An MCIA-like complex is required for mitochondrial complex I assembly and seed development in maize

Wang, Gang; Wang, Yongyan; Ni, Jiacheng; Li, Rongrong; Zhu, Fengling; Wang, Ruyin; Tian, Qiuzhen; Shen, Qingwen; Yang, Qinghua; Tang, Jihua; Murcha, Monika W.; Wang, Guifeng

doi:10.1016/j.molp.2022.08.001

Cited by 5 publications

(2 citation statements)

References 70 publications

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“…The results indicated that several members of NADH dehydrogenase (complex I) had been under pressure selection during evolution. Mitochondrial complex I is the first and largest respiratory complex required in the process of the process of oxidative phosphorylation to generate ATP, which is involved in the mitochondrial electron transport chain and is one of the major production sites of ROS (superoxide anion) ( Huang et al., 2016 ; Subrahmanian et al., 2016 ; Wang et al., 2022a ). Mutations in the members of complex I would change the metabolic capacity which may further affect adaptability of the organism ( Zhao et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of the complete mitochondrial genomes of three Coptis species (C. chinensis, C. deltoidea and C. omeiensis): the important medicinal plants in China

Zhong

et al. 2023

Front. Plant Sci.

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Coptis plants (Ranunculaceae) contain high levels of isoquinoline alkaloids and have a long history of medicinal use. Coptis species are of great value in pharmaceutical industries and scientific research. Mitochondria are considered as one of the central units for receiving stress signals and arranging immediate responses. Comprehensive characterizations of plant mitogenomes are imperative for revealing the relationship between mitochondria, elucidating biological functions of mitochondria and understanding the environmental adaptation mechanisms of plants. Here, the mitochondrial genomes of C. chinensis, C. deltoidea and C. omeiensis were assembled through the Nanopore and Illumina sequencing platform for the first time. The genome organization, gene number, RNA editing sites, repeat sequences, gene migration from chloroplast to mitochondria were compared. The mitogenomes of C. chinensis, C. deltoidea and C. omeiensis have six, two, two circular-mapping molecules with the total length of 1,425,403 bp, 1,520,338 bp and 1,152,812 bp, respectively. The complete mitogenomes harbors 68-86 predicted functional genes including 39-51 PCGs, 26-35 tRNAs and 2-5 rRNAs. C. deltoidea mitogenome host the most abundant repeat sequences, while C. chinensis mitogenome has the largest number of transferred fragments from its chloroplasts. The large repeat sequences and foreign sequences in the mitochondrial genomes of Coptis species were related to substantial rearrangements, changes in relative position of genes and multiple copy genes. Further comparative analysis illustrated that the PCGs under selected pressure in mitochondrial genomes of the three Coptis species mainly belong to the mitochondrial complex I (NADH dehydrogenase). Heat stress adversely affected the mitochondrial complex I and V, antioxidant enzyme system, ROS accumulation and ATP production of the three Coptis species. The activation of antioxidant enzymes, increase of T-AOC and maintenance of low ROS accumulation in C. chinensis under heat stress were suggested as the factors for its thermal acclimation and normal growth at lower altitudes. This study provides comprehensive information on the Coptis mitogenomes and is of great importance to elucidate the mitochondrial functions, understand the different thermal acclimation mechanisms of Coptis plants, and breed heat-tolerant varieties.

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Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of the complete mitochondrial genomes of three Coptis species (C. chinensis, C. deltoidea and C. omeiensis): the important medicinal plants in China

Zhong

et al. 2023

Front. Plant Sci.

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“…In this work, we identified and positionally cloned a novel maize ( Zea mays L.) crumpled kernel mutant ( crk2 ), named after crk1 (Wang et al ., 2022), which is caused by knockdown of ZmTFCB . We demonstrated that ZmTFCB participates in α‐tubulin folding, tubulin dimer formation, and regulation of MT dynamics through its interaction with three important partners, ZmCCT5, ZmTFCE, and ZmEB1.…”

Section: Introductionmentioning

confidence: 99%

Maize tubulin folding cofactor B is required for cell division and cell growth through modulating microtubule homeostasis

Zhou

et al. 2023

New Phytologist

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Summary Tubulin folding cofactors (TFCs) are required for tubulin folding, α/β tubulin heterodimer formation, and microtubule (MT) dynamics in yeast and mammals. However, the functions of their plant counterparts remain to be characterized. We identified a natural maize crumpled kernel mutant, crk2, which exhibits reductions in endosperm cell number and size, as well as embryo/seedling lethality. Map‐based cloning and functional complementation confirmed that ZmTFCB is causal for the mutation. ZmTFCB is targeted mainly to the cytosol. It facilitates α‐tubulin folding and heterodimer formation through sequential interactions with the cytosolic chaperonin‐containing TCP‐1 ε subunit ZmCCT5 and ZmTFCE, thus affecting the organization of both the spindle and phragmoplast MT array and the cortical MT polymerization and array formation, which consequently mediated cell division and cell growth. We detected a physical association between ZmTFCB and the maize MT plus‐end binding protein END‐BINDING1 (ZmEB1), indicating that ZmTFCB1 may modulate MT dynamics by sequestering ZmEB1. Our data demonstrate that ZmTFCB is required for cell division and cell growth through modulating MT homeostasis, an evolutionarily conserved machinery with some species‐specific divergence.

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Revisiting maize Brittle endosperm-2 reveals new insights in BETL development and starchy endosperm filling

et al. 2023

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An MCIA-like complex is required for mitochondrial complex I assembly and seed development in maize

Cited by 5 publications

References 70 publications

Comprehensive analysis of the complete mitochondrial genomes of three Coptis species (C. chinensis, C. deltoidea and C. omeiensis): the important medicinal plants in China

Comprehensive analysis of the complete mitochondrial genomes of three Coptis species (C. chinensis, C. deltoidea and C. omeiensis): the important medicinal plants in China

Maize tubulin folding cofactor B is required for cell division and cell growth through modulating microtubule homeostasis

Revisiting maize Brittle endosperm-2 reveals new insights in BETL development and starchy endosperm filling

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