An optimized procedure to develop a 3‐dimensional microfluidic hydrogel with parallel transport networks

Jafarkhani, Mahboubeh; Salehi, Zeinab; Shokrgozar, Mohammad Ali; Mashayekhan, Shohreh

doi:10.1002/cnm.3154

Cited by 3 publications

(5 citation statements)

References 29 publications

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“…Micro-channels were embedded in collagen hydrogel according to the needle-based microfluidics fabrication method [33]. Briefly, 2% (w/v) collagen was dissolved in 1% HCl solution.…”

Section: Fabrication Of the Microfluidic Platformmentioning

confidence: 99%

“…Adult bovine heart muscle was decellularized as previously described [33]. In brief, the sample was washed for 2 h with PBS and then, placed in SDS solution (0.1% w/v) for 5 days.…”

Section: Decellularization Of the Bovine Heart Tissuementioning

confidence: 99%

“…The average pore size was determined by analyzing SEM images based on measuring at least 500 randomly chosen pores using Image J software. The porosity of the scaffolds was calculated by the liquid displacement method [33].…”

Section: Scanning Electron Microscope (Sem) Analysismentioning

confidence: 99%

“…Continuous perfusion of culture media, at an average flow rate of 6 µl min −1 , was created by the difference between hydrostatic pressures in the inlet and outlet reservoirs. It is necessary to explain that due to the limitation of the system dimensions [33] and the need for optimal use of the culture medium (economic considerations), the hydrostatic pressure difference between the inlet and outlet was considered within the range of 1.5 to 2 cm, creating an average flow of about 6 µl min −1 in all channels.…”

Section: Fabrication Of the Microfluidic Platformmentioning

confidence: 99%

“…Different amounts of CS were dissolved in dilute acetic acid (1% v/v) and mixed at 70 • C for 24 h. DT was extracted by enzymatic digestion as described elsewhere [33]. DT was dispersed in an HCl solution (0.01 M) containing 1 mg ml −1 pepsin in agitation conditions for 60 h at room temperature.…”

Section: Fabrication Of the Hydrogel Sheetsmentioning

confidence: 99%

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Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Jafarkhani¹,

Salehi²,

Mashayekhan³

et al. 2020

Biomed. Mater.

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Endothelial cell migration is a crucial step in the procedure of new blood vessel formation-a necessary process to maintain cell viability inside thick tissue constructs. Here, we report a new method for maintaining cell viability and inducing cell migration using a perfused microfluidic platform based on collagen gel and a gradient hydrogel sheet. Due to the helpful role of the extracellular matrix (ECM) components in cell viability, we developed a hydrogel sheet from decellularized tissue (DT) of the bovine heart and chitosan (CS). The results showed that the hydrogel sheets with an optimum weight ratio of CS/DT=2 possess a porosity of around 75%, a mechanical strength of 23 kPa, and display cell viability up to 78%. Then, we immobilized a radial gradient of vascular endothelial growth factor (VEGF) on the hydrogel sheet to promote human umbilical vein endothelial cells (HUVECs) migration. Finally, we incorporated the whole system as an entirety on the top of the microfluidic platform and studied cell migration through the hydrogel sheet in the presence of soluble and immobilized VEGF. The results demonstrated that immobilized VEGF stimulated cell migration in the hydrogel sheet at all depths compared with soluble VEGF. The results also showed that applying a VEGF gradient in both soluble and immobilized states had a significant effect on cell migration at limited depths (<100 μm). The main finding of this study is a significant improvement in cell migration using an in vivo imitating, costefficient, and highly reproducible platform, which may open up a new perspective for tissue engineering applications.

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“…Micro-channels were embedded in collagen hydrogel according to the needle-based microfluidics fabrication method [33]. Briefly, 2% (w/v) collagen was dissolved in 1% HCl solution.…”

Section: Fabrication Of the Microfluidic Platformmentioning

confidence: 99%

“…Adult bovine heart muscle was decellularized as previously described [33]. In brief, the sample was washed for 2 h with PBS and then, placed in SDS solution (0.1% w/v) for 5 days.…”

Section: Decellularization Of the Bovine Heart Tissuementioning

confidence: 99%

Section: Scanning Electron Microscope (Sem) Analysismentioning

confidence: 99%

Section: Fabrication Of the Microfluidic Platformmentioning

confidence: 99%

Section: Fabrication Of the Hydrogel Sheetsmentioning

confidence: 99%

See 3 more Smart Citations

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Jafarkhani¹,

Salehi²,

Mashayekhan³

et al. 2020

Biomed. Mater.

Self Cite

View full text Add to dashboard Cite

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Overview of research on additive manufacturing of hydrogel-assisted lab-on-chip platforms for cell engineering applications in photodynamic therapy research

Cieślak,

Krakos,

Kulbacka

et al. 2024

Microchim Acta

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Lab-on-chips supported by hydrogel matrices are excellent solutions for cell culture; thus, this literature review presents examples of scientific research in this area. Several works are presenting the properties of biocompatible hydrogels that mimic the cellular environment published recently. Hydrogels can also be treated as cell transporters or as a structural component of microfluidic devices. The rapidly growing scientific sector of hydrogel additive manufacturing is also described herein, with attention paid to the appropriate mechanical and biological properties of the inks used to extrude the material, specifically for biomedical purposes. The paper focuses on protocols employed for additive manufacturing, e.g., 3D printing parameters, calibration, ink preparation, crosslinking processes, etc. The authors also mention potential problems concerning manufacturing processes and offer example solutions. As the novel trend for hydrogels enriched with several biocompatible additives has recently risen, the article presents examples of the use of high-quality carbon nanotubes in hydrogel research enhancing biocompatibility, mechanical stability, and cell viability. Moving forward, the article points out the high applicability of the hydrogel-assisted microfluidic platforms used for cancer research, especially for photodynamic therapy (PDT). This innovative treatment strategy can be investigated directly on the chip, which was first proposed by Jędrych E. et al. in 2011. Summarizing, this literature review highlights recent developments in the additive manufacturing of microfluidic devices supported by hydrogels, toward reliable cell culture experiments with a view to PDT research. This paper gathers the current knowledge in these intriguing and fast-growing research paths. Graphical abstract

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Microphysiological system modeling pericyte-induced temozolomide resistance in glioblastoma

Maity,

Jewell,

Yilgor

et al. 2024

Preprint

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Glioblastoma (GBM) is a malignancy with poor survival and high rates of chemoresistance. Temozolomide (TMZ), the standard-of-care chemotherapy for GBM patients, but GBM cells can be resistant to TMZ, resulting in limited clinical efficacy. Elucidating the complex mechanisms of TMZ chemoresistance in GBM requires novel in vitro models replicating the complex tumor microenvironment (TME). We present an multicellular 3D GBM model recapitulating the biomechanical characteristics of brain tissues and pericyte-mediated TMZ resistance. The composite hydrogel used to encapsulate GBM spheroids (U87, LN229, and PDM140), pericytes, or GBM spheroids with pericytes, mimics the rheological properties of brain tissues. When untreated, the GBM models remain viable and proliferative for 14 days. PDM140 spheroids were most sensitive to TMZ (IC50=73μM), followed by LN229 (IC50=278μM) and U87 (IC50=446μM). With pericytes, the viability of TMZ-treated GBM spheroids significantly increases by 22.7% for PDM140, 32.5% for LN229, and 22.1% for U87, confirming pericyte-induced GBM chemoresistance responses. The upregulation (380-fold) of C-C motif chemokine ligand 5 (CCL5) in pericytes upon TMZ treatment could explain the chemoresistance responses. This innovative brain-mimicking 3D GBM model represents a novel in vitro platform for testing the efficacy of TMZ and novel drugs targeting CCL5-mediated chemoresistance pathways in GBM.

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An optimized procedure to develop a 3‐dimensional microfluidic hydrogel with parallel transport networks

Cited by 3 publications

References 29 publications

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Retracted: Induced cell migration based on a bioactive hydrogel sheet combined with a perfused microfluidic system

Overview of research on additive manufacturing of hydrogel-assisted lab-on-chip platforms for cell engineering applications in photodynamic therapy research

Microphysiological system modeling pericyte-induced temozolomide resistance in glioblastoma

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