An overview of Engineering Aspects of Solid State Fermentation

Prabhakar, A.; Krishnaiah, K.; Janaun, Jidon; Bono, Awang

doi:10.21161/mjm.120502

Cited by 15 publications

(34 citation statements)

References 15 publications

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“…Solid‐state fermentation is contemplated as a salient bioconversion technique to transform natural raw materials into a spacious diversity of biochemical by‐products. This process takes place through the agitation of solid substrates by the utilization of microorganisms in the absence of free‐falling water . Solid‐state fermentation in particular offers numerous advantages for the production of proteins because of its high productivity, low‐cost media, less effort in downstream processing and resultant products with added nutritional market value .…”

Section: Introductionmentioning

confidence: 99%

Single-cell protein production through microbial conversion of lignocellulosic residue (wheat bran) for animal feed

2015

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Agricultural residue (wheat bran) rich in carbohydrates was utilized in the fermentation process to produce microbial biomass. Single-cell biomass consists of the dried cells of microorganisms, which are used as protein supplements in human food and in animal feed. In the present study, two different microorganisms (Candida utilis and Rhizopus oligosporus) were studied for biomass production. To enhance the nutritional contents of wheat bran, a number of different fermentation parameters (effect of inoculum size, age of inoculum, incubation period, moisture to substrate ratio and incubation temperature) were optimized. Maximum yield was obtained at an inoculum size of 10% (v/w), with the age of the inoculum being a 48 h old culture. A fermentation period of 48 h was found to give the maximum protein yield and viable counts of yeast cells and mould hyphae. The microorganisms showed good growth at 30°C. After complete optimization of the fermentation parameters, a batch of wheat bran was fermented with C. utilis and R. oligosporus under the optimized conditions, resulting in a maximum crude protein yield of 41.02% compared with the 4.21% crude protein of the non-fermented wheat bran.

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Section: Introductionmentioning

confidence: 99%

Single-cell protein production through microbial conversion of lignocellulosic residue (wheat bran) for animal feed

2015

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“…3.1. Screening of various agro based materials for L-asparaginase production in F. culmorum Solid substrate fermentation is a low cost method for the production of enzymes as it provides better process control, higher yields besides several other beneficial aspects (Prabhakar, 2005). Selection of appropriate substrates for the production of enzymes is crucial in solid substrate fermentation as it depends on several factors mainly related with the cost, nutritive value and availability of the substrate (Pandey et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

Solid state fermentation: An effective fermentation strategy for the production of L-asparaginase by Fusarium culmorum (ASP-87)

Meghavarnam

Janakiraman

2017

Biocatalysis and Agricultural Biotechnology

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Fusarium culmorum (ASP-87) isolated from tropical soil was investigated under solid state fermentation on a laboratory scale using sixty five (65) agro based materials. Among the different agro based materials evaluated, soybean meal supported maximum L-asparaginase production (7.21 U/gds). Various optimization strategies for the production of L-asparaginase were also carried out with soybean meal and it was observed that inoculum size of 1 × 10 8 spores/mL, day 6 of incubation period, 3 mm of particle size of the substrate, moisture content of 70%, initial pH of 7.0 and temperature at 30°C were found to be optimal for Lasparaginase production. Supplementation of glucose (0.1%) and ammonium chloride (0.1%) enhanced L-asparaginase production to 1.7 fold. Mixed substrate fermentation using soybean meal and wheat bran in the ratio (1:1 w/w) further enhanced production of L-asparaginase to 0.5 fold with a final yield of 18.91 U/gds.

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“…All these species are known to produce vast array of the primary and secondary metabolites [34]. In recent days, SSF appears to be one of the best choices for any metabolite production using cheaper raw materials [7,35,36]. The solid substrates used in SSF are generally insoluble in water and play a dual role by supplying of nutrients and providing anchorage to the cells [37].…”

Section: Discussionmentioning

confidence: 99%

Purification and Cytotoxicity Study of Lovastatin From Soil Fungi

Balraj

Saibaba

Archunan

et al. 2017

Asian J Pharm Clin Res

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Objective: The objective of the present study is to evaluate the anticancer potential of lovastatin obtained from fungal source.Methods: About 15 fungal cultures were isolated from soil samples collected from Bharathiar University, India, and all are identified and characterized through microscopic characterization. Lovastatin producing capability was confirmed through bioassay against Saccharomyces cerevisiae, and the ability of selected fungus to produce lovastatin was further confirmed by high-performance liquid chromatography. Maximum lovastatin producing fungi were further selected for purification (overloaded elution chromatography) and characterization done using inhibition rate (IR). 5-diphenyl tetrazolium bromide (MTT) assay using A549 cell line was performed for antitumor activity evaluation.Results: Among the 15 isolates, Aspergillus flavus exhibited the maximum zone of inhibition (1.5 cm) against the test organism through solid-state fermentation. The resemblance in retention time (RT) of peak shown in chromatograms of standard lovastatin (RT=25.1 minutes) and sample (RT=25.1 minutes) were similar. This confirmed the presence of lovastatin in the selected fungal isolate (A. flavus). The presence of two functional groups in lovastatin C=O and O-H was confirmed by IR spectrum 50% of cell lysis was observed in MTT assay.Conclusion: Lovastatin obtained from soil fungi is capable of producing lovastatin in good proportions. Obtained fungal lovastatin exhibited significant antitumor activity against A549 cell line. Like other biological derivatives, lovastatin from soil fungi had greater potential in anticancer activity, and further biosynthetic pathway insights in their production can improve the yield which aid in large scale production.

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An overview of Engineering Aspects of Solid State Fermentation

Cited by 15 publications

References 15 publications

Single-cell protein production through microbial conversion of lignocellulosic residue (wheat bran) for animal feed

Single-cell protein production through microbial conversion of lignocellulosic residue (wheat bran) for animal feed

Solid state fermentation: An effective fermentation strategy for the production of L-asparaginase by Fusarium culmorum (ASP-87)

Purification and Cytotoxicity Study of Lovastatin From Soil Fungi

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