An NO-responsive probe for detecting acute inflammation using NIR-II fluorescence/optoacoustic imaging

Abstract

If left unregulated, acute inflammation could quickly turn into critical conditions with serious complications; however, if it is detected and controlled at an early stage, timely and effective measures could...

A NIR‐II Photoacoustic Probe for High Spatial Quantitative Imaging of Tumor Nitric Oxide in Vivo

“…Due to the synergy of the push-pull and conjugation effects, the λ abs of SPA NO 1 is red-shifted to 1010 nm. After reacting with NO, N-methylaniline undergoes nitrosation, [21][22]29] which inhibited the intramolecular charge transfer effect, thereby hyposochromic-shifting the λ abs to 950 nm. As a result, the ratiometric response of SPA NO 1 to NO can be achieved through the change of the absorption wavelength before and after the probe reacts with NO.…”

“…PA imaging has been shown immense potential for high‐resolution imaging and quantification of NO in vivo. Numbers of PA probes for NO imaging with absorption maximum ( λ abs ) in the near‐infrared‐I window (NIR‐I, 700–900 nm) have been reported recently, and have been initially applied in the fields of cancer diagnosis and treatment [21–25] . Unfortunately, the absorption spectra of some endogenous biomolecules (e.g., hemoglobin, lipids, and melanin) overlap significantly with those of the NIR‐I PA probes, which makes it difficult to avoid the interference of the background signals [26] .…”

“…Numbers of PA probes for NO imaging with absorption maximum (λ abs ) in the near-infrared-I window (NIR-I, 700-900 nm) have been reported recently, and have been initially applied in the fields of cancer diagnosis and treatment. [21][22][23][24][25] Unfortunately, the absorption spectra of some endogenous biomolecules (e.g., hemoglobin, lipids, and melanin) overlap significantly with those of the NIR-I PA probes, which makes it difficult to avoid the interference of the background signals. [26] This obstacle can be effectively overcome by designing ratiometric PA probes, [17,20] for example, our recently developed ratiometric PA probes (PA NO 2) enables high-resolution imaging of NO in the mouse brain with micron-scale resolution.…”

A near-infrared fluorescent probe for detection of H2S and its application in monitoring meat freshness and plant growth under aluminium-induced stress

“…Fluorescent probes have gained widespread application in various areas such as food examination, biological imaging, and environmental monitoring due to their high selectivity, non-destructive analysis, and excellent compatibility. 5–13 Several mechanisms for designing fluorescent H 2 S probes have been successfully created, including H 2 S-mediated reductions of azides or nitro groups to amino groups, copper sulfide precipitation reactions, and the combination of nucleophilic reactions and thiolysis of leaving groups. 3,14,15 In particular, the last approach, which was developed by Feng and coworkers, 15 exhibits fast response and high sensitivity.…”

Visualizing Efficacy of Antineoplastic Drug via Ratiometric Near-Infrared Fluorescence Imaging of Tumor-Associated Macrophage-Specific Nitric Oxide

“…The fluorescence imaging technique enables noninvasive, real-time, dynamic, precise, and sensitive detection of bioactive molecules. − Thus, plentiful strategies for fluorescence imaging of NO have been carried out. − Among them, near-infrared (NIR) fluorescence imaging methods are powerful means currently applied in drug efficacy evaluation in deep tissue. Previous works have reported several NIR probes with excellent performances for NO detection .…”