An ISFET-based dipstick device for protein detection using the ion-step method

Enzyme Immunoassay Using a Reusable Extended-gate Field-Effect-Transistor Sensor with a Ferrocenylalkanethiol-modified Gold Electrode

“…To solve this key problem, the ion-step method for ISFET-based detection of lysozymes was introduced by Schasfoort et al 9 and was further investigated by other research groups. [10][11][12] The ion-step method is based on a dynamic measurement method in which an ISFET sensor detects the ionstep response of the release or uptake of protons by protein molecules bound onto the sensor. Additionally, indirect potentiometric detection using an ISFET sensor was combined with ELISA.…”

A novel enzyme immunoassay based on potentiometric measurement of molecular adsorption events by an extended-gate field-effect transistor sensor

“…To solve the above-mentioned problem, the ion-step method was first introduced for the ISFET-based detection of lysozymes by Schasfoort et al (1990b) and was further developed by other research groups (Van Kerhof et al, 1994;Eijkel et al, 1997;Besselink et al, 2003). The ion-step method is based on a dynamic measuring method, in which the ISFET sensor detects the ion-step response of the release or uptake of protons by protein molecules bound onto the ISFET sensor.…”

Modification of ISFETs with a monolayer of latex beads for specific detection of proteins

“…The so-called ion-step method represents a novel measurement concept for potentiometric detection and quantification of adsorbed biomolecules in which modified ISFETs are used. The ion-step method was first introduced by Schasfoort et al (1990a) and was further developed by Van Kerkhof et al (1994) and Eijkel et al (1997). The ion-step method is based on a dynamic measuring principle, whereas many other potentiometric methods are static and measure in a state of thermodynamic equilibrium.…”