An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes

Muenthaisong, Anucha; Nambooppha, Boondarika; Rittipornlertrak, Amarin; Tankaew, Pallop; Varinrak, Thanya; Muangthai, Korkiat; Atthikanyaphak, Kheemchompu; Sawada, Takuo; Sthitmatee, Nattawooti

doi:10.1155/2020/3548973

Cited by 15 publications

(14 citation statements)

References 28 publications

(55 reference statements)

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“…Immunogenic response on experimental exposure to LPS of P. multocida in rabbits has also been reported from Pakistan (Ashraf et al, 2014). Recently a recombinant OMP based intranasal vaccine has been successfully experimented in swamp buffaloes against HS (Muenthaisong et al, 2020).…”

Section: Hematology and Serum Biochemistrymentioning

confidence: 99%

Effect of Exposure to Pasteurella multocida B:2 and its Immunogens on Clinical, Hematological and Serum Biochemical Parameters in Neeli Ravi Buffaloes

Zaman¹,

Roohi²,

Irfan³

2021

SJA

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B uffalo is considered the main dairy animal in Pakistan. Almost 76% of total buffaloes are found in Punjab and the remaining 24% of the buffaloes are found in other provinces (Anonymous, 2019-20). The total number of buffaloes in Pakistan is 41.2 Million, while 73% of the national milk production Abstract | Buffaloes and other bovines suffer from a fatal disease, "Hemorrhagic septicemia" (HS), which is produced by a gram-negative bacterium "Pasteurella multocida." Existing literature has established bacteria's pathogenesis, but still, the literature about the role of bacteria and its immunogens on the demonstration of clinical signs, hematology and serum biochemistry in Nili Ravi buffaloes under subtropics is lacking. This study was designed to fulfill this gap by analyzing the effects of exposure to the bacterial culture of Pasturella multocida (P. multocida) type B:2 and its immunogens, i.e., lipopolysaccharide (LPS) and outer membrane protein (OMP) on Nili Ravi buffaloes. Prepubertal Nili Ravi female buffaloes (N=18) of 10-12 months age in good health condition were divided into five treatment groups (n=3 each) and a control group (n=3). Treatment groups' animals were exposed to P. multocida bacterial culture and its immunogens, i.e., LPS (oral and intravenous) and OMP (oral and subcutaneous). Animals were analyzed for the development of clinical signs, hematology and serum biochemistry. All treatment groups exhibited clinical signs (ranging from mild to acute) like increased temperature, variation in rumen motility, mucous membrane, salivation, edema, dullness, depression, nasal discharge, respiratory distress and recumbence or death. A significant change in complete blood count (CBC) including packed cell volume (PCV), hemoglobin (Hgb) concentration, red blood cells (RBCs) count, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) platelets count, mean platelet volume (MPV), white blood cells (WBCs) count, monocytes count, lymphocytes count and granulocytes count. Serum biochemistry profiles (total protein, Albumin and creatinine) of treatment group animals were also changed significantly. Cumulatively it was revealed that P. multocida B: 2 and its Immunogens (LPS and OMP) affect the hematology and serum biochemistry profiles of Neeli-Ravi buffaloes. These findings prove that OMP and LPS from P. multocida B: 2 may be considered for vaccine development.

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Section: Hematology and Serum Biochemistrymentioning

confidence: 99%

Effect of Exposure to Pasteurella multocida B:2 and its Immunogens on Clinical, Hematological and Serum Biochemical Parameters in Neeli Ravi Buffaloes

Zaman¹,

Roohi²,

Irfan³

2021

SJA

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“…The OmpH based vaccine has been used for protecting swamp buffaloes from hemorrhagic septicemia in South Asian countries. [49]. OmpH has other functions as well such as in Pseudomonas aeruginosa, it provides stability to the outer membrane through interaction with lipopolysaccharide [50].…”

Section: Ompa and Omph Family Proteinmentioning

confidence: 99%

"Review on Fusobacterium necrophorum Putative Candidates for Vaccine Development Strategies: Outer Membrane Proteins and Outer Membrane Vesicles"

Narayanan¹

2022

BJSTR

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Fusobacterium necrophorum is a Gram-negative, strictly anaerobic bacterium associated with necrotic infections in animals and humans. The bacterium is an opportunistic and primary pathogen that causes liver abscess, footrot, and laryngeal infections in cattle. Liver abscess in cattle is reported at 20.7% annually, leading to liver condemnation and economic burden to the feedlot industry. Antibiotics are the mainstay for treatment; however, the reports of antibiotic resistance and demand for antibiotic-free, natural and organic beef have demanded alternative therapies and preventatives. Hence, developing an effective vaccine is essential to control infections and economic loss to the cattle industry. Currently, there is no licensed vaccine to prevent liver abscesses in cattle. A number of virulence factors for F. necrophorum have been explored in the past for vaccine development. Each one has some advantages and disadvantages concerning immunogenicity and protective effect. The review summarizes vaccine candidates explored in the past, mainly focusing on F. necrophorum. The review also connects some concepts related to virulence factors found in F. necrophorum and how it could be a promising vaccine candidate based on the studies done in other Gram-negatives. Mini ReviewFusobacterium necrophorum, a Gram-negative anaerobic bacillus, is an opportunistic pathogen isolated from oral cavities, gastrointestinal and genitourinary tracts of humans and animals [1,2]. The bacterium is associated with Lemierre's syndrome affecting young and healthy individuals and necrotic infections in hepatic, abdominal, and respiratory organs in animals [3,4]. The bacteria are primary causative agents of liver abscess, foot rot, and calf diphtheria, sometimes in mixed infections with other bacteria such as Trueperella pyogenes and Porphyromonas species [5,6]. F. necrophorum has been classified into four biotypes: A, B, AB, and C [7]. Biotypes A and B are of veterinary importance and associated with cattle liver abscesses. Biotype A, subspecies necrophorum, and Biotype B, subspecies funduliforme, vary in cell morphology, colony characteristics, virulence capacity, virulence factors, 16s rRNA sequences, and DNA gyrase B subunit [7,8]. The subspecies necrophorum is more virulent and frequently isolated than the subspecies funduliforme, and is the primary causative agent in liver abscesses [9]. Annually, the rate of incidence of liver abscess in feedlot cattle is 10-20% [10].Generally, the incidence is higher in cattle fed with high grainbased diets where the progression occurs from chronic acidosis and rumenitis to liver abscess [11]. The National Beef Quality

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“…Goat challenge models have been described [353,354]. Experimental challenge and vaccine assessment has also been established in dairy cattle and buffalo [367][368][369][370][371].…”

Section: Hemorrhagic Septicemiamentioning

confidence: 99%

Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences

Clemmons

Alfson

Dutton

2021

Animals

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Animals provide food and other critical resources to most of the global population. As such, diseases of animals can cause dire consequences, especially disease with high rates of morbidity or mortality. Transboundary animal diseases (TADs) are highly contagious or transmissible, epidemic diseases, with the potential to spread rapidly across the globe and the potential to cause substantial socioeconomic and public health consequences. Transboundary animal diseases can threaten the global food supply, reduce the availability of non-food animal products, or cause the loss of human productivity or life. Further, TADs result in socioeconomic consequences from costs of control or preventative measures, and from trade restrictions. A greater understanding of the transmission, spread, and pathogenesis of these diseases is required. Further work is also needed to improve the efficacy and cost of both diagnostics and vaccines. This review aims to give a broad overview of 17 TADs, providing researchers and veterinarians with a current, succinct resource of salient details regarding these significant diseases. For each disease, we provide a synopsis of the disease and its status, species and geographic areas affected, a summary of in vitro or in vivo research models, and when available, information regarding prevention or treatment.

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An Intranasal Vaccination with a Recombinant Outer Membrane Protein H against Haemorrhagic Septicemia in Swamp Buffaloes

Cited by 15 publications

References 28 publications

Effect of Exposure to Pasteurella multocida B:2 and its Immunogens on Clinical, Hematological and Serum Biochemical Parameters in Neeli Ravi Buffaloes

Effect of Exposure to Pasteurella multocida B:2 and its Immunogens on Clinical, Hematological and Serum Biochemical Parameters in Neeli Ravi Buffaloes

"Review on Fusobacterium necrophorum Putative Candidates for Vaccine Development Strategies: Outer Membrane Proteins and Outer Membrane Vesicles"

Transboundary Animal Diseases, an Overview of 17 Diseases with Potential for Global Spread and Serious Consequences

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