An outbreak of<i>Escherichia coli</i>O157 associated with a children's paddling pool

Brewster, David H.; Brown, Margarita; Robertson, David; Houghton, G. L.; Bimson, J.; Sharp, J. C. M.

doi:10.1017/s0950268800051141

Cited by 71 publications

(43 citation statements)

References 15 publications

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“…H7 has been isolated from cattle and foods of animal origin (contaminated meat, unpasteurized milk) [2]. Waterborne and person-to-person transmission are also believed to be modes of spread of this pathogen [7][8][9]. Several phenotypic and genotypic schemes have been developed to assist in epidemiological investigations.…”

Section: Introductionmentioning

confidence: 99%

Characterization of verocytotoxin-producingEscherichia coliO157 isolates from patients with haemolytic uraemic syndrome in Western Europe

Heuvelink¹,

Kar

Meis³

et al. 1995

Epidemiol. Infect.

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Section: Introductionmentioning

confidence: 99%

Characterization of verocytotoxin-producingEscherichia coliO157 isolates from patients with haemolytic uraemic syndrome in Western Europe

Heuvelink¹,

Kar

Meis³

et al. 1995

Epidemiol. Infect.

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“…within the Enterobacteriaceae are human waterborne pathogens found in domestic wastewater. In particular, Escherichia coli strains constitute an important set of waterborne pathogens composed of numerous Shiga toxin-producing strains and enterohemorrhagic (e.g., O157: H7) strains associated with potable (14,32) and recreational (7,22) water.…”

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confidence: 99%

Waterborne Pathogen Detection by Use of Oligonucleotide-Based Microarrays

Maynard

Berthiaume

Lemarchand

et al. 2005

Appl Environ Microbiol

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A small-oligonucleotide microarray prototype was designed with probes specific for the universal 16S rRNA and cpn60 genes of several pathogens that are usually encountered in wastewaters. In addition to these two targets, wecE-specific oligonucleotide probes were included in the microarray to enhance its discriminating power within the Enterobacteriaceae family. Universal PCR primers were used to amplify variable regions of 16S rRNA, cpn60, and wecE genes directly in Escherichia coli and Salmonella enterica serovar Typhimurium genomic DNA mixtures (binary); E. coli, S. enterica serovar Typhimurium, and Yersinia enterocolitica genomic DNA mixtures (ternary); or wastewater total DNA. Amplified products were fluorescently labeled and hybridized on the prototype chip. The detection sensitivity for S. enterica serovar Typhimurium was estimated to be on the order of 0.1% (10 4 S. enterica genomes) of the total DNA for the combination of PCR followed by microarray hybridization. The sensitivity of the prototype could be increased by hybridizing amplicons generated by PCR targeting genes specific for a bacterial subgroup, such as wecE genes, instead of universal taxonomic amplicons. However, there was evidence of PCR bias affecting the detection limits of a given pathogen as increasing amounts of a different pathogen were spiked into the test samples. These results demonstrate the feasibility of using DNA microarrays in the detection of waterborne pathogens within mixed populations but also raise the problem of PCR bias in such experiments.

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“…Recreational water related cases have sporadically been reported in the North America and in the United Kingdom and pool-borne ones are always linked to small paddling pools and other, small nonchlorinated facilities [19,20]. A review of 20 years E. coli O157:H7 epidemiology in the USA revealed a total of 350 outbreaks with 31 (9%) recreational water related ones, a third of which linked to swimming pools [21].…”

Section: Bacteriamentioning

confidence: 99%

The risk of contracting infectious diseases in public swimming pools. A review

Barna

Kádár

2012

Annali Dell'Istituto Superiore Di Sanità

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Summary.A review of pathogenic microorganisms presenting risk of infection in pool based artificial recreational water venues is extracted from the available scientific literature. The microorganisms are grouped both according to their way of spread and their survival and growth strategies and their characteristics relevant for the pool and spa based recreation are discussed. In order to put the proposed risks on a solid basis, among others a ten year excerpt of the waterborne disease statistics of the Centers for Disease Control and Prevention (CDC) is used throughout the article.Key words: man-made water recreational environment, risk of infection, waterborne disease outbreaks, swimming pool, hot tub.Riassunto (Il rischio di contrarre malattie infettive nelle piscine pubbliche. Una rassegna). Viene presentata una sintesi della letteratura scientifica disponibile sui microorganismi patogeni potenzialmente infettivi, in ambienti acquatici ricreativi artificiali, come le piscine. I microorganismi sono raggruppati sia in base alle loro vie di diffusione che in base alle loro strategie di sopravvivenza e di crescita; successivamente vengono discusse le loro caratteristiche più importanti negli ambienti ricreativi considerati, piscine e terme. Per fornire adeguate e solide basi scientifiche ai tipi di rischi presentati, vengono analizzate, oltre ad altri documenti, le statistiche relative a dieci anni di osservazione di malattie legate all'acqua del Centers for Disease Control and Prevention (CDC).Parole chiave: ambienti ricreativi acquatici artificiali, rischio di infezione, epidemie di malattie legate all'acqua, piscine, vasche da idromassaggio.

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An outbreak ofEscherichia coliO157 associated with a children's paddling pool

Cited by 71 publications

References 15 publications

Characterization of verocytotoxin-producingEscherichia coliO157 isolates from patients with haemolytic uraemic syndrome in Western Europe

Characterization of verocytotoxin-producingEscherichia coliO157 isolates from patients with haemolytic uraemic syndrome in Western Europe

Waterborne Pathogen Detection by Use of Oligonucleotide-Based Microarrays

The risk of contracting infectious diseases in public swimming pools. A review

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