An on-site, highly specific immunosensor for Escherichia coli detection in field milk samples from mastitis-affected dairy cattle

Zhang, Jian; Jiang, Yanting; Xia, Xin; Cheng, Cheng; Almeida, Ricardo Augusto Monteiro de Barros; Eda, Shigetoshi; Qi, Haochen

doi:10.1016/j.bios.2020.112366

Cited by 50 publications

(19 citation statements)

References 39 publications

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“…Often, it is caused by the invasion and proliferation of pathogenic microorganisms, physical damage, chemical stimulation and the cow's immune response (1,2,37). However, among these causes, E. coli is the most common culprit (38,39). When E. coli successfully penetrate the mammary gland through the physical barrier of the teat end, it causes an inflammatory response in the mammary gland (38,40).…”

Section: Discussionmentioning

confidence: 99%

Differential Expression Profiles of lncRNA Following LPS-Induced Inflammation in Bovine Mammary Epithelial Cells

Wang

Yang

et al. 2021

Front. Vet. Sci.

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Bovine mastitis is an inflammatory response of mammary glands caused by pathogenic microorganisms such as Escherichia coli (E. coli). As a key virulence factor of E. coli, lipopolysaccharide (LPS) triggers innate immune responses via activation of the toll-like-receptor 4 (TLR4) signaling pathway. However, the molecular regulatory network of LPS-induced bovine mastitis has yet to be fully mapped. In this study, bovine mammary epithelial cell lines MAC-T were exposed to LPS for 0, 6 and 12 h to assess the expression profiles of long non-coding RNAs (lncRNAs) using RNA-seq. Differentially expressed lncRNAs (DElncRNAs) were filtered out of the raw data for subsequent analyses. A total of 2,257 lncRNAs, including 210 annotated and 2047 novel lncRNAs were detected in all samples. A large proportion of lncRNAs were present in a high abundance, and 112 DElncRNAs were screened out at different time points. Compared with 0 h, there were 22 up- and 25 down-regulated lncRNAs in the 6 h of post-infection (hpi) group, and 27 up- and 22 down-regulated lncRNAs in the 12 hpi group. Compared with the 6 hpi group, 32 lncRNAs were up-regulated and 25 lncRNAs were down-regulated in the 12 hpi group. These DElncRNAs are involved in the regulation of a variety of immune-related processes including inflammatory responses bMECs exposed to LPS. Furthermore, lncRNA TCONS_00039271 and TCONS_00139850 were respectively significance down- and up-regulated, and their target genes involve in regulating inflammation-related signaling pathways (i.e.,Notch, NF-κB, MAPK, PI3K-Akt and mTOR signaling pathway), thereby regulating the occurrence and development of E. coli mastitis. This study provides a resource for lncRNA research on the molecular regulation of bovine mastitis

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Section: Discussionmentioning

confidence: 99%

Differential Expression Profiles of lncRNA Following LPS-Induced Inflammation in Bovine Mammary Epithelial Cells

Wang

Yang

et al. 2021

Front. Vet. Sci.

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“…The change of normalized capacitance per minute, i.e., dC/dt (%/min), directly reflects the S-protein adsorption level, by which the S-protein concentration in solution can be indicated. Using an IDME of micron scale, dC/dt is a competitive parameter to reflect tiny change at the electrode interface [30,37,43]. Therefore, ultra-trace S-protein detection can be expected.…”

Section: Methodsmentioning

confidence: 99%

“…Based on the good specificity and versatile sensing mechanisms, bio-probe based sensors for virus detection are becoming more practicable in recent years as promising approaches [19][20][21]. Also due to the high specificity, reproducibility, and stability, antibody based detections of causative agent are continually adopted in food safety detection [29,30].…”

mentioning

confidence: 99%

Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

Qi¹,

Zhang

Cheng³

et al. 2020

Preprint

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“…Based on the good specificity and versatile sensing mechanisms, bio-probe-based sensors for virus detection are becoming more practicable in recent years as promising approaches 19 – 21 . Also, due to the high specificity, reproducibility, and stability, antibody-based detections of causative agents are continually adopted in food safety detection 22 , 23 .…”

Section: Introductionmentioning

confidence: 99%

Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

et al. 2021

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Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10−6 ng/mL in 20 s, with a wide linear range of 10−5–10−1 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.

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An on-site, highly specific immunosensor for Escherichia coli detection in field milk samples from mastitis-affected dairy cattle

Cited by 50 publications

References 39 publications

Differential Expression Profiles of lncRNA Following LPS-Induced Inflammation in Bovine Mammary Epithelial Cells

Differential Expression Profiles of lncRNA Following LPS-Induced Inflammation in Bovine Mammary Epithelial Cells

Real-time Sensing of Trace Biomarkers from Viruses with a Microfluidic Immunosensor: A Case Study of SARS-CoV-2 Detection in Cold-chain Food

Real-time, selective, and low-cost detection of trace level SARS-CoV-2 spike-protein for cold-chain food quarantine

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