An mTOR siRNA‐Loaded Spermidine/DNA Tetrahedron Nanoplatform with a Synergistic Anti‐Inflammatory Effect on Acute Lung Injury

Abstract

Acute lung injury (ALI) is characterized by severe inflammation and damage to the lung air–blood barrier, resulting in respiratory function damage and life‐threatening outcomes. Macrophage polarization plays an essential role in the occurrence, development, and outcome of ALI. As drug carriers, self‐assembled DNA nanostructures can potentially overcome the drawbacks and limitations of traditional anti‐inflammatory agents owing to their nontoxicity, programmability, and excellent structural control at the nanoscale. A small interfering RNA (siRNA) and drug dual therapy nanoplatform are proposed and constructed here to combat ALI. The nanoplatform consists of a spermidine‐assembled DNA tetrahedron and four mammalian target of rapamycin siRNAs. Spermidine serves as a mediator of drug delivery vehicle synthesis and a drug that alters macrophage polarization. Both spermidine and siRNA exert anti‐inflammatory effects in vitro and in vivo by regulating the macrophage phenotype. More importantly, these factors exhibit a synergistic anti‐inflammatory effect by promoting macrophage autophagy. For the first time, an anti‐inflammatory dual therapy strategy that uses self‐assembled DNA nanostructures as nontoxic, programmable delivery vehicles is proposed and demonstrated through this work. Future work on utilizing DNA nanostructures for the treatment of noncancerous diseases such as ALI is highly promising and desirable.

p65 siRNA-Loaded Buformin/DNA Nanoprisms Alleviate Acute Lung Injury through Inhibiting NLRP3-Mediated Pyroptosis

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Reverse Engineering of DNA and RNA Hybrid Origami Structures as Targeted Nanomedicine for KRAS-Mutated Lung Cancer Therapy

“…Refer to the previous report for the detailed steps. 18 Briefly, 40 μg of protein samples from different drug treated groups was separated by 12% SDS-PAGE (KRAS, GAPDH) and incubated with the appropriate antibody. The image was captured by using a chemiluminescence detection system.…”

“…The tumors were sliced and homogenized by grinding. Refer to the previous report for the detailed steps . Briefly, 40 μg of protein samples from different drug treated groups was separated by 12% SDS-PAGE (KRAS, GAPDH) and incubated with the appropriate antibody.…”

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