An LC–MS/MS method for the simultaneous determination of chlorogenic acid, forsythiaside A and baicalin in rat plasma and its application to pharmacokinetic study of Shuang-huang-lian in rats

Ye, Jun-Xiao; Wang, Wei; Quan, Li-Hui; Liu, Chunyu; Chang, Qi; Liao, Yong-Hong

doi:10.1016/j.jpba.2010.01.035

Cited by 64 publications

(40 citation statements)

References 13 publications

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“…A comparative pharmacokinetic study of 124 in rats after administration of Shuang-huang-lian (SHL) solutions via i.v., peroral or intratracheal routes was reported [154]. The plasma concentration of 124 reached the peak at 45 min with C max of 35.0˘7.1 ng/mL after oral administration of 1000 mg/kg SHL solutions.…”

Section: Pharmacokinetics Of Forsythoside (124)mentioning

confidence: 96%

Phenylethanoid Glycosides: Research Advances in Their Phytochemistry, Pharmacological Activity and Pharmacokinetics

2016

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Phenylethanoid glycosides (PhGs) are widely distributed in traditional Chinese medicines as well as in other medicinal plants, and they were characterized by a phenethyl alcohol (C 6 -C 2 ) moiety attached to a β-glucopyranose/β-allopyranose via a glycosidic bond. The outstanding activity of PhGs in diverse diseases proves their importance in medicinal chemistry research. This review summarizes new findings on PhGs over the past 10 years, concerning the new structures, their bioactivities, including neuroprotective, anti-inflammatory, antioxidant, antibacterial and antivirus, cytotoxic, immunomodulatory, and enzyme inhibitory effects, and pharmacokinetic properties.

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Section: Pharmacokinetics Of Forsythoside (124)mentioning

confidence: 96%

Phenylethanoid Glycosides: Research Advances in Their Phytochemistry, Pharmacological Activity and Pharmacokinetics

2016

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“…In the last 10 years, large numbers of studies have focused mainly on the pharmacokinetics of baicalin, wogonoside, oroxylin A-7-glucuronide, baicalein, wogonin and oroxylin A in crude RS, single compenents or multiple compenents, five or six at most (Ye et al, 2010;Shi et al, 2011;Tong et al, 2012). However, to the best of our knowledge, there has been less attention on the pharmacokinetics of other flavonoids, including scutellarin, scutellarein and apigenin, from RS, pharmacokinetic studies of which only aimed directly at administration of a single substance or other herbal medicines.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of ten flavonoids of crude and wine‐processed Radix Scutellariae aqueous extracts in rat plasma by UPLC‐ESI‐MS/MS and its application to a comparative pharmacokinetic study

Cui

Qian

Huang

et al. 2014

Biomedical Chromatography

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Radix Scutellariae (RS) is a herbal medicine with various pharmacological activities to treat inflammation, respiratory and gastrointestinal infections, etc. In this study, a rapid, sensitive and selective UPLC-ESI-MS/MS method was developed for simultaneous determination of 10 flavonoids - scutellarin, scutellarein, chrysin, wogonin, baicalein, apigenin, wogonoside, oroxylin A-7-O-glucuronide, oroxylin A and baicalin - from RS aqueous extracts in rat plasma with propyl paraben as internal standard (IS). Chromatographic separation was achieved on a C18 column using gradient elution with the mobile phase consisting of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. The detection was performed in multiple reaction monitoring mode using electrospray ionization in negative mode. The validated method showed good linearity over a wide concentration range (r >0.9935). The intra- and interday assay variabilities were <9.5% and <12.4% for all analytes, respectively. The extraction recovery ranged from 71.2 to 89.7% for each analyte and IS. This method was successfully applied to pharmacokinetic comparision after oral administration of crude and wine-processed RS aqueous extracts. There were significant differences in some pharmacokinetic parameters of most analytes between crude and wine-processed RS. This suggested that wine-processing exerted effects absorption of most flavonoids.

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“…For example, Shuang-huang-lian (SHL) is a traditional Chinese medicine formula prepared from honeysuckle flower, radix Scutellariae and fructus Forsythiae, and has been traditionally used for the treatment of infectious diseases of the respiratory tract in China. An LC-MS/MS method was developed for the measurement of chlorogenic acid, forsythiaside A and baicalin in rat plasma using Q-Trap mass spectrometer (Applied Biosystems 3200 QTRAP) after SHL solutions were intravenously, perorally or intratracheally administered to rats [211]. The extracted analytes were separated on a reverse phase C12 column using acetonitrile and water containing 0.1% formic acid as mobile phase.…”

Section: Tcm Prescriptionsmentioning

confidence: 99%

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

Guo

Chen

et al. 2013

Journal of Pharmaceutical and Biomedical Analysis

161

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An LC–MS/MS method for the simultaneous determination of chlorogenic acid, forsythiaside A and baicalin in rat plasma and its application to pharmacokinetic study of Shuang-huang-lian in rats

Cited by 64 publications

References 13 publications

Phenylethanoid Glycosides: Research Advances in Their Phytochemistry, Pharmacological Activity and Pharmacokinetics

Phenylethanoid Glycosides: Research Advances in Their Phytochemistry, Pharmacological Activity and Pharmacokinetics

Simultaneous determination of ten flavonoids of crude and wine‐processed Radix Scutellariae aqueous extracts in rat plasma by UPLC‐ESI‐MS/MS and its application to a comparative pharmacokinetic study

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

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