An MR-compatible device for the in situ assessment of isometric contractile performance of mouse hind-limb ankle flexors

Drost, Maarten R.; Heemskerk, Anneriet M.; Strijkers, Gustav J.; Dekkers, E.C.A.; Kranenburg, Guido; Nicolay, Klaas

doi:10.1007/s00424-003-1181-1

Cited by 8 publications

(8 citation statements)

References 8 publications

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“…where B 0 =~0.2-0.4 mT) demonstrated identical slopes with a difference for the Y-intercepts between the two testing conditions. In agreement with a previous study (11), these findings indicated that the magnetic field led to changes in the force offset, reaching~100 mN, that did not influence the reliability of the measured force production. Additionally, when considering the ergometer as a whole (i.e.…”

Section: Mechanical Performance Of the Ergometersupporting

confidence: 92%

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High‐field (11.75T) multimodal MR imaging of exercising hindlimb mouse muscles using a non‐invasive combined stimulation and force measurement device

et al. 2014

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We have designed and constructed an experimental set-up allowing electrical stimulation of hindlimb mouse muscles and the corresponding force measurements at high-field (11.75T). We performed high-resolution multimodal MRI (including T2 -weighted imaging, angiography and diffusion) and analysed the corresponding MRI changes in response to a stimulation protocol. Mice were tested twice over a 1-week period to investigate the reliability of mechanical measurements and T2 changes associated with the stimulation protocol. Additionally, angiographic images were obtained before and immediately after the stimulation protocol. Finally, multislice diffusion imaging was performed before, during and immediately after the stimulation session. Apparent diffusion coefficient (ADC) maps were calculated on the basis of diffusion weighted images (DWI). Both force production and T2 values were highly reproducible as illustrated by the low coefficient of variation (<8%) and high intraclass correlation coefficient (≥0.75) values. Maximum intensity projection angiographic images clearly showed a strong vascular effect resulting from the stimulation protocol. Although a motion sensitive imaging sequence was used (echo planar imaging) and in spite of the strong muscle contractions, motion artifacts were minimal for DWI recorded under exercising conditions, thereby underlining the robustness of the measurements. Mean ADC values increased under exercising conditions and were higher during the recovery period as compared with the corresponding control values. The proposed experimental approach demonstrates accurate high-field multimodal MRI muscle investigations at a preclinical level which is of interest for monitoring the severity and/or the progression of neuromuscular diseases but also for assessing the efficacy of potential therapeutic interventions.

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Section: Mechanical Performance Of the Ergometersupporting

confidence: 92%

“…r 2 > 0.99) with a very slight hysteresis (i.e. ~2%) which is in line with a previous device compatible with a high‐field MR system . In order to further illustrate the robustness of our ergometer, we performed two sets of force measurements during a standardized stimulation protocol over a 1‐week period.…”

Section: Discussionsupporting

confidence: 81%

High‐field (11.75T) multimodal MR imaging of exercising hindlimb mouse muscles using a non‐invasive combined stimulation and force measurement device

et al. 2014

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“…Experiments were performed on female C57BL/6 mice (23 ± 2 g; mean ± SD) that were positioned in an MRI‐compatible cradle equipped with a torque transducer (24). The mice were anesthetized with isoflurane (Schering‐Plough, Animal Health, Maarssen, The Netherlands), 1.0–1.5% in air, with a flow rate of 0.4 l/min, controlled by a flow meter system (UNO, Zevenaar, The Netherlands).…”

Section: Methodsmentioning

confidence: 99%

DTI‐based assessment of ischemia‐reperfusion in mouse skeletal muscle

Heemskerk¹,

Drost²,

Bochove³

et al. 2006

Magnetic Resonance in Med

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Diffusion tensor imaging (DTI) is frequently applied to characterize the microscopic geometrical properties of tissue. To establish whether and how diffusion MRI responds to transient ischemia of skeletal muscle, we studied the effects of ischemia and reperfusion using DTI and T 2 -weighted MRI before and during ischemia and up to 24 hr after reperfusion. Ischemia was induced by 50 min of hindlimb occlusion with or without dorsal flexor stimulation. During ischemia the apparent diffusion coefficient (ADC) tended to decrease (up to 15%), whereas the fractional anisotropy (FA) and T 2 showed a varied response depending on the protocol and muscle type. During reperfusion the ADC and T 2 initially increased and subsequently renormalized for the occlusion protocol. For the occlusion plus stimulation (OS) protocol, the FA was decreased by 13% and the ADC and T 2 were increased by 20% and 57%, respectively, after 24 hr in the stimulated muscle complex. In the latter tissue the three DTI eigenvalues gradually increased upon reperfusion. The smallest eigenvalue (

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“…Experiments ( N = 6) were performed on male C57BL/6 mice (26.5 ± 1.4 g, mean ± SD) that were positioned in an MRI‐compatible device (19). The mice were anesthetized with isoflurane (Schering‐Plough, Animal Health, Maarssen, The Netherlands), 1.0–1.5% in air, with a flow rate of 0.4 l/min, controlled by a flow meter system (UNO, Zevenaar, The Netherlands).…”

Section: Methodsmentioning

confidence: 99%

Determination of mouse skeletal muscle architecture using three‐dimensional diffusion tensor imaging

Heemskerk

Strijkers

Vilanova

et al. 2005

Magnetic Resonance in Med

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140

142

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Muscle architecture is the main determinant of the mechanical behavior of skeletal muscles. This study explored the feasibility of diffusion tensor imaging (DTI) and fiber tracking to noninvasively determine the in vivo three-dimensional (3D) architecture of skeletal muscle in mouse hind leg. In six mice, the hindlimb was imaged with a diffusion-weighted (DW) 3D fast spin-echo (FSE) sequence followed by the acquisition of an exerciseinduced, T 2 -enhanced data set. The data showed the expected fiber organization, from which the physiological cross-sectional area (PCSA), fiber length, and pennation angle for the tibialis anterior (

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An MR-compatible device for the in situ assessment of isometric contractile performance of mouse hind-limb ankle flexors

Cited by 8 publications

References 8 publications

High‐field (11.75T) multimodal MR imaging of exercising hindlimb mouse muscles using a non‐invasive combined stimulation and force measurement device

High‐field (11.75T) multimodal MR imaging of exercising hindlimb mouse muscles using a non‐invasive combined stimulation and force measurement device

DTI‐based assessment of ischemia‐reperfusion in mouse skeletal muscle

Determination of mouse skeletal muscle architecture using three‐dimensional diffusion tensor imaging

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