An Investigation of The Effect of Extracellular Vesicles Isolated from Mouse Embryonic Fibroblasts on Wound Healing in an Experimental Diabetic Mouse Model

Abstract

This study aimed to investigate the effects of Extracellular Vesicles (EV) secreted from mouse embryonic fi broblasts EV on wound healing of full-thickness skin defect in a diabetic mouse model. The study included both in vitro and in vivo experimental studies 82 mice. In the in vitro stage of the experimental study, hysterectomy was performed on two mice between the 13-14th days of pregnancy and then EV was isolated by cell culturing.VEGF, IL-6, and TNF-α biomarkers were examined in tissue homogenate. Moreover, tissue taken from wound area was also subjected to histopathologic scoring. EV augmented th e effect of VEGF. Therefore promoted angiogenesis increases the transport of cells, essential oxygen and nutrients in the wound area. Extracellular vesicles isolated from mouse embryonic fi broblasts have been found to accelerate wound healing in diabetes.The fi ndings obtained from this experimental study indicate that EV isolated from mouse embryonic fi broblasts accelerate the wound healing process in experimentally induced-diabetes in mice.

Modulation of immune cum inflammatory pathway by earthworm granulation tissue extract in wound healing of diabetic rabbit model

“…In present study, we successfully demonstrated wound healing properties of EGTE in rabbit model via gross findings, oxidative stress, connective tissue markers, pro-inflammatory markers and expression of growth markers which are considered as most important parameters for medicinal preparations having wound healing potential [ 7 , 9 , 11 ]. In recent times researchers have proposed that biomedical preparations obtained from biologics having abundant concentration of growth factors can accelerate wound healing in diabetic patients [ 13 ].…”

“…Individuals diagnosed with diabetes face an increased risk of developing diabetic ulcers, a perplexing and highly distressing complication that further complicates their diabetes management [ 9 , 10 ]. The healthcare costs associated with treating these diabetic ulcers impose a substantial economic burden on patients, and these ulcers also exhibit a high recurrence rate, affecting approximately 50 % of diabetic individuals [ 11 , 12 ]. Researchers have identified several factors contributing to impaired diabetic wound healing, including reduced blood vessel formation, impaired skin cell regeneration, hindered fibroblast migration, and elevated levels of Tumor Necrosis Factor alpha (TNF-α) [ 13 , 14 ].…”

Extracellular vesicles derived from fibroblasts induced with or without high glucose exert opposite effects on wound healing and angiogenesis

“…Under normal physiological condition, EVs derived from dermal fibroblasts promote diabetic cutaneous wound healing through the Akt/β-catenin pathway ( 10 ). Another report showed that EVs isolated from mouse embryonic fibroblasts augmented the effect of VEGF, promoting angiogenesis in the wound area ( 11 ). Gangadaran et al ( 12 ) have already confirmed the angiogenic cargoes in human fibroblast-derived EVs.…”