An LC/MS quantitative and microdialysis method for cyclovirobuxine D pharmacokinetics in rat plasma and brain: The pharmacokinetic comparison of three different drug delivery routes

In Vivo Pharmacokinetics of Puerarin via Different Drug Administration Routes Based on Middle Cerebral Artery Occlusion Model

“…Compared with the intravenous administration, the DTI was highly improved, as was reported in many studies based on healthy animals [15,16]. There were three pathways involving the transportation from nose to brain, including olfactory nerve pathway, trigeminal nerve pathway and vascular pathway.…”

“…By measuring the recovery of the probe and the concentration of the target compound in dialysates, the drug concentration of plasma and extracellular fluid of different tissues could be obtained. Nowadays, microdialysis is widely used in the pharmacokinetics study [9,10].…”

Mass spectrometric analysis of carisoprodol and meprobamate in rat brain microdialysates

“…Similarly to other assays we developed, [27,38] online desalting also could be carried out conveniently by diverting the effluent to waste and thereby avoiding laborious sample preparation such as liquid-liquid extraction also applied to in vivo microdialysates. [39] Meprobamate and diethyl acetamidomalonate eluted with retention time difference of about 0.1 min. Again, the resulting series of product-ion scans could be transformed into two separate SRM chromatograms thanks to the distinct masses of corresponding major fragments (m/z 158.1 for meprobamate and m/z 176.1 for diethyl acetamidomalonate).…”

“…Meprobamate and carisoprodol were separated well on a short octadecylsilica RP column under isocratic conditions with an acidified water/acetonitrile mobile phase. Similarly to other assays we developed, online desalting also could be carried out conveniently by diverting the effluent to waste and thereby avoiding laborious sample preparation such as liquid–liquid extraction also applied to in vivo microdialysates . Meprobamate and diethyl acetamidomalonate eluted with retention time difference of about 0.1 min.…”

New techniques of on-line biological sample processing and their application in the field of biopharmaceutical analysis

“…When compared with other hyphenated techniques, the LC—MS technique is generally applicable with MD for separation and analysis. In recent years, MD–LC–MS has been widely used in the pharmacy, biology, clinical medicine and other fields, especially in the field of biopharmaceutical analysis analyzing complex biological matrices such as whole blood 69 , 70 , plasma 71 , 72 , and urine 73 , 74 , etc.…”