Proteomic analysis indicates the importance of TPM3 in esophageal squamous cell carcinoma invasion and metastasis

Yu, Shaobin; Gao, Qi; Lin, Wen-Wei; Kang, Myounggu

doi:10.3892/mmr.2017.6145

Cited by 18 publications

(19 citation statements)

References 25 publications

(26 reference statements)

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“…Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with multiple cancers, including BC. Multiple studies have proved that TPM3 could promote the invasion of tumor cells [17,[25][26][27][28]. Consistent with previous studies, our results showed the expression level of TPM3 mRNA in the platelets was significantly increased in the platelets from patients with metastasis compared with the patients without metastasis.…”

Section: Discussionsupporting

confidence: 92%

Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis

Yao

et al. 2019

FEBS Open Bio

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Platelets are implicated in the pathophysiology of breast and other cancers through their role in exchanging biomolecules with tumor cells in the tumor microenvironment. Such exchange results in tumor‐educated platelets with altered RNA expression profiles. Multiple lines of evidence indicate that platelet RNA profiles may be suitable as diagnostic biomarkers for cancer‐related biological processes. In this study, we characterized the gene expression signatures of platelets in breast cancer (BC) by high‐throughput sequencing and quantitative real‐time RT‐PCR. Our results indicate that the expression of TPM3 (tropomyosin 3) mRNA is significantly elevated in platelets from patients with BC compared with age‐matched healthy control subjects. Furthermore, up‐regulation of TPM3 mRNA in platelets was found to be significantly correlated with metastasis in patients with BC. Finally, we report that platelet TPM3 mRNA is delivered into BC cells through microvesicles and leads to enhanced migrative phenotype of BC cells. In summary, our findings suggest that the transfer of platelet TPM3 mRNA into cancer cells via microvesicles promotes cancer cell migration, and thus platelet‐derived TPM3 mRNA may be a suitable biomarker for early diagnosis of metastatic BC.

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Section: Discussionsupporting

confidence: 92%

Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis

Yao

et al. 2019

FEBS Open Bio

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“…29,30 To investigate the effect of pristimerin on migration and invasion of ESCC cells, wound healing and transwell assays were conducted in EC9706 and EC109 cells. Invasion and metastasis are key factors related to poor prognosis following esophagectomy in patients with ESCC.…”

Section: Pristimerin Inhibits Migration and Invasion Of Escc Cellsmentioning

confidence: 99%

“…Invasion and metastasis are key factors related to poor prognosis following esophagectomy in patients with ESCC. 29,30 To investigate the effect of pristimerin on migration and invasion of ESCC cells, wound healing and transwell assays were conducted in EC9706 and EC109 cells. The expression of PARP, caspase 3, and active-caspase 3 was detected by western blotting.…”

Section: Pristimerin Inhibits Migration and Invasion Of Escc Cellsmentioning

confidence: 99%

Pristimerin targeting NF‐κB pathway inhibits proliferation, migration, and invasion in esophageal squamous cell carcinoma cells

Tan

Zhou

et al. 2018

Cell Biochemistry & Function

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Although several approaches including surgery, chemotherapy, and radiotherapy had been applied in the treatment of ESCC, more effective targeted chemotherapies are required to increase the survival rates of patients. This study suggested that inhibiting NF-κB signalling pathway could be an effective approach for the treatment of ESCC. Pristimerin, a potent NF-κB inhibitor, exerted potent anti-ESCC effects both in vitro and in vivo, which may be a promising therapeutic agent for ESCC.

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“…TPM3 belongs to the tropomyosin super family, which can be viewed as a universal regulator of the actin cytoskeleton for cellular functions such as cell proliferation ( 20 ). The expression levels of TPM3 are higher in stage III esophageal squamous cell carcinoma tissue compared with stage I ( 21 ), and glucose glycated TPM3 suppresses colon cancer cell Caco-2 proliferation ( 22 ). In immortalized mouse embryonic fibroblasts, TPM3 is partly responsible for cell proliferation via regulating the interaction between pERK and Imp7, which is overridden by Ras transformation ( 23 ).…”

Section: Discussionmentioning

confidence: 99%

Nicotine promotes the development of oral leukoplakia via regulating peroxiredoxin 1 and its binding proteins

Tang

et al. 2021

Braz J Med Biol Res

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Tobacco can induce reactive oxygen species (ROS) production extensively in cells, which is a major risk factor for oral leukoplakia (OLK) development. Peroxiredoxin 1 (Prx1) is a key antioxidant protein, upregulated in a variety of malignant tumors. We previously found that nicotine, the main ingredient of tobacco, promotes oral carcinogenesis via regulating Prx1. The aim of the present study was to screen and identify the Prx1 interacting proteins and investigate the mechanisms of nicotine on the development of OLK. Through liquid chromatography-tandem mass spectrometry combined with bioinformatics analysis, the candidate Prx1 interacting proteins of cofilin-1 (CFL1), tropomyosin alpha-3 chain (TPM3), and serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A) were screened in human dysplastic oral keratinocyte cells treated with nicotine. CFL1, TPM3, and PPP2R1A were highly expressed in human OLK tissues. The expression of CFL1 increased and the expression of PPP2R1A decreased in OLK of smokers compared to that in OLK of non-smokers. Nicotine upregulated CFL1 and downregulated PPP2R1A in 4-nitro-quinoline-1-oxide (4NQO)-induced OLK tissues in mice in part dependent on Prx1. Furthermore, the in-situ interaction of CFL1, TPM3, and PPP2R1A with Prx1 were validated in human OLK tissues. Our results suggested that tobacco might promote the development of OLK via regulating Prx1 and its interacting proteins CFL1 and PPP2R1A.

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Proteomic analysis indicates the importance of TPM3 in esophageal squamous cell carcinoma invasion and metastasis

Cited by 18 publications

References 25 publications

Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis

Delivery of platelet TPM3 mRNA into breast cancer cells via microvesicles enhances metastasis

Pristimerin targeting NF‐κB pathway inhibits proliferation, migration, and invasion in esophageal squamous cell carcinoma cells

Nicotine promotes the development of oral leukoplakia via regulating peroxiredoxin 1 and its binding proteins

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