An optimized non-destructive protocol for testing mechanical properties in decellularized rabbit trachea

Hondt, Margot Den; Vanaudenaerde, Bart M.; Maughan, Elizabeth F.; Butler, Colin R.; Crowley, Claire; Verbeken, Eric; Verleden, Stijn E.; Vranckx, Jan

doi:10.1016/j.actbio.2017.07.035

Cited by 24 publications

(25 citation statements)

References 37 publications

(58 reference statements)

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“…Institution of a protocol to accomplish ingrowth of multiple cell types may be the key to creating implant‐ready grafts. The updates in creating tracheal grafts have been demonstrated through protocol improvements in physical decellularization, chemical decellularization, and bioreactor methods in the past five years 73–77 . These protocol advancements have also been seen in other organ systems using animal‐based models such as esophagus (murine‐rat), 78 lung (ovine), 79 cartilage (porcine), 80 uterus (ovine and leporine), 81,82 bladder (leporine), 83 pericardium (bovine), 84 submillimeter vasculature (murine‐rat), 85 and intestine (murine‐mouse) 86 model tissues.…”

Section: Extracellular Structural Matrix Functionsmentioning

confidence: 99%

Decellularized scaffolds for tissue engineering: Current status and future perspective

Rajab¹,

O’Malley

Tchantchaleishvili

2020

Artificial Organs

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Based on Organ Procurement and Transplantation Network data as of December 2019, more than 113 000 patients require an organ transplantation, yet, over the course of this past year, only 36 000 patients received a transplant. This discrepancy between those that need a donor organ and those that receive one remains one of medicine’s biggest challenges. Multiple solutions, both biologic and artificial, have been proposed to mitigate this difference. One of the most promising approaches to generate bioartificial organs for transplantation involves re‐seeding decellularized scaffolds with appropriate cells. Decellularization involves physical, chemical, or biological methods that typically require intimate contact of various decellularization solutions with each cell. Consequently, conventional submersion decellularization has been limited to simple tissues such as heart valves. The invention of perfusion decellularization was a breakthrough that allowed the generation of tissue‐engineered scaffolds from tissues with higher structural organization and entire organs. Such scaffolds are composed of a myriad of extracellular matrix (ECM) components that include collagen, elastin, proteoglycans, and glycoproteins. Together, these components allow the scaffolds to fulfill specialized functions, such as structural functions as well as biological functions including the regulation of cellular processes and extracellular molecules. These specialized functions of decellularized scaffolds can increasingly be harnessed for applications in tissue engineering.

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Section: Extracellular Structural Matrix Functionsmentioning

confidence: 99%

Decellularized scaffolds for tissue engineering: Current status and future perspective

Rajab¹,

O’Malley

Tchantchaleishvili

2020

Artificial Organs

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“…Consequently, a more comprehensive and exhaustive evaluation of acellular scaffolds is needed to ensure reproducibility, efficacy of cell removal, and maintenance of ECM integrity. To address these shortcomings, new techniques have been developed to test the mechanical properties of the scaffold (Den Hondt et al, ) and to measure the amount of decellularization reagents remaining after decellularization (Dettin et al, ). Knowledge of the critical micelle concentration of detergents (Stetsenko & Guskov, ) should aid in the development of standardized decellularization solutions that prevent micelle formation and thereby the risk of deleterious protein extraction.…”

Section: Translational Limitations Of Biological Scaffoldsmentioning

confidence: 99%

Limitations of recellularized biological scaffolds for human transplantation

Bilodeau

Goltsis

Rogers

et al. 2020

J Tissue Eng Regen Med

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A shortage of donor organs for transplantation and the dependence of the recipients on immunosuppressive therapy have motivated researchers to consider alternative regenerative approaches. The answer may reside in acellular scaffolds generated from cadaveric human and animal tissues. Acellular scaffolds are expected to preserve the architectural and mechanical properties of the original organ, permitting cell attachment, growth, and differentiation. Although theoretically, the use of acellular scaffolds for transplantation should pose no threat to the recipient's immune system, experimental data have revealed significant immune responses to allogeneic and xenogeneic transplanted scaffolds. Herein, we review the various factors of the scaffold that could trigger an inflammatory and/or immune response, thereby compromising its use for human transplant therapy. In addition, we provide an overview of the major cell types that have been considered for recellularization of the scaffold and their potential contribution to triggering an immune response.

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“…Deselülerizasyon için en az immün yanıtı oluşturacak ve mekanik özelliği doğal trakea ile yakından uyumlu olacak döngü tercih edilmiştir. 27 Bir diğer trakea deselülerizasyon çalışması 2015 yılında, Kutten ve ark. tarafından fare trakeaları kullanılarak ortaya konmuştur.…”

Section: Solunum Si̇stemi̇ Doku Mühendi̇sli̇ği̇unclassified