An overview about varieties and detection methods of pathogenic microorganisms in indoor air

Li, Xiaoxu; Weng, Zufeng; Aili, Cao; Liu, Qi; Sui, Guodong

doi:10.1360/n972018-00328

Cited by 9 publications

(7 citation statements)

References 48 publications

(66 reference statements)

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“…Basidiomycetes depend on culture methods [43], and this characteristic explains the low number of Basidiomycota isolates in this work. The culture-dependent method might have overlooked some unculturable endophytic fungi [44], but many isolates could still be separated [39]. We obtained abundant endophytic fungal isolates from S. suffruticosa, which was conducive for screening the active strains and laying a foundation for their application.…”

Section: Discussionmentioning

confidence: 99%

Diversity and antimicrobial activity of endophytic fungi isolated from Securinega suffruticosa in the Yellow River Delta

Yao

et al. 2020

PLoS ONE

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Securinega suffruticosa (Pall.) Rehd is an excellent natural secondary shrub in the Shell Islands of Yellow River Delta. The roots of S. suffruticosa have high medicinal value and are used to treat diseases, such as neurasthenia and infant malnutrition. Any organism that is isolated from this species is of immense interest due to its potential novel bioactive compounds. In this research, the distribution and diversity of culturable endophytic fungi in S. suffruticosa were studied, and the endophytic fungi with antimicrobial activity were screened. A total of 420 endophytic fungi isolates were obtained from the S. suffruticosa grown in Shell Islands, from which 20 genera and 35 species were identified through morphological and internal transcribed spacer (ITS) sequence analyses. Chaetomium, Fusarium, Cladosporium, and Ceratobasidium were the dominant genera. The high species richness S (42), Margalef index D 0 (5.6289), Shannon-Wiener index H 0 (3.1000), Simpson diversity index D s (0.9459), PIE index (0.8670), and evenness Pielou index J (0.8719) and a low dominant index λ (0.0541) indicated the high diversity of endophytic fungi in S. suffruticosa, the various species of endophytic fungi with obvious tissue specificity. The inhibition percentages of the 12 species of such endophytic fungi against Colletotrichum siamense were 3.6%-26.3%. C. globosum, Fusarium sp.3, and C. ramotenellum had a high antibacterial activity against Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) were between 0.5 mg/mL and 2 mg/mL. Alkaloid content detection indicated that endophytic fungi had a high alkaloid content, whereas the alkaloid contents of C. globosum and Fusarium sp.3 reached 0.231% and 0.170%, respectively. Members belonging to the endophytic fungal community in the S. suffruticosa of Shell Islands that may be used as antagonists and antibacterial agents for future biotechnology applications were identified for the first time.

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Section: Discussionmentioning

confidence: 99%

Diversity and antimicrobial activity of endophytic fungi isolated from Securinega suffruticosa in the Yellow River Delta

Yao

et al. 2020

PLoS ONE

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“…All of these are not conducive to the early warning and prevention of pathogenic microorganisms. There are still many challenges in the rapid detection and accurate qualitative and quantitative analysis of airborne microorganisms [ 86 ]. The development of microfluidic chips meets the needs of scientific research and the market, combined with nucleic acid extraction, protein extraction, PCR amplification, DNA sequencing and immunoassay detection technologies, and they can be used for the detection of airborne microorganisms [ 87 , 88 ].…”

Section: Detection Of Airborne Microorganisms By Microfluidic Chipsmentioning

confidence: 99%

Application of Microfluidic Chips in the Detection of Airborne Microorganisms

et al. 2022

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The spread of microorganisms in the air, especially pathogenic microorganisms, seriously affects people’s normal life. Therefore, the analysis and detection of airborne microorganisms is of great importance in environmental detection, disease prevention and biosafety. As an emerging technology with the advantages of integration, miniaturization and high efficiency, microfluidic chips are widely used in the detection of microorganisms in the environment, bringing development vitality to the detection of airborne microorganisms, and they have become a research highlight in the prevention and control of infectious diseases. Microfluidic chips can be used for the detection and analysis of bacteria, viruses and fungi in the air, mainly for the detection of Escherichia coli, Staphylococcus aureus, H1N1 virus, SARS-CoV-2 virus, Aspergillus niger, etc. The high sensitivity has great potential in practical detection. Here, we summarize the advances in the collection and detection of airborne microorganisms by microfluidic chips. The challenges and trends for the detection of airborne microorganisms by microfluidic chips was also discussed. These will support the role of microfluidic chips in the prevention and control of air pollution and major outbreaks.

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“…H1N1 virus caused influenza pandemic in 2009 [29]. Varicella-zoster [45,46] 80-120 nm Up to 21 days Up to 11 days E. coli [47] 1 -2 μm Up to 1 h Up to 16 months Staphylococci [46] 500 nm-1 μm Up to 3 days Up to 7 months Streptococci [45] 500 nm-2 μm Up to 2 days Up to 6.5 months Aspergillus and penicillium conidia [47] 2-4μm Up to 22 years Up to 22 years virus, measles virus, mumps virus and rubella virus can also spread in aerosol form [2].…”

Section: Hazards Of Pbasmentioning

confidence: 99%

“…On the one hand, about 90 percent of nosocomial infections are caused by bacteria. The hospital environment and medical equipment are the main sources of these microbes [2]. Recently, as the application of broad-spectrum antibiotics and minimally invasive treatment techniques, the frequency of nosocomial infections caused by antibiotic-resistant Grampositive bacteria, such as methicillin resistant Staph., Enterococci, and Coagulase-negative staphylococci, keep increasing [31].…”

Section: Hazards Of Pbasmentioning

confidence: 99%

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Atmospheric-pressure non-equilibrium plasmas for effective abatement of pathogenic biological aerosols

Wang

Chen

et al. 2021

Plasma Sources Sci. Technol.

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The COVID-19, viral influenza, tuberculosis, and other widespread infectious diseases evidence that pathogenic biological aerosols (PBAs) are a serious threat to public health. Different from traditional inactivation methods, such as ultraviolet (UV) light which are only safe to use when people are not present, and high-efficiency particulate filters (HEPA) which merely filter microbes without killing them, atmospheric pressure nonequilibrium plasma (APNP) has shown its tremendous potential in drastically diminishing the aerosol transmission route of the infectious agents through the abatement of PBAs. The key issues to develop high performance APNP based air purification system are critically reviewed. Systematic studies on the hazards of different PBAs and the spread of PBAs in indoor environments guide the development of APNP sources to control communicable diseases. The key six sampling and seven detection methods on PBAs are introduced to analyze the PBA abatement efficiency by APNP. Seven common APNP sources which can remove viruses and bacteria aerosols efficiently developed during the past 8 years are introduced. For the APNP sources with small plasma volume, the electric field and diffusion driven charging are the dominant mechanisms to charge PBAs, while the common methods of dusty plasma research can be adapted to atmospheric-pressure conditions to describe the charging effects of APNP sources with large plasma volume. Plentiful long- and short-lifetime reactive oxygen and nitrogen species (RONS) generated by APNP effectively contribute to inactivation of bacterial aerosols. Current studies suggest that viral aerosols are mainly inactivated by short-lifetime RONS including 1O2, ONOO− and ONOOH. The study on the dissolution and reaction of gaseous RONS in microdroplets and accurate measurements on the evolution of charged PBAs are envisaged to be the focus of future research. Opportunities for multidisciplinary collaborative research to advance the development of next-generation high-performance plasma-based air purifiers are highlighted.

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An overview about varieties and detection methods of pathogenic microorganisms in indoor air

Cited by 9 publications

References 48 publications

Diversity and antimicrobial activity of endophytic fungi isolated from Securinega suffruticosa in the Yellow River Delta

Diversity and antimicrobial activity of endophytic fungi isolated from Securinega suffruticosa in the Yellow River Delta

Application of Microfluidic Chips in the Detection of Airborne Microorganisms

Atmospheric-pressure non-equilibrium plasmas for effective abatement of pathogenic biological aerosols

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