Visualizing Efficacy of Antineoplastic Drug via Ratiometric Near-Infrared Fluorescence Imaging of Tumor-Associated Macrophage-Specific Nitric Oxide

Wu, Chuanchen; Zhang, Fanghui; Mao, Yuantao; Qi, Xinru; Wang, Xin; Zhang, Wen; Tang, Bo; Li, Ping

doi:10.1021/cbmi.3c00016

“…[ 105 ] And the level of NO in TAM can also be detected for the evaluation of anticancer drug efficacy. [ 106 ] Chunfu Zhang et al constructed a Pd/Pt/Au tri‐metallic mesoporous nanoreactor shrouded with tannic acid iron ion (Fe III TA) network (PdPtAu@TF), which controllably degraded and accumulated in acidified surroundings and exhibited notable catalytic activities such as peroxidase, catalase, and glucose oxidase, etc. Thus, it can simultaneously achieve the ferroptosis sensitization and TAM repolarization through a series of metabolic reprogramming reactions.…”

Section: Methods To Reconstruct Tmementioning

confidence: 99%

Enhancing the Treating Efficacy of Immunotherapy through the Restructure of Tumor Microenvironment

Gong,

Jia,

Zhou

et al. 2023

Advanced NanoBiomed Research

0

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The therapeutic modes of cancers have been profoundly renovated by immunotherapies, which have shown extraordinary treating efficacy in certain tumor entities. However, the majority of cancer patients have not profited from it because of the negative effects of tumor microenvironment (TME) on human innate and/or adaptive immunity, including hypoxia, acidification, irregular vasculature, and a plethora of immunosuppressive cells and small molecules, which contribute to tumor progression, migration, resistance to drug, and so forth. Accordingly, it is feasible to enhance the efficacy of immunotherapies and increase the patients’ survival through the restructure of TME. Herein, the mechanisms and reverberations of aforementioned immunosuppressive elements are concentrated on, and latest therapeutic achievements and combined technologies that have been demonstrated effective in boosting immunotherapies by TME modulation are enumerated.

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Ratiometric Afterglow Luminescent Imaging of Matrix Metalloproteinase‐2 Activity via an Energy Diversion Process

Huang,

Zeng,

Huang

et al. 2024

Angewandte Chemie

0

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Ratiometric afterglow luminescent (AGL) probes are attractive for in vivo imaging due to their high sensitivity and signal self‐calibration function. However, there are currently few ratiometric AGL probes available for imaging enzymatic activity in living organisms. Here, we present an energy diversion (ED) strategy that enables the design of an enzyme‐activated ratiometric AGL probe (RAG‐RGD) for in vivo afterglow imaging. The ED process provides RAG‐RGD with a radiative transition for an ‘always on’ 520‐nm AGL signal (AGL520) and a cascade three‐step energy transfer (ET) process for an ‘off‐on’ 710‐nm AGL signal (AGL710) in response to a specific enzyme. Using matrix metalloproteinase‐2 (MMP‐2) as an example, RAG‐RGD shows a significant ~11‐fold increase in AGL710/AGL520 toward MMP‐2. This can sensitively detect U87MG brain tumors through ratiometric afterglow imaging of MMP‐2 activity, with a high signal‐to‐background ratio and deep imaging depth. Furthermore, by utilizing the self‐calibration effect of ratiometric imaging, RAG‐RGD demonstrated a strong negative correlation between the AGL710/AGL520 value and the size of orthotopic U87MG tumor, enabling accurate monitoring of orthotopic glioma growth in vivo. This ED process may be applied for the design of other enzyme‐activated ratiometric afterglow probes for sensitive afterglow imaging.

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Ratiometric Afterglow Luminescent Imaging of Matrix Metalloproteinase‐2 Activity via an Energy Diversion Process

Huang,

Zeng,

Huang

et al. 2024

Angew Chem Int Ed

0

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Ratiometric afterglow luminescent (AGL) probes are attractive for in vivo imaging due to their high sensitivity and signal self‐calibration function. However, there are currently few ratiometric AGL probes available for imaging enzymatic activity in living organisms. Here, we present an energy diversion (ED) strategy that enables the design of an enzyme‐activated ratiometric AGL probe (RAG‐RGD) for in vivo afterglow imaging. The ED process provides RAG‐RGD with a radiative transition for an ‘always on’ 520‐nm AGL signal (AGL520) and a cascade three‐step energy transfer (ET) process for an ‘off‐on’ 710‐nm AGL signal (AGL710) in response to a specific enzyme. Using matrix metalloproteinase‐2 (MMP‐2) as an example, RAG‐RGD shows a significant ~11‐fold increase in AGL710/AGL520 toward MMP‐2. This can sensitively detect U87MG brain tumors through ratiometric afterglow imaging of MMP‐2 activity, with a high signal‐to‐background ratio and deep imaging depth. Furthermore, by utilizing the self‐calibration effect of ratiometric imaging, RAG‐RGD demonstrated a strong negative correlation between the AGL710/AGL520 value and the size of orthotopic U87MG tumor, enabling accurate monitoring of orthotopic glioma growth in vivo. This ED process may be applied for the design of other enzyme‐activated ratiometric afterglow probes for sensitive afterglow imaging.

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Visualizing Efficacy of Antineoplastic Drug via Ratiometric Near-Infrared Fluorescence Imaging of Tumor-Associated Macrophage-Specific Nitric Oxide

Cited by 5 publications

References 28 publications

Enhancing the Treating Efficacy of Immunotherapy through the Restructure of Tumor Microenvironment

Enhancing the Treating Efficacy of Immunotherapy through the Restructure of Tumor Microenvironment

Ratiometric Afterglow Luminescent Imaging of Matrix Metalloproteinase‐2 Activity via an Energy Diversion Process

Ratiometric Afterglow Luminescent Imaging of Matrix Metalloproteinase‐2 Activity via an Energy Diversion Process

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