An Organotypic Assay to Study Epithelial-Fibroblast Interactions in Human Breast

Sumbal, Jakub; Gudjonsson, Thorarinn; Traustadóttir, Gunnhildur Ásta; Koledová, Zuzana

doi:10.1007/978-1-0716-2193-6_16

Cited by 2 publications

(1 citation statement)

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“…Thus, MEC-derived organoids are a suitable system to assess the effect of controlled developmental signals, but should be used with the previously discussed considerations. Future studies involving the addition of signals that contribute to endogenous mammary gland development and maintenance, along with co-culturing with essential cells from the mammary microenvironment will further improve the fidelity of this system (Koledova & Lu, 2017;Sumbal et al, 2021Sumbal et al, , 2022. Moreover, assessment of secreted factors by these non-epithelial cells and estimation of direct cell-cell communication dynamics would provide valuable insights.…”

Section: Discussionmentioning

confidence: 99%

Single-cell transcription mapping of murine and human mammary organoids responses to female hormones

Ortiz,

Lewis,

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et al. 2023

Preprint

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During female adolescence and pregnancy, rising levels of hormones result in a cyclic source of signals that control the development of mammary tissue. While such alterations are well understood from a whole-gland perspective, the alterations that such hormones bring to organoid cultures derived from mammary glands have yet to be fully mapped. This is of special importance given that organoids are considered suitable systems to understand cross species breast development. Here we utilized single-cell transcriptional profiling to delineate responses of murine and human normal breast organoid systems to female hormones across evolutionary distinct species. Collectively, our study represents a molecular atlas of epithelial dynamics in response to estrogen and pregnancy hormones.

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Section: Discussionmentioning

confidence: 99%