An investigation on the effect of surface roughness of crystallization plate on protein crystallization

Hou, Hai; Wang, Bo; Hu, Shan-Yang; Wang, Meng-Ying; Feng, Jinyu; Xie, Peng-Peng; Yin, Da‐Chuan

doi:10.1016/j.jcrysgro.2016.10.007

Cited by 10 publications

(9 citation statements)

References 14 publications

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“…However, the crystal growth is restricted by the limited volume of the pit and results in smaller crystals. 37 For the sample Bamboo@MOF2 ( Fig. 4c and g ), a large number of MOF199 crystals with uniform sizes are distributed on the vessel surfaces of bamboo, and the pits can barely be distinguished.…”

Section: Resultsmentioning

confidence: 97%

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In situ deposition of MOF199 onto hierarchical structures of bamboo and wood and their antibacterial properties

Zhang

et al. 2019

RSC Adv.

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Section: Resultsmentioning

confidence: 97%

“…40 It is difficult for an over-rough surface to trap MOF precursors for nucleation because the surface contains large cavities which are similar to smooth surfaces for the molecules. 37 Therefore, fewer nucleation sites and sufficient space are conducive to the deposition of large MOF crystals. Fig.…”

Section: Surface Roughness Analysismentioning

confidence: 99%

In situ deposition of MOF199 onto hierarchical structures of bamboo and wood and their antibacterial properties

Zhang

et al. 2019

RSC Adv.

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“…HR4-506, Hampton Research, USA) was used to seal the crystallization plates. Protein crystallization plates named cross diffusion microbatch plates (CDM plates) developed from our lab were used for crystallization screening experiments . The dialysis bags (MD34-14-5, Solarbio, USA) were used to remove the salt and other small molecular species and concentrate the protein solutions.…”

Section: Methodsmentioning

confidence: 99%

“…More recently, the sharp decrease in the requirement of protein solution volume (from several microliters to several nanoliters) enabled large scale crystallization screening with the help of modern automation techniques. − Moreover, hundreds of commercialized crystallization screening kits are available for crystallization screening, , providing us with tremendous opportunities that were unimaginable in the past. In addition to the screening methods, the implementation of many new methods to increase the nucleation, such as the utilization of numerous different nucleants, − microseeding, desiccation, , cross-diffusion microbatch (CDM), − and so on, largely increased the opportunities to determine the crystallization conditions.…”

Section: Introductionmentioning

confidence: 99%

Direct Crystallization of Proteins from Impure Sources

Liu

Hou

et al. 2020

Crystal Growth & Design

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In recent years, with the rapidly increasing demand for pure protein products in various fields (biomedicines, biochemical reagents, food industries, etc.), the need for low-cost, high-quality protein purification technology has become urgent. Under this background, the traditional purification technology, protein crystallization, comes back to people’s attention. Protein crystallization has the ability to obtain high-quality protein products at a low cost. Nevertheless, protein crystallization itself is challenging; for a long time, the industrial purification of proteins often has used chromatography-based approaches. In the field of structural biology, the strong demand for protein crystals has led to the full development of protein crystallization technology. To date, these technologies may have the potential to provide solutions to achieve crystallization of proteins at the industrial scale. In this paper, we report our effort to screen the crystallization conditions of four sample proteins (lysozyme, hemoglobin, superoxide dismutase, and homoserine oxygen-acetyltransferase) from different impure sources (natural and recombined ones). It was confirmed that crystallization screening technology allows protein crystals to be obtained directly from impure protein sources, showing that, from the impure sources, the target protein can be purified directly by crystallization without prior purification using chromatographic processes. This work demonstrated that the new technologies developed in the field of protein crystallization methodologies can be well applied in solving problems in traditional purification technology.

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“…The surface roughness (up to 3 nm) and wettability properties were correlated with the amount of TCDB deposited [19] and with the protein crystallization outcome. Topography effects were also investigated by modifying the surface of conventional protein crystallization plates with various types of wet oxidation treatments [20] in order to generate different degrees of roughness (up to 32 nm).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques

Polino

Portugal

Tiggelaar³

et al. 2020

Crystal Growth & Design

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In this work, the influence of surface topography on protein crystallization over Nafion® is investigated. Two types of Nafion® based membranes were modified by soft lithographic techniques in order to create different topographies at the micro and nano scale and subsequently tested. From the analysis of the induction time, nucleation and crystal growth rate of Trypsin from Bovine Pancreas, all the patterned Nafion® based membranes show an enhanced nucleation and crystal growth. To provide additional insight to the experimental observations, the wettability properties of the prepared samples and the ratio of the Gibbs free energy of heterogeneous nucleation to homogeneous nucleation were evaluated. The crystallization outcome results from the combined effect of both, the structural and chemical properties of the nucleant Nafion® surface.

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An investigation on the effect of surface roughness of crystallization plate on protein crystallization

Cited by 10 publications

References 14 publications

In situ deposition of MOF199 onto hierarchical structures of bamboo and wood and their antibacterial properties

In situ deposition of MOF199 onto hierarchical structures of bamboo and wood and their antibacterial properties

Direct Crystallization of Proteins from Impure Sources

Enhanced Protein Crystallization on Nafion Membranes Modified by Low-Cost Surface Patterning Techniques

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