An investigation of the effect of thiamine pyrophosphate on cisplatin-induced oxidative stress and DNA damage in rat brain tissue compared with thiamine

Abstract

<jats:p> This study investigated the effects of thiamine pyrophosphate (TPP) at dosages of 10 and 20 mg/kg on oxidative stress induced in rat brain tissue with cisplatin and compared this with thiamine. Cisplatin neurotoxicity represents one of the main restrictions on the drug being given in effective doses. Oxidative stress is considered responsible for cisplatin toxicity. Our results showed that cisplatin increased the levels of oxidant parameters such as lipid peroxidation (thio barbituric acid reactive substance (TBARS)) and myeloperoxidase (MPO) in brain tissue and suppressed the effects of antioxidants such as total glutathione (GSH) and superoxide dismutase (SOD). TPP, especially at a dosage of 20 mg/kg, significantly reduced TBARS and MPO levels that increase with cisplatin administration compared with the thiamine group, while TPP significantly increases GSH and SOD levels. In addition, the level of 8-Gua (guanine), a product of DNA damage, was 1.7 ± 0.12 8-hydroxyl guanine (8-OH Gua)/105 Gua in brain tissue in the control group receiving cisplatin, compared with 0.97 ± 0.03 8-OH Gua/105 Gua in the thiamine pyrophosphate (20 mg/kg) group and 1.55 ± 0.11 8-OH Gua/105 Gua in the thiamine (20 mg/kg) group. These results show that thiamine pyrophosphate significantly prevents oxidative damage induced by cisplatin in brain tissue, while the protective effect of thiamine is insignificant. </jats:p>

Can thıamıne pyrophosphate prevent desflurane ınduced hepatotoxıcıty ın rats?

“…The anesthetic gas vaporizer was calibrated before beginning of the study. In performing the experiment, TDG group was intraperitoneally (ip) injected 20 mg/kg TPP 10,11 . The HG and DCG groups were administered distilled water through the same route.…”

“…Another study reported that TPP significantly decreased MPO value which was increased with cisplatine in the cerebral tissue, in a dose dependent manner 10 . We found that the values of these antioxidant parameters increased in TPP administered group.…”

Chemotherapy-induced neurotoxicity in pediatric solid non-CNS tumor patients: An update on current state of research and recommended future directions

“…(1) Methotrexate inhibits metabolism of folate acid, which is necessary for the synthesis of the nucleoside thymidine in DNA synthesis (2) [75,79,[83][84][85]. We will now discuss how these markers are related to specific neurotoxic pathways.…”

Содержание Витаминов И Микроэлементов У Подростков С Диффузным Нетоксическим Зобом С Учетом Потребления Продуктов Питания

“…Ви-тамин В 2 в составе фермента йодтирозиндейодиназы высвобождает йодид из моно-и дийодтирозина [17]. Доказана роль витамина В 1 в синтезе тиреоидных гормонов и в антиоксидантной защите ЩЖ [19]. Проведенные нами ранее исследования свиде-тельствуют о наличии у детей и подростков с ДНЗ микроэлементного и витаминного дисбаланса, ха-рактер которого зависит от возраста, пола подростка и морфофункционального состояния ЩЖ [2,8,9].…”