An investigation of thiol/disulfide homeostasis and ischemia-modified albumin levels to assess the oxidative stress in dogs with canine distemper

Abstract

This study aimed to investigate the diagnostic value of thiol/disulfide homeostasis (TDH) and ischemia-modified albumin (IMA) levels in evaluating oxidative stress in dogs with canine distemper. The study materials consisted of 24 cross-breed dogs: 18 dogs with canine distemper (CD group) and 6 healthy dogs as the control. Blood samples for analysis of antioxidant-oxidant parameters were taken from both groups. In addition to TDH parameters (native thiol [NT], total thiol [TT], and disulfide [Ds] levels and Ds/NT, Ds/TT, and NT/TT ratios), IMA and albumin (ALB) levels were also determined in the blood serum of the groups. The IMA levels of the CD group were significantly higher than the control group (P<0.05). Antioxidant parameters (NT, TT) and oxidant parameters (Ds, Ds/NT, and Ds/TT) both exhibited a significant positive correlation among themselves (P<0.01). The areas under the ROC curve of IMA, Ds/NT, and Ds/TT were 0.78, 0.62, and 0.62 respectively. The high levels of IMA, Ds, Ds/NT, and Ds/TT and low levels of NT, TT, NT/TT and ALB in dogs with canine distemper can be used to assess the oxidative stress caused by the disease. Overall, the diagnostic performances of IMA, Ds/NT, and Ds/TT for detection of increased oxidative stress were similar, but IMA was superior.

Dynamic thiol-disulphide homeostasis and ischemia modified albumin levels in neonatal calf diarrhea

“…Although many parameters, indicate oxidative stress in gastrointestinal system diseases, have been evaluated in the field of veterinary medicine in the current literature, there is no study investigating IMA levels. In a study on dogs with Canine Distemper (7), IMA, disulfide, % disulfide/native thiol ratio and % disulfide/total thiol ratio levels were higher, while native thiol, total thiol, % native thiol/total thiol ratio and albumin levels were significantly lower than the control group, and this was interpreted as canine distemper increased oxidative stress in dogs. The results obtained in our study are parallel to the studies conducted in the field of human and veterinary medicine, both in terms of TDH parameters and IMA levels.…”

Investigation of Aopp, Total Sulphydryl Group (T-sh), Seroplasmin Levels in Sheep Wi̇th Zoonotic Liver Parasitosis

“…(Yazıcı et al, 2002).Çetinkaya et al, (2020) reported that total thiol parameter was signi cantly lower in rat models of Alzheimer's disease compared to the control group. Similarly, Çamkerten et al,(2019) reported that total sulfhydryl group was importantly lower in pigs with sarcoptyx mange compared to healthy pigs Değirmençay et al, (2021). revealed that thiol levels were low in dogs with canine distemper.…”

“…Değirmençay et al, 2021). Thiol groups, which try to prevent the damaging effect of free radicals, can not maintain plasma and tissue levels during these effects.…”

Evaluation of Apoptosis and Autophagy Activities in the Brain of Dogs Naturally Infected with Canine Distemper Virus Based on Changes in Apoptotic and Autophagic Markers

“…The condition has been known to affect dogs and other carnivores and is one of the most infectious viral diseases with the highest fatality rate in dogs besides rabies [2]. Canine distemper virus (CDV) causes a multisystemic infection including the gastrointestinal, respiratory and nervous systems [3,4]. As a result of the viral spread to the central nervous system (CNS), multifocal demyelination occurs [3].…”