An optimised protocol for plant regeneration from embryogenic suspension cultures of date palm, Phoenix dactylifera L., cv. Deglet Nour

Fki, Lotfi; Masmoudi, R.; Drira, Noureddine; Rival, Alain

doi:10.1007/s00299-002-0558-5

Cited by 107 publications

(44 citation statements)

References 16 publications

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“…Jihel and Bousthami Noir (Zouine and Hadrami, 2007), cv. Deglet Nour (Fki et al, 2003), and cv. Deglet Bay (Othmani et al, 2009) showed that 2,4-D was required for the induction of indirect somatic embryogenesis from leaf explants.…”

Section: Plant Growth Regulators' Effect On Callogenesis and Direct Ementioning

confidence: 99%

“…Date palm leaves are less frequently used than shoot tips in in vitro micropropagation. For callogenesis and rhizogenesis, young leaves from embryogenic suspension cultures (Fki et al, 2003;Othmani et al, 2009), and leaf segments from the seedling plantlet cultures were used (Gueye et al, 2009). Although a few reports on direct embryogenesis (Hegazy, 2008;Hegazy and Aboshama, 2010;Sidky and Zaid, 2012) from shoot tip explants in date palm have been published, no reports on the direct embryogenesis from adult leaf explants have been published thus far.…”

Section: Introductionmentioning

confidence: 99%

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Induction of direct somatic embryogenesis and callogenesis in date palm (Phoenix dactylifera L.) using leaf explants

Baharan¹,

Mohammadi²

2018

bta

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An efficient method for direct somatic embryogenesis and callogenesis from date palm (Phoenix dactylifera L. cv. Estamaran) was established using leaf explants. The effect of pretreating leaf explants with three antioxidant combinations on the browning was studied. The results showed that both the pre-treatments were effective against the browning of explants. Surface sterilization of explants, without pre-treatment, enhanced the browning. After recognizing the importance of antioxidants, we cultured the explants on an MS medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) at 0.1, 0.5, 1, 2, and 5 mg/l, thiadiazuron (TDZ) at 0 and 5 mg/l and 6-benzyladeninepurine (BAP) at 0, 5, and 10 mg/l. Callogenesis and direct embryogenesis on the surface of the leaf explants were observed. Only on the medium containing 5 mg/l 2,4-D, 10 mg/l BAP, and 5 mg/l TDZ globular embryos were formed directly on the leaf explants. The percentage of callus formation increased with an increase in the 2,4-D concentration; however, high concentrations of cytokinin caused a reduction of callogenesis. A medium supplemented with 5 mg/l 2,4-D, 5 mg/l TDZ, and 5 mg/l BAP, and 2 mg/l 2,4-D, and 5 mg/l BAP produced the highest number of calli per replication (2.7) on the leaf surfaces and leaf margins. Along with the advantages of direct somatic embryogenesis, this protocol facilitated the prospect of micropropagation without resorting to the intermediary callus stage, an important aspect to commercial production, and without sacrificing the plant by excising the shoot tips, which are commonly used for date palm tissue cultures.

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Section: Plant Growth Regulators' Effect On Callogenesis and Direct Ementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Induction of direct somatic embryogenesis and callogenesis in date palm (Phoenix dactylifera L.) using leaf explants

Baharan¹,

Mohammadi²

2018

bta

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“…Exogenous auxins and cytokinins are the main plant growth regulators (PGRs) involved in the control of cell division and differentiation [12]. The role of these PGRs in the regeneration performance of date palm has been previously described [8–10]. …”

Section: Introductionmentioning

confidence: 99%

Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactyliferaL.) Sahelian Cultivars

Sané

Aberlenc-Bertossi

Diatta

et al. 2012

The Scientific World Journal

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This study provides a physiological analysis of somatic embryogenesis in four elite cultivars of date palms: Ahmar, Amsekhsi, Tijib, and Amaside, from the initial callogenesis to establishment and proliferation of embryogenic suspension cultures. Somatic embryos development and in vitro plants rooting were also studied. For each step, auxins and cytokinins concentrations were optimised. The primary callogenesis from leaf explants of seedlings appeared highly dependent on genotype. Ahmar (80%) and Amsekhsi (76%) appeared highly callogenic, whereas Tijib (10%) and Amaside (2%) produced low amounts of calluses. 2,4-Dichlorophenoxyacetic acid appeared favorable to the induction of primary callogenesis and its effect was enhanced by the addition of benzyl adenine or adenine sulfate. Secondary friable calli obtained from chopped granular calli were used to initiate embryogenic cell suspensions in media supplied with 2,4-dichlorophenoxyacetic acid. Suspension cultures showed a growth rate of fourfold after four subcultures in presence of 2,4-dichlorophenoxyacetic acid 2 mg/L. Our results showed that a seven-day transitory treatment with benzyl adenine 0,5 mg/L was necessary to optimize embryos development. Naphthalene acetic acid induced the development of primary orthogravitropic roots during embryos germination. The comparison with cytofluorometry of nuclear DNA amounts showed no significant difference in ploidy level between regenerated plants and seedlings.

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“…Hence, in vitro propagation is the only available alternative tool to produce disease free, uniform and good quality planting material to establish large scale cultivation within a short period of time. Date palm is mainly in vitro multiplied through somatic embryogenesis (Fki et al, 2003;Al-Khateeb, 2008a;Othmani et al, 2009). Most of the protocols for somatic embryogenesis of date palm made the use of high concentrations of 2, 4-dichlorophenoxy acetic acid (2, 4-D) in media which is known to be associated with genetic instability in regenerated plants.…”

Section: Introductionmentioning

confidence: 99%

Micro propagation of Date Palm (Phoenix dactylifera L.) var. Zaghlol via Direct Organogenesis

Baghdady

2018

Annals of Agricultural Science, Moshtohor

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This study was an attempt to develop an in vitro protocol for propagation date palm (Phoenix dactylifera L.) Zaghlol cv via direct shoot regeneration system. Combinations between plant growth regulators were used in order to achieve this goal. Shoot tips were cultured on MS medium supplemented with cytokinins namely BA or KIN at different concentrations either individually or in combinations with NAA at 0.0, 1.0, 2.0 and 4.0 mg /L in the presence of 1.0 mg/ L NOA. Results revealed that MS medium supplemented with BA or KIN at 1.0 mg /Lin combination with NAA at 1.0 mg/ L was the best for bud formation from shoot tip after 8 weeks of culturing at initiation stage. Sub-culturing the formed buds on solid MS medium supplemented with 1.0 mg/ L of BA and KIN gave the maximum number of shoots with appropriate length at multiplication stage. MS medium supplemented with 10 mg/ L of GA3 caused the maximum shoot length at elongation stage compared with control and other treatments. Moreover, maximum rooting percentage, root number and root length were obtained as MS medium supplemented with 0.5 mg/L IBA and 1.0 mg/L NAA was used.

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An optimised protocol for plant regeneration from embryogenic suspension cultures of date palm, Phoenix dactylifera L., cv. Deglet Nour

Cited by 107 publications

References 16 publications

Induction of direct somatic embryogenesis and callogenesis in date palm (Phoenix dactylifera L.) using leaf explants

Induction of direct somatic embryogenesis and callogenesis in date palm (Phoenix dactylifera L.) using leaf explants

Influence of Growth Regulators on Callogenesis and Somatic Embryo Development in Date Palm (Phoenix dactyliferaL.) Sahelian Cultivars

Micro propagation of Date Palm (Phoenix dactylifera L.) var. Zaghlol via Direct Organogenesis

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