An investigation of the carbon nanotube – Lipid interface and its impact upon pulmonary surfactant lipid function

Melbourne, Jodie; Clancy, Adam J.; Seiffert, Joanna; Skepper, Jeremy N.; Tetley, Teresa D.; Shaffer, Milo S. P.; Porter, Alexandra E.

doi:10.1016/j.biomaterials.2015.03.023

Cited by 15 publications

(11 citation statements)

References 48 publications

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“…Loss of nanomaterial to the subphase was also indicated by NPs effects on both DPPC and PL films since penetration of NPs into the monolayers would shift the compression isotherms to higher molecular areas. Thus, the shifting of the DPPC isotherm to lower molecular areas would indicate that NPs could somehow sequester DPPC molecules once translocating to the subphase [41,42]. On the other hand, the finding that isotherms of PL films following deposition of NPs were identical to the control suggests that NPs do not remain associated but translocate to the subphase.…”

Section: Discussionmentioning

confidence: 99%

Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant

et al. 2021

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Polyhydroxyalkanoates (PHA) are polyesters produced intracellularly by many bacterial species as energy storage materials, which are used in biomedical applications, including drug delivery systems, due to their biocompatibility and biodegradability. In this study, we evaluated the potential application of this nanomaterial as a basis of inhaled drug delivery systems. To that end, we assessed the possible interaction between PHA nanoparticles (NPs) and pulmonary surfactant using dynamic light scattering, Langmuir balances, and epifluorescence microscopy. Our results demonstrate that NPs deposited onto preformed monolayers of DPPC or DPPC/POPG bind these surfactant lipids. This interaction facilitated the translocation of the nanomaterial towards the aqueous subphase, with the subsequent loss of lipid from the interface. NPs that remained at the interface associated with liquid expanded (LE)/tilted condensed (TC) phase boundaries, decreasing the size of condensed domains and promoting the intermixing of TC and LE phases at submicroscopic scale. This provided the stability necessary for attaining high surface pressures upon compression, countering the destabilization induced by lipid loss. These effects were observed only for high NP loads, suggesting a limit for the use of these NPs in pulmonary drug delivery.

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Section: Discussionmentioning

confidence: 99%

Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant

et al. 2021

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“…For carbon nanotubes, numerous in vivo experimental studies showed that carbon nanotubes can penetrate deep into the lung and reach the subpleural region (Ryman-Rasmussen et al, 2009; Li et al, 2007; Mercer, et al, 2010, 2011). Due to the importance of pulmonary surfactant as the first biological barrier to contact with airbone nanomaterials, extensive in vivo and in vitro experiments have also been done to study interaction between carbon nanotubes and PS monolayer at the air-water interface (Kapralov et al, 2012; Lee et al, 2013; Melbourne et al, 2015; Valle et al, 2015; Kadoya et al, 2016). Experimental studies of MWCNT-pulmonary surfactant interaction and its effect on PS functionality using a Langmuir-Blodgett trough show that the length and concentration of carbon nanotubes significantly influence the compression resistance of the film (Melbourne et al, 2015).…”

Section: Testing Strategies For High Aspect Ratio Nanomaterials (Hmentioning

confidence: 99%

“…Due to the importance of pulmonary surfactant as the first biological barrier to contact with airbone nanomaterials, extensive in vivo and in vitro experiments have also been done to study interaction between carbon nanotubes and PS monolayer at the air-water interface (Kapralov et al, 2012; Lee et al, 2013; Melbourne et al, 2015; Valle et al, 2015; Kadoya et al, 2016). Experimental studies of MWCNT-pulmonary surfactant interaction and its effect on PS functionality using a Langmuir-Blodgett trough show that the length and concentration of carbon nanotubes significantly influence the compression resistance of the film (Melbourne et al, 2015). In an in vitro study of pulmonary surfactants on a constrained drop surfactometer (CDS), compression-expansion loop shows a very high hysteresis when exposed carbon nanotubes, which could pose a danger to normal stable respiration (Valle et al, 2015).…”

Section: Testing Strategies For High Aspect Ratio Nanomaterials (Hmentioning

confidence: 99%

“…In vitro experiments show that the size and concentration of carbon nanotubes significantly change the mechanical response of the surfactant monolayer (Valle et al, 2015; Melbourne et al, 2015). Recent coarse-grained molecular dynamics simulations suggest that ultrashort SWCNTs (less than 5.5 nm) can insert into the surfactant monolayer via self-rotation (Yue et al, 2017), where the interaction morphology is determined by the length and diameter of SWCNTs and membrane tension of the surfactant (Xu et al, 2017).…”

Section: Testing Strategies For High Aspect Ratio Nanomaterials (Hmentioning

confidence: 99%

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The asbestos-carbon nanotube analogy: An update

Kane

Hurt

Gao

2018

Toxicology and Applied Pharmacology

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Nanotechnology is an emerging industry based on commercialization of materials with one or more dimensions of 100 nm or less. Engineered nanomaterials are currently incorporated into thin films, porous materials, liquid suspensions, or filler/matrix nanocomposites with future applications predicted in energy and catalysis, microelectronics, environmental sensing and remediation, and nanomedicine. Carbon nanotubes are one-dimensional fibrous nanomaterials that physically resemble asbestos fibers. Toxicologic studies in rodents demonstrated that some types of carbon nanotubes can induce mesothelioma, and the World Health Organization evaluated long, rigid multiwall carbon nanotubes as possibly carcinogenic for humans in 2014. This review summarizes key physicochemical similarities and differences between asbestos fibers and carbon nanotubes. The "fiber pathogenicity paradigm" has been extended to include carbon nanotubes as well as other high-aspect-ratio fibrous nanomaterials including metallic nanowires. This paradigm identifies width, length, and biopersistence of high-aspect-ratio fibrous nanomaterials as critical determinants of lung disease, including mesothelioma, following inhalation. Based on recent theoretical modeling studies, a fourth factor, mechanical bending stiffness, will be considered as predictive of potential carcinogenicity. Novel three-dimensional lung tissue platforms provide an opportunity for in vitro screening of a wide range of high aspect ratio fibrous nanomaterials for potential lung toxicity prior to commercialization.

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“…The PS film adsorbs onto the air–water interface of the alveoli. It plays a vital role in reducing the surface tension of the lungs to maintain the proper mechanics and to avoid alveolar collapse. , Dipalmitoylphosphatidylcholine (DPPC) is the most abundant single component in natural PS. Hence, the DPPC monolayer self-assembled at the air–water surface has been widely used as a PS model to understand the biophysical role of PS films. − The DPPC monolayer experiences phase transitions between the liquid-expanded (LE) and the liquid-condensed (LC) phases during compression and expansion processes. ,− Research shows that PAMAM dendrimers can disrupt lipid bilayers. − However, it is still unclear whether inhaled PAMAM dendrimers have adverse impacts on the function of PS films.…”

Section: Introductionmentioning

confidence: 99%

Poly(amidoamine) Dendrimer as a Respiratory Nanocarrier: Insights from Experiments and Molecular Dynamics Simulations

et al. 2019

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Pulmonary drug delivery is superior to the systemic administration in treating lung diseases. An optimal respiratory nanocarrier should be able to efficiently and safely cross the pulmonary surfactant film, which serves as the first biological barrier for respiratory delivery and plays paramount roles in maintaining the proper mechanics of breathing. In this work, we focused on the interactions between poly-(amidoamine) (PAMAM) dendrimers and a model pulmonary surfactant. With combined Langmuir monolayer experiments and coarse-grained molecular dynamics simulations, we studied the effect of environmental temperature, size, and surface property of PAMAM dendrimers (G3-OH, G3-NH 2 , G5-OH, and G5-NH 2 ) on the dipalmitoylphosphatidylcholine (DPPC) monolayer. Our simulations indicated that the environmental temperature could significantly affect the influence of PAMAM dendrimers on the DPPC monolayer. Therefore, results obtained at room temperature cannot be directly applied to elucidate interactions at body temperature. Simulations at body temperature found that all tested PAMAM dendrimers can easily penetrate the lipid monolayer during the monolayer expansion process (mimicking "inhalation"), and the cationic PAMAM dendrimers (−NH 2 ) show promising penetration ability during the monolayer compression process (mimicking "expiration"). Larger PAMAM dendrimers (G5) adsorbed onto the lipid monolayer tend to induce structural collapse and inhibit normal phase transitions of the lipid monolayer. These adverse effects could be mitigated in the subsequent expansion−compression cycle. These findings suggest that the PAMAM dendrimer may be used as a potential respiratory drug nanocarrier.

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An investigation of the carbon nanotube – Lipid interface and its impact upon pulmonary surfactant lipid function

Cited by 15 publications

References 48 publications

Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant

Polyhydroxyalkanoate Nanoparticles for Pulmonary Drug Delivery: Interaction with Lung Surfactant

The asbestos-carbon nanotube analogy: An update

Poly(amidoamine) Dendrimer as a Respiratory Nanocarrier: Insights from Experiments and Molecular Dynamics Simulations

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