Prevalence of methicillin-resistant <i>Staphylococcus aureus</i>
 in Canadian commercial pork processing plants

Narváez‐Bravo, Claudia; Toufeer, Mehdi; Weese, Scott; Diarra, Moussa S.; Deckert, Anne E.; Reid‐Smith, Richard J.; Aslam, Mueen

doi:10.1111/jam.13024

Cited by 36 publications

(22 citation statements)

References 51 publications

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“…In fact, the prevalence of MRSA varies greatly by geographical location in retail meats. For example, MRSA was present in 1.9% of 3,520 retail meats in the USA (Ge et al, 2017 ), 0.5% (13/2,810) in Korea (Kim et al, 2015 ), 1.6% (5/318) in Spain, 13% in Denmark (19/145) (Tang et al, 2017 ), 11.9% in the Netherlands (264/2,217), and 24.8% in Canada (655/2,640) (Narvaez et al, 2016 ). Sample size, geographic area and collection period may responsible for the differences observed (Ge et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Staphylococcus aureus Isolated From Retail Meat and Meat Products in China: Incidence, Antibiotic Resistance and Genetic Diversity

Wu¹,

Huang²,

Wu³

et al. 2018

Front. Microbiol.

149

118

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This study was to estimate the prevalence and characteristics of Staphylococcus aureus from 1,850 retail meat and meat products in China during July 2011 to June 2016. The samples were collected covering most provincial capitals in China, including 604 raw meat, 601 quick-frozen meat, and 645 ready-to-eat meat. Using the qualitative and quantitative methods, all 39 cities had S. aureus-positive samples, and S. aureus was detected in 35.0% (647/1,850) of the samples. The levels of S. aureus in retail meat showed that the MPN value of the majority of the positive samples ranged from 0.3 to 100 MPN/g. Twenty-four antibiotics were used to test all 868 S. aureus isolates for antibiotic susceptibility. Only 11 isolates (1.26%) were susceptible to all antibiotics, whereas most isolates (821/868, 94.6%) showed resistance or intermediary resistance to more than three or more antibiotics. Of these strains, 104 (12.0%) were resistant to more than 10 antibiotics. However, the most frequent resistance was observed to ampicillin (85.4%), followed by penicillin (84.6%), erythromycin (52.7%), tetracycline (49.3%), kanamycin (45.3%), telithromycin (30.1%), clindamycin (29.6%), streptomycin (21.1%), norfloxacin (20.4%), gentamicin (19.4%), fusidic acid (18.4%), ciprofloxacin (16.9%), chloramphenicol (13.1%), amoxycillin/clavulanic acid (11.0%), and others (<10%). 7.4% of isolates (62/868) were confirmed as methicillin-resistance S. aureus (MRSA). By molecular typing analysis, there were 164 spa types and 111 STs were identified, including 15 novel spa types and 65 newly STs by multilocus sequence typing (MLST) and spa typing. Despite the wide genetic diversity observed among the 868 isolates, a great proportion of the population belonged to finite number of major clones: ST1-t127 (93/868, 10.7%) and ST7-t091 (92/868, 10.6%), ST5-t002 (42/868, 4.8%), ST398-t034 (40/868, 4.6%), ST188-t034 (38/868, 4.4%), ST59-t437 (30/868, 3.5%), ST6-t701 (29/868, 3.3%), and ST9-t899 (27/868, 3.1%) in China. This study reflects S. aureus was readily detected in Chinese retail meat and meat products but the level were not very excessive. In this study, the high antibiotic resistance is alarming and raising public health concern. In additions, most of molecular types of isolates have been linked to human infections around the world, indicating that these types of S. aureus in China have a theoretical pathogenic potential.

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Section: Discussionmentioning

confidence: 99%

Staphylococcus aureus Isolated From Retail Meat and Meat Products in China: Incidence, Antibiotic Resistance and Genetic Diversity

Wu¹,

Huang²,

Wu³

et al. 2018

Front. Microbiol.

149

118

View full text Add to dashboard Cite

show abstract

“…hardware factory or biscuit factory) in each city were sampled to enroll about 200 control workers with no occupational livestock exposure. After obtaining informed consent, two nasal swabs were taken from each participant and a face-to-face questionnaire was administered to collect influencing factors of S. aureus carriage, including sex, age (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40), and 41-60 years), education (elementary school, junior high school, senior high school, and above), personal monthly income (4¥1000, ¥1001-2000, ¥2001-3000, or 5¥3001), occupational pig exposure (yes or no), antimicrobial use in the last month (yes or no), and visit to medical facilities in the last month (yes or no).…”

Section: Study Design and Populationmentioning

confidence: 99%

Phenotypic and molecular characteristics ofStaphylococcus aureusand methicillin-resistantStaphylococcus aureusin slaughterhouse pig-related workers and control workers in Guangdong Province, China

Wang

et al. 2017

Epidemiol. Infect.

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Pig farmers and veterinarians have high prevalence of methicillin-resistant Staphylococcus aureus (MRSA) due to the occupational livestock exposure, while few reported this association on slaughterhouse workers. We conducted this cross-sectional study to explore the phenotypic and molecular characteristics of S. aureus and MRSA in slaughterhouse pig-related workers and control workers in Guangdong Province, China. Participants were interviewed and provided two nasal swabs. Swabs were tested for S. aureus, and isolates were further tested for antimicrobial susceptibility, virulence genes and multi-locus sequence typing. Compared with control workers, pig-related workers have significantly higher prevalence of MRSA carriage (adjusted odd ratio (aOR) 3·70, 95% CI 1·63-8·40). The proportions of MRSA resistant to clindamycin, erythromycin, tetracycline or chloromycetin were significantly higher in pig-related workers than in control workers. The predominant phenotypes of S. aureus were resistant to penicillin, clindamycin, erythromycin and tetracycline. Three MRSA CC9 isolates with livestock-associated characteristics (resistance to tetracycline and absence of immune evasion cluster (IEC) genes) were detected in pig-related workers but not in control workers. For human-associated CCs (CC7, CC59, CC6, and CC188), there was no significant difference in IEC profile or antimicrobial resistance between the groups. These findings reveal that there may be a potential risk for livestock-to-human transmission of LA-MRSA and human-to-human transmission of human-associated MRSA.

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“…; EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control) ; Narvaez‐Bravo et al . ). Nevertheless, the importance of food‐borne contamination in human MRSA infection or colonization remains unclear.…”

Section: Introductionmentioning

confidence: 97%

“…The identification of LA-MRSA in food-producing animals has prompted concerns about the possible contamination of food, and studies conducted in Europe, Asia and North America show that MRSA can be recovered from retail meat (Kitai et al 2005;de Boer et al 2009;Bhargava et al 2011;Weese et al 2010;Feßler et al 2011;O'Brien et al 2012;Vestergaard et al 2012; EFSA (European Food Safety Authority) and ECDC (European Centre for Disease Prevention and Control) 2015; Narvaez-Bravo et al 2016). Nevertheless, the importance of food-borne contamination in human MRSA infection or colonization remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Detection and molecular characterization of Livestock-Associated MRSA in raw meat on retail sale in North West England

Pichon

Wilkinson

Doumith

et al. 2017

Lett Appl Microbiol

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Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a public health concern worldwide, but has only been reported sporadically in the UK. In the largest UK study to date, samples of raw meat at retail sale were examined for both the presence and levels of MRSA. We report the first isolations of CC398 LA-MRSA from poultry meat in the UK including representatives of a particular sub-clade associated with cases of human infection/colonization in Europe. Although levels were low (<20 CFU per g), the contamination rate was higher than previous UK studies. Moreover, whole-genome sequencing revealed multiple independent introductions of LA-MRSA into the UK food chain.

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Prevalence of methicillin-resistant Staphylococcus aureus in Canadian commercial pork processing plants

Cited by 36 publications

References 51 publications

Staphylococcus aureus Isolated From Retail Meat and Meat Products in China: Incidence, Antibiotic Resistance and Genetic Diversity

Staphylococcus aureus Isolated From Retail Meat and Meat Products in China: Incidence, Antibiotic Resistance and Genetic Diversity

Phenotypic and molecular characteristics ofStaphylococcus aureusand methicillin-resistantStaphylococcus aureusin slaughterhouse pig-related workers and control workers in Guangdong Province, China

Detection and molecular characterization of Livestock-Associated MRSA in raw meat on retail sale in North West England

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