mRNA profiling in forensic genetics I: Possibilities and limitations

Vennemann, Marielle; Koppelkamm, Antje

doi:10.1016/j.forsciint.2010.07.006

Cited by 83 publications

(54 citation statements)

References 69 publications

(88 reference statements)

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“…Therefore, assays targeting eRNA may provide narrower spatiotemporal inferences of organism presence or abundance than eDNA. Forensic scientists have used messenger RNA (mRNA) to identify different body fluids left at crime scenes (Vennemann and Koppelkamm 2010), which suggests mRNA could provide information about the physiological origin of biomaterial in environmental samples. Microbial metatranscriptomics provides guidance for using HTS technologies to characterize eRNA (Shi et al 2009).…”

Section: Isolating Molecules Other Than Dna From Environmental Samplesmentioning

confidence: 99%

The ecology of environmental DNA and implications for conservation genetics

2015

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Environmental DNA (eDNA) refers to the genetic material that can be extracted from bulk environmental samples such as soil, water, and even air. The rapidly expanding study of eDNA has generated unprecedented ability to detect species and conduct genetic analyses for conservation, management, and research, particularly in scenarios where collection of whole organisms is impractical or impossible. While the number of studies demonstrating successful eDNA detection has increased rapidly in recent years, less research has explored the ''ecology'' of eDNA-myriad interactions between extraorganismal genetic material and its environment-and its influence on eDNA detection, quantification, analysis, and application to conservation and research. Here, we outline a framework for understanding the ecology of eDNA, including the origin, state, transport, and fate of extraorganismal genetic material. Using this framework, we review and synthesize the findings of eDNA studies from diverse environments, taxa, and fields of study to highlight important concepts and knowledge gaps in eDNA study and application. Additionally, we identify frontiers of conservation-focused eDNA application where we see the most potential for growth, including the use of eDNA for estimating population size, population genetic and genomic analyses via eDNA, inclusion of other indicator biomolecules such as environmental RNA or proteins, automated sample collection and analysis, and consideration of an expanded array of creative environmental samples. We discuss how a more complete understanding of the ecology of eDNA is integral to advancing these frontiers and maximizing the potential of future eDNA applications in conservation and research.

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Section: Isolating Molecules Other Than Dna From Environmental Samplesmentioning

confidence: 99%

The ecology of environmental DNA and implications for conservation genetics

2015

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“…Various multiplexes have been reported, using reverse transcription endpoint polymerase chain reaction (RT-PCR) methods and real-time quantitative reverse transcriptase-PCR (qRT-PCR) assay, including body fluid-specific markers (43)(44)(45)(46)(47)(48). Since many transcripts are not completely tissue specific but usually show differences in expression level, qRT-PCR might be more appropriate for the detection of relative gene expression levels in different samples, and endpoint PCR might be suitable for the detection of certain transcripts with highly tissue-specific expression (48,49). In 2005, Juusola and Ballantyne (45) proposed a multiplex RT-PCR method for the identification of body fluids that are commonly encountered in forensic casework.…”

Section: Emerging Techniques Messenger Rna Profiling Methodsmentioning

confidence: 99%

Body fluid identification in forensics

et al. 2012

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“…Numerous RNA families have been reported (Vennemann and Koppelkamm, 2010 Oehmichen and Zilles, (1984) first reported the use of RNA in forensic science, describing post-mortem RNA synthesis. It was nearly another decade before a new report of RNA use in this research area was published (Phang, et al, 1994).…”

Section: Ribonucleic Acid (Rna)mentioning

confidence: 99%

“…Bauer, (2007) reported that though most circumstances leading to death do not reveal morphological changes within the cells of a tissue; the functional quality in the cell's mRNA pattern is, however, affected. Consequently, the cell responds rapidly, thus leading to a change in proportions of specific mRNAs due to the effects of external factors on the body (Vennemann, and Koppelkamm, 2010). Different gene expression patterns were observed in various types of death depending on the markers evaluated (Matsuo et al, 2009;Takahashi, 2008).…”

Section: Determination Of Circumstances Leading To Deathmentioning

confidence: 99%