DTI‐based assessment of ischemia‐reperfusion in mouse skeletal muscle

Heemskerk, Anneriet M.; Drost, Maarten R.; Bochove, Glenda S. van; Oosterhout, Matthijs F.M. van; Nicolay, Klaas; Strijkers, Gustav J.

doi:10.1002/mrm.20953

Cited by 88 publications

(106 citation statements)

References 42 publications

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“…Recently, DTI has been used to determine the characteristics of normal and abnormal muscles in both humans and animals. [12][13][14][15][16][17][18] In this study, DTI as an imaging tool was used to evaluate human inflammatory myopathies, which to our knowledge has not been reported before.…”

Section: Resultsmentioning

confidence: 99%

Diffusion tensor imaging in evaluation of thigh muscles in patients with polymyositis and dermatomyositis

Ai¹,

Yu²,

Gao³

et al. 2014

BJR

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Objective: To explore the diffusion tensor imaging (DTI) characteristics of thigh muscles in patients with polymyositis (PM) and dermatomyositis (DM). Methods: 12 patients with known PM/DM and 10 healthy volunteers were enrolled in this study. Both DTI and conventional MR sequences were performed on both thighs of all subjects. Apparent diffusion coefficient (ADC), fractional anisotropy (FA) and three eigenvalues were compared between the PM/DM group and the healthy group. One-way analysis of variance and Student's t-test were used for statistical analyses with a significance of p , 0.05. Results: In the healthy group, the vastus intermedius muscle showed the highest ADC value and the gracilis (GA) muscle showed the lowest ADC value. These results were statistically significant when compared with other muscles (p , 0.05). The GA, semi-tendinosus and semimembranosus muscles showed higher FA values than the other three thigh muscles (p , 0.05). The mean ADC value and three eigenvalues of oedematous muscles in the PM/DM group were higher on average and showed a statistically significant difference when compared with unaffected (non-oedematous muscles in patients) and normal muscles ( p , 0.05). There was no statistical difference in the mean FA value between oedematous and normal muscles. The mean ADC, FA and three eigenvalues in unaffected muscles (in patients) showed no statistical differences from those in normal muscles ( p . 0.05). Conclusion: DTI can be used to quantitatively evaluate the anisotropic diffusion characteristics of muscles in patients with PM/DM. Advances in knowledge: A new application of DTI is proposed for inflammatory myopathies. The results show that ADC and the three eigenvalues were significantly different between diseased and normal muscles, a finding of potential value in both diagnosis and treatment monitoring of myopathies.Polymyositis (PM) and dermatomyositis (DM) are two common idiopathic inflammatory myopathies. In the past, the diagnosis of PM/DM depended primarily on clinical features, enzyme levels within the blood, electromyogram and muscle biopsies.1-3 Recently, MRI has been proven to be valuable in evaluating patients with PM/DM. 4-6 Using traditional sequences, MRI is able to detect muscle oedema and fat infiltration/fatty replacement in muscles, with high spatial resolution and superior soft-tissue contrast compared with other imaging modalities. [7][8][9] However, conventional MR techniques, such as T 2 weighted imaging and short tau inversion recovery, are limited to providing qualitative information, as opposed to quantitative information, which is important for the early detection of inflammatory changes in these diseases. The findings of PM/DM on traditional MRI images are non-specific, making it difficult to distinguish PM/DM from other myopathies on MRI. In addition, the muscular microstructure needs to be evaluated for staging and therapy monitoring in clinical practice.Diffusion tensor imaging (DTI) is an advanced functional MRI technique that has been used to eval...

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Section: Resultsmentioning

confidence: 99%

Diffusion tensor imaging in evaluation of thigh muscles in patients with polymyositis and dermatomyositis

Ai¹,

Yu²,

Gao³

et al. 2014

BJR

View full text Add to dashboard Cite

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“…Irrespective of the anatomical and/or physiological basis of the second and third DTI eigenvectors and eigenvalues, l 2 and l 3 can be expected to be more sensitive to alterations in the dimensions, the shape and the integrity of the skeletal muscle fibers than the principal eigenvalue l 1 . Indeed, Heemskerk et al (166) have recently obtained evidence that, among the DTI eigenvalues, l 3 shows the largest relative response to muscle injury. Figure 8 depicts the time course of l 1 , l 2 and l 3 in mouse tibialis anterior muscle before, during and after a period of severe ischemic stress.…”

Section: Diffusion Mri Of Fiber Architecture and Myocyte Integritymentioning

confidence: 99%

Dynamic MRS and MRI of skeletal muscle function and biomechanics

et al. 2006

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MR is a powerful technique for studying the biomechanical and functional properties of skeletal muscle in vivo in health and disease. This review focuses on 31 P, 1 H and 13 C MR spectroscopy for assessment of the dynamics of muscle metabolism and on dynamic 1 H MRI methods for non-invasive measurement of the biomechanical and functional properties of skeletal muscle. The information thus obtained ranges from the microscopic level of the metabolism of the myocyte to the macroscopic level of the contractile function of muscle complexes. The MR technology presented plays a vital role in achieving a better understanding of many basic aspects of muscle function, including the regulation of mitochondrial activity and the intricate interplay between muscle fiber organization and contractile function. In addition, these tools are increasingly being employed to establish novel diagnostic procedures as well as to monitor the effects of therapeutic and lifestyle interventions for muscle disorders that have an increasing impact in modern society.

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“…Based on the restriction of water diffusion by cell membranes and other subcellular structures and via measuring the intensity of water diffusion in three orthogonal directions, including the primary (l 1 ), secondary (l 2 ) and tertiary (l 3 ) eigenvalues, DTI allows the mapping of the diffusion characteristic of water molecules of biological tissues in vivo. From the previous studies, DTI has been successfully used to non-invasively investigate the diffusive characteristics and microstructure changes of muscular tissues, such as the diffusive properties of the extraocular muscle, 12 the effect of blood flow obstruction and reperfusion, 13 skeleton muscle injury 14,15 and changes after exercise. 16,17 The results demonstrated that there was a geometric correlation between the eigenvalues and muscle fibre architecture.…”

Section: Introductionmentioning

confidence: 99%

In vivomorphological and functional evaluation of the lateral pterygoid muscle: a diffusion tensor imaging study

Liu¹,

Wang²,

Ai³

et al. 2016

BJR

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Objective: To explore the feasibility of morphological and functional evaluation of the lateral pterygoid muscle (LPM) by diffusion tensor imaging (DTI) in vivo. Methods: 30 healthy volunteers underwent DTI with the jaw in the rest position, opening and clenching. Diffusion parameters of the superior head of the LPM (SHLP) and the inferior head of the LPM (IHLP) at different jaw positions were calculated. Results: When the jaw was in the rest position, l 3 of the SHLP was significantly lower than that of the IHLP; fractional anisotropy (FA) value of the SHLP was significant higher than that of the IHLP. There was no significant difference in l 1 , l 2 and apparent diffusion coefficient (ADC) value. During jaw opening, there was significant increase of all three eigenvalues and ADC value, and significant decrease of FA value both at the SHLP and IHLP. Clenching caused a significant increase in the ADC and all three eigenvalues, and caused a significant decrease of FA at the SHLP. However, at the IHLP, the variations of all diffusion parameters by clenching in the intercuspal position showed no significance when compared with those at rest. Conclusion: The morphological and functional changes of LPM fibres caused by jaw movements could be sensitively detected by DTI which may serve as a new and noninvasive method for simultaneously investigating the functional and morphological features of the LPM during jaw movement. Advances in knowledge: A new application of DTI is proposed for the morphological and functional evaluation of the LPMs. The results show that the significant change of three eigenvalues indicates the activity of the LPM in a specific jaw movement, a finding that shows the potential value of DTI serving as a new and non-invasive method for investigation of the LPM.

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DTI‐based assessment of ischemia‐reperfusion in mouse skeletal muscle

Cited by 88 publications

References 42 publications

Diffusion tensor imaging in evaluation of thigh muscles in patients with polymyositis and dermatomyositis

Diffusion tensor imaging in evaluation of thigh muscles in patients with polymyositis and dermatomyositis

Dynamic MRS and MRI of skeletal muscle function and biomechanics

In vivomorphological and functional evaluation of the lateral pterygoid muscle: a diffusion tensor imaging study

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