An optimistic view for quantifying mRNA in post-mortem human brain

Preece, Paul; Virley, David; Costandi, Moheb; Coombes, Robert; Moss, Stephen J.; Mudge, Anne W.; Jazin, Elena; Cairns, Nigel J.

doi:10.1016/s0169-328x(03)00208-0

Cited by 50 publications

(25 citation statements)

References 30 publications

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“…Beta-actin was used as reference gene for the adjustment of relative expression data [18,19]. We used the QuantumRNA b-actin Internal Standards with Competimer technology (Ambion, Boston, Massachusetts, USA) to solve the problem posed by relative low expression levels of total SNCA 126 as described [18].…”

Section: Methodsmentioning

confidence: 99%

Low alpha-synuclein 126 mRNA levels in dementia with Lewy bodies and Alzheimer disease

Beyer¹,

Humbert

Ferrer

et al. 2006

NeuroReport

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Alpha-synuclein, a main component of Lewy bodies in synucleinopathies and senile plaques in Alzheimer disease, is centrally involved in neurodegeneration. Three different isoforms (alpha-synuclein 112, 126, and 140) resulting from alternative splicing have been described so far. The present study explores alpha-synuclein 126 mRNA expression levels in the prefrontal cortex of six patients with dementia with Lewy bodies, eight patients with Lewy body variant of Alzheimer disease, eight patients with Alzheimer disease, and 10 controls. Relative alpha-synuclein 126 expression levels were determined by real-time polymerase chain reaction with competimer technology. Alpha-synuclein 126 mRNA expression was markedly decreased in the three dementias in comparison with controls, suggesting an important role of this alpha-synuclein isoform in the normal brain.

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Section: Methodsmentioning

confidence: 99%

Low alpha-synuclein 126 mRNA levels in dementia with Lewy bodies and Alzheimer disease

Beyer¹,

Humbert

Ferrer

et al. 2006

NeuroReport

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“…[4]). But recent studies could show that isolation of intact RNA is possible even several days postmortem [55][56][57][58]43,42,45].…”

Section: Mrna Stability In Postmortem Tissuementioning

confidence: 99%

mRNA profiling in forensic genetics I: Possibilities and limitations

Vennemann

Koppelkamm

2010

Forensic Science International

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“…Recently, several authors described successful gene expression analysis from postmortem human brain tissue (e.g., [5][6][7]). Additionally, Bauer et al [8][9][10] reported successful RNA analysis from body fluids in crime scene stains.…”

Section: Introductionmentioning

confidence: 99%

Successful RNA extraction from various human postmortem tissues

et al. 2006

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Recently, several authors described the observation that RNA degradation does not correlate with the postmortem interval (PMI), but rather with other parameters like environmental impact and the circumstances of death. Therefore, the question arose if the analysis of gene expression could be a valuable tool in forensic genetics to contribute to the determination of the cause of death. In our study, six human tissues obtained from six individuals with PMI varying between 15 and 118 h were used for total RNA extraction. Quantification was performed using a GAPDH real-time assay, and the quality of mRNA was checked by amplification of different fragment lengths of the GAPDH transcript. In our set of samples, nearly all tissues in all PMI revealed satisfactory results, while skeletal muscle, followed by brain and heart, gave the best results. No correlation between PMI and RNA degradation could be detected, as very good results were observed for all tissues from the individual with the longest PMI. The highly promising results obtained in this study raise hopes that in the near future several fields of forensic investigation may profit from additional information about gene expression patterns and their correlation with pathological findings.

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An optimistic view for quantifying mRNA in post-mortem human brain

Cited by 50 publications

References 30 publications

Low alpha-synuclein 126 mRNA levels in dementia with Lewy bodies and Alzheimer disease

Low alpha-synuclein 126 mRNA levels in dementia with Lewy bodies and Alzheimer disease

mRNA profiling in forensic genetics I: Possibilities and limitations

Successful RNA extraction from various human postmortem tissues

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