An overview of enzyme-based biosensors for environmental monitoring

Sarkar, Angana; Sarkar, Kuntal Deb; Vijayakumar, A.; Dutta, Kasturi

doi:10.1016/b978-0-12-814679-8.00015-7

Cited by 17 publications

(20 citation statements)

References 96 publications

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“…In the last decades, the concerns involving environmental pollution have intensified due to the constant growth of contaminants and pollutants harmful to the environment [334]. There is a clear need for developing new methods for monitoring the levels of pollutants in the environment, such as heavy metals and pesticides [335,336]. Traditional analytical techniques, such as chromatographic methods, are challenging to incorporate because they require costly reagents, time-consuming sample pre-treatments, and expensive equipment, apart from not being efficient in in situ measurements, as in the case of accidental releases of pesticides or acute poisoning [21,337,338].…”

Section: Environmental Applicationsmentioning

confidence: 99%

“…In this context, enzyme-based biosensors appear as a potential alternative, as they have several advantages over other traditional tools, by being economical, user-friendly, highly sensitive, accurate, and large-scale enabled [335,340,341]. Currently, the primary water and soil pollutants are heavy metals, such as aluminum (Al), cadmium (Cd), copper (Cu), lead (Pb), chromium (Cr), mercury (Hg), and zinc (Zn) [342,343]; and pesticides, such as atrazine, endosulfan sulfate, diethyl atrazine, chlorpyrifos, etc.…”

Section: Environmental Applicationsmentioning

confidence: 99%

“…Several enzymes, such as urease [347][348][349], horseradish peroxidase [343,350,351], glucose oxidase [287,352], invertase [353,354], and dehydrogenase [355][356][357] can be highly efficient in metal identification analyses, based either on the activation or on the inhibition of the specific enzyme. Heavy metals generally act as cofactors of metalloproteins to form an essential part of the structure that is required for enzyme activation [335,342]. Enzyme inhibition, on the other hand, occurs in the parts of the enzyme where the interaction between heavy metals and thiol or methylthiol groups of amino acids takes place [337,358].…”

Section: Heavy Metal Sensingmentioning

confidence: 99%

“…OPH has emerged as a promising alternative for detecting organophosphorus compounds, owing to its wide range of compatible substrates and its variety of possible bonds, such as P-F, P-O, P-S, and P-CN [375]. It is also highly specific for the detoxification of the PdF bond (G agents) of organophosphates such as sarin; however, OPH is ineffective for other classes of organophosphates such as PdO, PdS, and PdCN [335]. In another work, Du et al designed a nanomaterial-based biosensor by covalently coupling OPH loaded on quantum dots with carbon nanotubes or gold composites.…”

Section: Organophosphoric Pesticide Sensingmentioning

confidence: 99%

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Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

et al. 2021

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Among the many biological entities employed in the development of biosensors, enzymes have attracted the most attention. Nanotechnology has been fostering excellent prospects in the development of enzymatic biosensors, since enzyme immobilization onto conductive nanostructures can improve characteristics that are crucial in biosensor transduction, such as surface-to-volume ratio, signal response, selectivity, sensitivity, conductivity, and biocatalytic activity, among others. These and other advantages of nanomaterial-based enzymatic biosensors are discussed in this work via the compilation of several reports on their applications in different industrial segments. To provide detailed insights into the state of the art of this technology, all the relevant concepts around the topic are discussed, including the properties of enzymes, the mechanisms involved in their immobilization, and the application of different enzyme-derived biosensors and nanomaterials. Finally, there is a discussion around the pressing challenges in this technology, which will be useful for guiding the development of future research in the area.

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Section: Environmental Applicationsmentioning

confidence: 99%

Section: Environmental Applicationsmentioning

confidence: 99%

Section: Heavy Metal Sensingmentioning

confidence: 99%

Section: Organophosphoric Pesticide Sensingmentioning

confidence: 99%

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Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

et al. 2021

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“…Molecular analysis provides meaningful information such as food quality or safety in food sciences, degree of water or air pollution in environmental sciences, and presence of disease or therapeutic effect in life sciences [1,2,3]. In particular, analyses of biomolecules have continuously been desired since biomolecules are not only essential to one or more biological processes but also their deficiency or excess can cause various diseases.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Micropatterned Interdigitated Electrodes for Enhancing the Concentration Effect Based on Dielectrophoresis

Kim

Ahn

Lee

et al. 2019

Sensors

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The concentration effect of dielectrophoresis (DEP) enables detection of biomolecules with high sensitivity. In this study, microstructures were patterned between the interdigitated microelectrodes (IMEs) to increase the concentration effect of DEP. The microstructures increased the electric field gradient (∇|E2|) between the IMEs to approximately 6.61-fold higher than in the bare IMEs with a gap of 10 μm, resulting in a decreased optimal voltage to concentrate amyloid beta 42 (Aβ42, from 0.8 Vpp to 0.5 Vpp) and tau-441 (from 0.9 Vpp to 0.6 Vpp) between the IMEs. Due to the concentration effect of DEP, the impedance change in the optimal condition was higher than the values in the reference condition at 2.64-fold in Aβ42 detection and at 1.59-fold in tau-441 detection. This concentration effect of DEP was also verified by counting the number of gold (Au) particles which conjugated with the secondary antibody. Finally, an enhanced concentration effect in the patterned IMEs was verified by measuring the impedance change depending on the concentration of Aβ42 and tau-441. Our results suggest that microstructures increase the concentration effect of DEP, leading to enhanced sensitivity of the IMEs.

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Biotechnological Advances in Detection of Contaminants from Wastewater

Mehta

Patel

Mukherjee

et al. 2022

CLEAN Soil Air Water

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Wastewater from industries causes major environmental pollution problems. The urban development and commercialization in developing countries cause deterioration of surface water resources. Hence, for its improvement, it is essential to develop highly sensitive devices with quick response for monitoring of wastewater contamination. In this respect, biotechnological processes such as biosensors can be promising and a robust option for detection of toxic pollutants. Biosensors can be applied in order to acquire real‐time quantitative data. Nowadays, the enzymes or whole cell bio‐reporters‐based biosensors have promising potential due to the possibility of miniaturization and opportunity for development of in situ biosensors. The growing need of convenient, rapid, and efficient biosensing devices explains the current advancement of biosensors with new transduction materials for multiplexed contaminant detection. Considering the extensive range of sensing strategies including immunochemical‐based, enzymatic and nonenzymatic signals, different kinds of biosensors can be classified as electrochemical, optical, thermal, etc. For the past few years, more attention has been paid to nanomaterial‐based biosensors which exhibit monitoring on the basis of chemical reactions and biological phenomena. Overall, this review focuses on principle and operation (transduction mechanism) of biosensors based on bio‐recognition elements, for the early detection of organic and inorganic contaminants existing in wastewater.

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An overview of enzyme-based biosensors for environmental monitoring

Cited by 17 publications

References 96 publications

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

Designing of Nanomaterials-Based Enzymatic Biosensors: Synthesis, Properties, and Applications

Highly Sensitive Micropatterned Interdigitated Electrodes for Enhancing the Concentration Effect Based on Dielectrophoresis

Biotechnological Advances in Detection of Contaminants from Wastewater

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