An optimized method for automated analysis of algal pigments by HPLC

Leeuwe, van Maria; Villerius, L.; Roggeveld, Jan; Visser, Ronald J. W.; Stefels, Jacqueline

doi:10.1016/j.marchem.2006.05.003

Cited by 89 publications

(62 citation statements)

References 22 publications

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“…The extract dissolved in methanol showed instability, mainly in first 12 h with 47% reduction in concentration content. This result for methanol confirms instability obtained by van Leeuwe et al 30 for five microalgae species.…”

Section: Pigments Extract Stabilitysupporting

confidence: 92%

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Improvement of the Extraction Process for High Commercial Value Pigments fromDesmodesmussp. Microalgae

Soares¹,

Júnior²,

Lopes³

et al. 2016

Journal of the Brazilian Chemical Society

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Considering the high interest and commercial value of pigments from microalgae, this study aimed to stablish the best lutein and β-carotene extraction method for Desmodesmus sp., a microalgae with high biomass production. The evaluation was done considering the best solvent or solvent mixture, number of extractions, pre-treatment of dry biomass with acetone, saponification in methanol and ethanol and stability evaluation of the obtained extract. Among achieved results, the best solvent extractor was a hexane:ethanol mixture in a 1:1 (v/v) proportion. In order to obtain maximum yield, the pattern stablished includes six extractions performed for 40 minutes. Stability tests presented that xanthophylls and carotenes pigments were more stable in methanol and that xanthophylls and carotenes pigments were less negatively affected than chlorophylls in the methanolic extract. Chromatographic identification and quantification of pigments from Desmodesmus sp. microalgae revealed that its major constituents are trans-zeaxanthin, chlorophyll b, β-carotene and trans-lutein.

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Section: Pigments Extract Stabilitysupporting

confidence: 92%

“…Pigments were quantified by HPLC using calibration curve from chlorophyll a, trans-lutein and trans-β-carotene standards, with six different concentrations (1,5,10,15,30 and 60 µg mL −1 ). The curve was prepared plotting standard concentration vs. area.…”

Section: Pigments Quantificationmentioning

confidence: 99%

Improvement of the Extraction Process for High Commercial Value Pigments fromDesmodesmussp. Microalgae

Soares¹,

Júnior²,

Lopes³

et al. 2016

Journal of the Brazilian Chemical Society

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“…Extraction was conducted by adding 5 mL of 90% cold acetone into the sample at 4°C for 48 h. After extraction, the sample was analyzed using HPLC using a Waters (Model 2695) system, a cooled auto-sampler (4°C), and a Waters 996 diode-array detector. The freezedrying, extraction, and HPLC analysis methods are based on van Leeuwe et al (2006). The volumetric fucoxanthin or chlorophyll-a productivity was calculated based on Eq.…”

Section: Pigment Analysismentioning

confidence: 99%

Environmental and nutrient conditions influence fucoxanthin productivity of the marine diatom Phaeodactylum tricornutum grown on palm oil mill effluent

et al. 2018

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Palm oil mill effluent (POME) is a type of wastewater posing large problems when discharged in the environment. Yet, due to its high nutrient content, POME may offer opportunities for algal growth and subsequent harvesting of high-value products. The marine diatom Phaeodactylum tricornutum is a potential feedstock diatom for bioactive compounds such as the carotenoid fucoxanthin, which has been shown to have pharmaceutical applications. The aim of this paper was to evaluate the growth and fucoxanthin productivity of P. tricornutum grown on POME, as a function of light intensity, temperature, salinity, and nutrient enrichment. High-saturating irradiance (300 μmol photons m −2 s −1) levels at 25°C showed highest growth rates but decreased the fucoxanthin productivity of P. tricornutum. Box-Behnken response surface methodology revealed that the optimum fucoxanthin productivity was influenced by temperature, salinity, and the addition of urea. Nutrient enrichment by phosphorus did not enhance cell density and fucoxanthin productivity, while urea addition was found to stimulate both. We conclude that POME wastewater, supplemented with urea, can be considered as the potential medium for P. tricornutum to replace commercial nutrients while producing high amounts of fucoxanthin.

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“…Filters were freezedried, extracted in 90% acetone and measured with a Waters liquid chromatography HPLC system (Model 2690) following the protocol of van Leeuwe et al (2006).…”

Section: Photopigment Analysismentioning

confidence: 99%

High plasticity in inorganic carbon uptake by Southern Ocean phytoplankton in response to ambient CO2

Neven

Stefels

Heuven

et al. 2011

Deep Sea Research Part II: Topical Studies in Oceanography

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An optimized method for automated analysis of algal pigments by HPLC

Cited by 89 publications

References 22 publications

Improvement of the Extraction Process for High Commercial Value Pigments fromDesmodesmussp. Microalgae

Improvement of the Extraction Process for High Commercial Value Pigments fromDesmodesmussp. Microalgae

Environmental and nutrient conditions influence fucoxanthin productivity of the marine diatom Phaeodactylum tricornutum grown on palm oil mill effluent

High plasticity in inorganic carbon uptake by Southern Ocean phytoplankton in response to ambient CO2

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