info

Product

Resources

About