An overview of carbapenem-resistant Klebsiella pneumoniae

Spagnolo, Anna Maria; Orlando, Paolo; Panatto, Donatella; Perdelli, Fernanda; Cristina, Maria Luisa

doi:10.1097/mrm.0b013e328365c51e

Cited by 39 publications

(43 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, most of isolates were collected from urinary tract infections. Several other previous studies confirm our results (12,13). The urine is the main route for antibiotic excretion from the body and thus, most of resistant isolates were collected from this site.…”

Section: Discussionsupporting

confidence: 93%

“…These six carbapenemase types have low similarity at the genetic level, and yet they have identical hydrolysis profiles. The bla PER occurs less frequently, but has clinical importance by conferring resistance to oxy-imino-beta-lactams (11,12). Unfortunately, several antibiotic resistance mechanisms often have been occurred simultaneously, thereby conferring multidrug-resistant phenotypes.…”

Section: Key Pointmentioning

confidence: 99%

See 1 more Smart Citation

Antibiotic resistance and presence of blaPER-1, blaVEB-1 and blaPSE-1 beta-lacamases among clinical isolates of Pseudomonas aeruginosa from ICU settings

Ghasemian¹,

Mostafavi²,

Eslami³

et al. 2018

Immunopathol Persa

View full text Add to dashboard Cite

Introduction:Pseudomonas aeruginosa isolates are among the most common pathogens causing nosocomial infections. They are intrinsically resistant to most of antibiotics such as novel β-lactams and therefore, can develop resistance during treatment, culminating in failure in remedy. Objectives: The aim of this study was to detect the genes encoding class A extended-spectrum betalactamases (ESBLs) such as PER-1, VEB-1 and PSE-1 among P. aeruginosa isolates from intensive care unit (ICU) patients. Materials and Methods: A total of 65 isolates were collected from ICU in three hospitals of Tehran in 2016. The antibiotic susceptibility test was conducted according to Clinical and Laboratory Standards Institute (CLSI) guideline. MIC of ceftazidime was done with agar dilution method. The combine disk test was performed for detection of isolates producing ESBLs. Polymerase chain reaction (PCR) was performed to detect the PER-1, VEB-1 and PSE-1 genes using specific primers. Results: Fifty-four percent (n=38) of patients were male and 46% (n=27) were female. The majority of ICU isolates were resistant to augmentin (93.8%, n=61) and cefpodoxime (84.8%, n=56). Fifty (77%) isolates were ESBL positive, among which 94% (n=47) harbored PER-1 gene followed by 52% (n=26) VEB-1 and 16% (n=8) PSE-1 genes. Conclusion: Concomitance presence of bla PER1 and bla VEB1 was observed among 10 isolates, and 7 amplified all these three genes. A high number of ICU P. aeruginosa isolates were ESBL producers. The frequency of bla VEB1 and bla PER1 were relatively high, while bla PSE1 was detected among a low number of isolates. Moreover, resistance to carbapenems was low. It is necessary to follow up ICU centers because of drugresistant P. aeruginosa isolates. AbstractCitation: Ghasemian A, Shokouhi Mostafavi SK, Eslami M, Vafaei M, Nojoomi F, Hasanvand F. Antibiotic resistance and presence of bla PER-1 , bla VEB-1 and bla PSE-1 beta-lacamases among clinical isolates of Pseudomonas aeruginosa from ICU settings. Immunopathol Persa. 2018;4(2):e26.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Key Pointmentioning

confidence: 99%

Antibiotic resistance and presence of blaPER-1, blaVEB-1 and blaPSE-1 beta-lacamases among clinical isolates of Pseudomonas aeruginosa from ICU settings

Ghasemian¹,

Mostafavi²,

Eslami³

et al. 2018

Immunopathol Persa

View full text Add to dashboard Cite

show abstract

“…2015; Pan et al, 2019). Spagnolo et al (2014) reported that K. pneumoniae is responsible for some healthcareassociated and community-associated infections. Colonization of the gastrointestinal (GI) tract is usually a pre-requisite for induction of infection, and medical personnel are culpable reservoir of infection contagion (Calbo et al, 2011).…”

Section: Research Articlementioning

confidence: 99%

Characterization and Plasmid Profile of Resistant Klebsiella pneumoniae Isolates in Patients with Urinary Tract Infection in Nasarawa State, Nigeria

Orole

Hadi

2020

Int J Appl Sci Biotechnol

View full text Add to dashboard Cite

Klebsiella pneumoniae has been identified as an urgent threat to human health based on its increasing antimicrobial resistance to the beta-lactamases and Carbapenemases. The pathogen has become a threat to both patients and healthcare providers as its incidence is on the increase, becoming a major global healthcare issue. The study was aimed at characterizing and determining the plasmid profile of resistant Klebsiella pneumoniae isolates from urinary tract of patients at Dalhatu Araf Specialist Hospital Lafia, Nasarawa State, Nigeria. Early morning mid-stream urine samples were collected from patients with urinary tract infections between April and May, 2019 and Klebsiella pneumoniae characterized on the basis of its antibiotic resistance pattern, and the plasmid DNA profile determined. Thirty-eight strains of resistant Klebsiella pneumoniae were obtained of which 33 showed resistance to more than three antibiotics. About 51.1% of the isolates were resistant to Tetracycline, while the isolates were least resistant to Azithromycin and Cefotaxime (30.3%) respectively. Klebsiella pneumoniae isolates showed 32 different resistance patterns, 24 of the strains had the capacity to produce Extended Spectrum Beta-lactamases enzymes: CTX-M 24(72.7%), SHV 19(57.6%) and TEM 16(48.5%) respectively. All the resistant Klebsiella pneumoniae isolated had the same plasmid size of 48.5 kilobases and only 1 plasmid each though they all obtained a multiple antibiotic resistance (MAR) index > 0.2. The study concluded that Klebsiella pneumoniae harbours genes which confer antibiotic resistance on the isolates. The study exposes further the challenge of antibiotic resistance and need for concerted effort at stopping the challenge of drug resistance. Int. J. Appl. Sci. Biotechnol. Vol 8(1): 21-28

show abstract

“…CPKp causes Carbapenem resistance to K. pneumoniae strains by plasmid-encoded carbapenemase enzymes. CPKp can produce metallo-β-lactamases which include New Delhi metallo-β-lactamase, Imipenemase metallo-β-lactamase and Verona integron-encoded metallo-β-lactamase, in addition to Oxacillin-type Carbapenemases (class D) [10].…”

Section: Introductionmentioning

confidence: 99%

Colistin Susceptibility in Carbapenem Resistant Klebsiella Pneumoniae and their Ability of Biofilm Formation

Kadum

2020

eijs

View full text Add to dashboard Cite

A total of 157 clinical samples were collected from different clinical specimens (urine, sputum, blood, swabs, and cannula) from several hospitals in Iraq. Among the samples, 51 isolates (32.48%) of Klebsiella pneumoniae were identified according to morphologicaland cultural characteristics as well as the Enterosystem 18R test. Higher numbers of K. pneumoniae isolates were observed in urine samples (26, 52%) than the other samples, and in females (70.6%) than males (29.4%) (female: male ratio of about 2.4:1). Antibiotic susceptibility of K. pneumoniae against 12 commonly used antibiotics was determined through the disc-diffusion method. The results revealed a higher resistance rate in 51 isolates (100%) against Cephalexin, followed by Ceftazidime (50, 98%), while the lowest resistance rate (24, 47%) was against each of Imipenem and Meropenem. Also, the investigation of the minimum inhibitory concentration (MIC) of Colistin using E-test (strips) demonstrated that 33 isolates were resistance, as compared to 31 using the disk diffusion assay. DNA was extracted from K. pneumoniae isolates and molecularly tested using polymerase chain technique (PCR) with a specific primer and 108 bp product to detect the rpoB gene that represents this bacteria . Also, all of the 51 isolates of K. pneumoniae identified by the rpoB gene were detected for the expression of the Colistin drug resistance gene mgr-B , which was amplified (347 bp) using a specific primer. Colistin resistance gene mgr-B was amplified and sequenced from the twenty isolates. Only 6 isolates appeared with a single nucleotide substitution; G instead A, A instead G, C instead G and G instead C. Also, this study tested biofilm formation from K. pneumoniae isolates , using the microtiter plate method, in association with Colistin and Carbapenem resistant. The Colistin and Carbapenem resistance pattern was compared to the ability of biofilmformation as weak formation versus strong and also, Multi-drug resistant isolates were more common among weak versus strong biofilm formers.

show abstract

An overview of carbapenem-resistant Klebsiella pneumoniae

Cited by 39 publications

References 48 publications

Antibiotic resistance and presence of blaPER-1, blaVEB-1 and blaPSE-1 beta-lacamases among clinical isolates of Pseudomonas aeruginosa from ICU settings

Antibiotic resistance and presence of blaPER-1, blaVEB-1 and blaPSE-1 beta-lacamases among clinical isolates of Pseudomonas aeruginosa from ICU settings

Characterization and Plasmid Profile of Resistant Klebsiella pneumoniae Isolates in Patients with Urinary Tract Infection in Nasarawa State, Nigeria

Colistin Susceptibility in Carbapenem Resistant Klebsiella Pneumoniae and their Ability of Biofilm Formation

Contact Info

Product

Resources

About