An optimized antibody-chelator conjugate for imaging of carcinoembryonic antigen with indium-111

Sumerdon, Gail A.; Rogers, Patrick E.; Lombardo, Christine M.; Schnobrich, Karen E.; Melvin, Susan L.; Hobart, Edward D.; Tribby, Ilse I. E.; Stroupe, Stephen D.; Johnson, David K.

doi:10.1016/0883-2897(90)90154-s

Cited by 14 publications

(10 citation statements)

References 19 publications

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“…The synthesis of compound 2 will be presented elsewhere. The generation and characterization of the anti-CEA antibody used in these studies has been previously described (15). C110 is an IgGi murine monoclonal antibody that is presently in clinical trials in the form of an indium-ill-labeled conjugate of compound 4 (16).…”

Section: Chart I Ncs Ncsmentioning

confidence: 99%

Characterization of antibody-chelator conjugates: determination of chelator content by terbium fluorescence titration

Brandt

Schnobrich²,

Johnson

1991

Bioconjugate Chem.

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Fluorescence titrations were performed by adding varying mole ratios of terbium(III) to antibody conjugates formed by benzyl isothiocyanate derivatives of three different polyaminopolycarboxylate chelators (NTA, EDTA, and DTPA) and the results compared to values for average chelator content obtained by cobalt-57 binding assays. For two different murine monoclonal antibodies, the average chelator content obtained by terbium fluorescence titration correlated closely with that measured by the cobalt-57 binding assay. It is concluded that lanthanide fluorescence titrations provide a useful alternative to radiometal binding assays for the determination of chelator content in protein-chelator conjugates.

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Section: Chart I Ncs Ncsmentioning

confidence: 99%

Characterization of antibody-chelator conjugates: determination of chelator content by terbium fluorescence titration

Brandt

Schnobrich²,

Johnson

1991

Bioconjugate Chem.

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“…Human/murine chimeric T84.66 minibody (cT84.66 minibody) is an anti-CEA engineered bivalent fragment (V L -linker-V H -C H 3) with high affinity (K a ϭ 4.0 ϫ 10 10 M Ϫ1 ) and specificity to CEA. Details of its production, characterization, purification, and radiolabeling have been reported previously (7)(8)(9)(10)(11). Briefly, preparation of the radiolabeled dose involved incubation of 123 I at a ratio of 5-10 mCi to 1 mg in the presence of Iodogen, followed by purification by size exclusion high-performance liquid chromatography.…”

Section: Methodsmentioning

confidence: 99%

Pilot Trial Evaluating an 123I-Labeled 80-Kilodalton Engineered Anticarcinoembryonic Antigen Antibody Fragment (cT84.66 Minibody) in Patients with Colorectal Cancer

Wong

Chu²,

Williams³

et al. 2004

Clinical Cancer Research

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“…MAb CllO is a murine IgGl reactive with carcinoembryonic antigen (18) and was obtained as a gift from Dr. David Johnson of Abbott Laboratories. Mel-14 is a murine IgG2a reactive with the tumor-associated chondroitin sulfate present in melanomas and gliomas (19).…”

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confidence: 99%

“…Sodium [125I]iodide and sodium [131I]iodide, both in 0.1 N NaOH, were obtained from Du Pont-New England Nuclear (North Billerica, MA). MAb C110 is a murine IgGl reactive with carcinoembryonic antigen (18) and was obtained as a gift from Dr. David Johnson of Abbott Laboratories. Mel-14 is a murine IgG2a reactive with the tumor-associated chondroitin sulfate present in melanomas and gliomas (19).…”

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confidence: 99%

Radioiodination of antibodies via N-succinimidyl 2,4-dimethoxy-3-(trialkylstannyl)benzoates

Vaidyanathan¹,

Zalutsky²

1990

Bioconjugate Chem.

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We have previously shown that use of N-succinimidyl 3-iodobenzoate (SIB) for radioiodination of monoclonal antibodies (MAbs) decreases the loss of radioiodine in vivo compared to MAbs labeled by using conventional methods. Herein, the synthesis of N-succinimidyl 2,4-dimethoxy-3-(trialkylstannyl)benzoates (alkyl = Me, Bu) are described as is their use as precursors for the radiosynthesis of N-succinimidyl 2,4-dimethoxy-3-iodobenzoate (SDMIB). A MAb F(ab')2 fragment labeled with SDMIB retained its ability to bind specifically to tumor homogenates. Paired-label tissue distribution studies indicate that the thyroid uptake (an indicator of deiodination) of hydrolyzed SDMIB was about 20 times that of hydrolyzed SIB. In contrast, thyroid uptake for SDMIB, when conjugated to a MAb, was only 1.4-2.8 times that for SIB and was considerably lower than levels reported in the literature for MAbs labeled by using direct, electrophilic iodination methods. Although MAbs labeled with SDMIB are significantly more inert to dehalogenation than those labeled by conventional methods, compared to the original SIB reagent, addition of two methoxy groups decreased retention of label in vivo.

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An optimized antibody-chelator conjugate for imaging of carcinoembryonic antigen with indium-111

Cited by 14 publications

References 19 publications

Characterization of antibody-chelator conjugates: determination of chelator content by terbium fluorescence titration

Characterization of antibody-chelator conjugates: determination of chelator content by terbium fluorescence titration

Pilot Trial Evaluating an 123I-Labeled 80-Kilodalton Engineered Anticarcinoembryonic Antigen Antibody Fragment (cT84.66 Minibody) in Patients with Colorectal Cancer

Radioiodination of antibodies via N-succinimidyl 2,4-dimethoxy-3-(trialkylstannyl)benzoates

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