An LDLa domain-containing C-type lectin is involved in the innate immunity of Eriocheir sinensis

Isolation and characterization of two novel C-type lectins from the oriental river prawn, Macrobrachium nipponense

“…As shown in Fig. 5, MnCTLDcp2 and MnCTLDcp3 was rapidly up-regulated to the highest level within 1 h and then decreased from 1 h, finally up-regulated at 36 he48 h which is similar to the expression pattern of EsCTLDcp from E. sinensis challenged with A. hydrophila [33]. Also, the expression of a C-type lectin TfCTL1 form Trachidermus fasciatus was stimulated by LPS challenge with a significant increase at 2 h post challenge, followed by three declines at 6 he24 h and rises at 48 h [38].…”

“…1A and 2), which was firstly identified from the CTLs of E. sinensis [32]. The N-terminal type A repeats in the LDL receptor involved in lipoprotein binding and the LDL receptor gene mutations may cause familial hypercholesterolemia [33]. The deduced MnCTLDcp2 and MnCTLDcp3 proteins contained a CTLD of 130 and 137 aa at the C-terminal, respectively.…”

“…An LDLa domain-containing C-type lectin EsCTLDcp [33] and three single-novel CRD containing C-type lectins TfCTL1, EsLecA and EsLecG [26,38] have been reported to agglutinate both Gram-positive and Gram-negative bacteria. In this research, MnCTLDcp2 and MnCTLDcp3 could also agglutinate two Grampositive bacteria (S. aureus and B. subtilis) and two Gramnegative bacteria (A. hydrophila and V. parahaemolyticus) (Fig.…”

Identification, characterization, and functional studies of a Pelle gene in the Chinese mitten crab, Eriocheir sinensis

“…Moreover, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was amplified for internal standardization by using the primers EsGAPDH-RT-F and EsGAPDH-RT-R. qRT-PCR was performed according to the manufacturer's instructions in the 2 Â SYBR Premix Ex Taq Kit (Takara, Japan) with a real-time thermal cycler (BioRad, Hercules, CA). The methods were performed as detailed in our previous study [35]. All the samples used for qRT-PCR analysis were prepared in triplicate.…”

“…In each experimental group (30 crabs), crabs received an injection of one of the following: about 50 ml of LPS (0.5 mg/ml), 50 ml of PGN (0.5 mg/ml), 50 ml of live S. aureus suspension (approximately 3 Â 10 7 cells), 50 ml of A. hydrophila (3 Â 10 5 cells), or 50 ml of V. parahaemolyticus (3 Â 10 7 cells). The preparation methods and pretreatment of the bacteria were performed according to the methods used in a previously published paper [35]. After treatment, the crabs were returned to the water tanks.…”