An Investigation into the Cytotoxic Effects of 13-Acetoxysarcocrassolide from the Soft Coral Sarcophyton crassocaule on Bladder Cancer Cells

Abstract

Active compounds from natural products have been widely studied. The anti-tumor effects of 13-acetoxysarcocrassolide isolated from Formosan soft coral Sarcophyton crassocaule on bladder cancer cells were examined in this study. An MTT assay showed that 13-acetoxysarcocrassolide was cytotoxic to bladder female transitional cancer (BFTC) cells. We determined that the BFTC cells underwent cell death through apoptosis by flow cytometry. Due to the highly-migratory nature of the BFTC cells, the ability of 13-acetoxysarcocrassolide to stop their migration was assessed by a wound healing assay. To determine which proteins were affected in the BFTC cells upon treatment, a comparative proteomic analysis was performed. By LC-MS/MS analysis, we identified that 19 proteins were up-regulated and eight were down-regulated. Seven of the proteins were confirmed by western blotting analysis. This study reveals clues to the potential mechanism of the cytotoxic effects of 13-acetoxysarcocrassolide on BFTC cells. Moreover, it suggests that PPT1 and hnRNP F could be new biomarkers for bladder cancer. The results of this study are also helpful for the diagnosis, progression monitoring and therapeutic strategies of transitional cell tumors.

Proteomic analysis indicates the importance of TPM3 in esophageal squamous cell carcinoma invasion and metastasis

“…Cytoskeleton rearrangement altered the activity of colon cancer cells, which was associated with MYL12B (17). Su et al (18) observed that the overexpression of hnRNP F influenced the development of colon tumors and interfered with normal apoptosis and energy metabolism of bladder cells, which could result in bladder cancer. DES, TPM3, TPM2 and vimentin are important in maintaining the stability of the cytoskeleton, and changes in vimentin expression were associated with liver cancer metastasis and recurrence (19).…”

Improvement and enhancement of antibladder carcinoma cell effects of heteronemin by the nanosized hyaluronan aggregation

“…2,9,24 The concentrations of protein were determined by bicinchoninic acid assay according to the manufacturer's instructions. In all, 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to run and separate the protein samples.…”

“…It has been found that several natural compounds from soft corals have cytotoxic and apoptosis-inducing effects against melanoma cells, hepatocellular carcinoma cells, and bladder cancer cells in our previous studies. 2,9,10 Heteronemin (HET) is a known sesterterpenoid extracted from the sponge Hippospongia sp. This natural marine compound was found to inhibit the enzyme protein farnesyltransferase and reduce the viability of leukemia cells, colon cancer cells, and breast cancer cells.…”

“…Bladder cancer accounts for the sixth most common cancer among men (19th in women) in an estimated 14.1 million cancer cases around the world in 2012. The importance of bladder cancer and its therapies should [1][2][3] Urothelial bladder cancer has significant morbidity, mortality, and impact on public health. 3 Approximately 75% of diagnosed urothelial bladder cancer is present as superficial and nonmuscle-invasive UC as the rest is classified into aggressive muscle-invasive UC.…”

Pertumbuhan Karang Lunak Sarcophyton sp. yang Dibudidayakan di Teluk Awur, Jepara

“…memiliki kemampuan menghalangi pertumbuhan sedikitnya satu dari tujuh isolat pembentuk bio-filum. S. crassocaule diketahui memiliki potensi sitotoksik terhadap sel kanker kandung kemih (Su et al, 2011). Sarcophyton juga dinyatakan mengandung diterpenoid laut yang dapat dimanfaatkan sebagai agen anti-inflamasi (Gonzalez et al, 2015).…”