An Interpretation Model For The UV-VIS Spectra Of Microorganisms

Alupoaei, Catalina E.; García‐Rubio, Luis H.

doi:10.1080/00986440590473326

Cited by 34 publications

(49 citation statements)

References 27 publications

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“…The results of Alupoaei and Garcia-Rubio (2005) combined with previous research efforts using the same interpretation model (Alupoaei, 2001;Callahan et al, 2003; showed that there is a potential for multiwavelength spectroscopy to be used as a rapid analytical technique that can be applied in order to detect, identify, and quantify microorganisms. The modeled spectrum is based on light scattering and absorption theories, and the approximation of the frequency-dependant optical properties of the basic constituents of living organisms.…”

Section: Introductionmentioning

confidence: 63%

“…Recently, Alupoaei and Garcia-Rubio (2005) formulated an interpretation model for the multiwavelength spectra of microorganisms. The results of Alupoaei and Garcia-Rubio (2005) combined with previous research efforts using the same interpretation model (Alupoaei, 2001;Callahan et al, 2003; showed that there is a potential for multiwavelength spectroscopy to be used as a rapid analytical technique that can be applied in order to detect, identify, and quantify microorganisms.…”

Section: Introductionmentioning

confidence: 99%

“…The potential exists to reformulate the model proposed by Alupoaei and Garcia-Rubio (2005) to interpret spectra of K. brevis through multiwavelength spectroscopy in order to reflect the cellular complexity ( Fig. 1) specifically inherent to K. brevis.…”

Section: Introductionmentioning

confidence: 99%

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Progress in developing a new detection method for the harmful algal bloom species, Karenia brevis, through multiwavelength spectroscopy

et al. 2009

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Section: Introductionmentioning

confidence: 63%

Section: Introductionmentioning

confidence: 99%

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Progress in developing a new detection method for the harmful algal bloom species, Karenia brevis, through multiwavelength spectroscopy

et al. 2009

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“…coli is a typical gram-negative, nonspore, rod-shaped bacterium, whose main chemical components are protein and nucleic acid (approximately 80 percent of the cell's dry weight), its minor constituents, carbohydrate (8.4%), lipid (9.1%), and coenzyme (2.5%) [36,37]. Chemical composition of microorganisms, such as protein, nucleic acid, and dipicolinic acid, plays a significant role in the absorption of ultraviolet light [38]. Figure 5 shows a comparison of the absorption coefficient of nucleic acid, chromophoric amino acids, and dipicolinic acid weighted by their typical concentration versus wavelength.…”

Section: Spectral Analysis: Scattering Componentmentioning

confidence: 99%

Analytic Method on Characteristic Parameters of Bacteria in Water by Multiwavelength Transmission Spectroscopy

Yu-xia

Zhao

Gan

et al. 2017

Journal of Spectroscopy

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An analytic method together with the Mie scattering theory and Beer-Lambert law is proposed for the characteristic parameter determination of bacterial cells (Escherichia coli 10389) from multiwavelength transmission spectroscopy measurements. We calculate the structural parameters of E. coli cells, and compared with the microscopy, the relative error of cell volume is 7.90%, the cell number is compared with those obtained by plate counting, the relative error is l.02%, and the nucleic content and protein content of single E. coli cells are consistent with the data reported elsewhere. The proposed method can obtain characteristic parameters of bacteria as an excellent candidate for the rapid detection and identification of bacteria in the water.

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“…Ultraviolet and visible (UV-Vis) spectroscopy, which measures the amount of light that is attenuated by a solution or a suspension of particles, is a quantitative, reliable, and rapid analytical tool that can be immediately applied as a biosensor for the detection, identification, and enumeration of cells (An and Friedman, 1997;Alupoaei et al, 2004;Alupoaei and García-Rubio, 2005). UV-Vis spectroscopy of cells has been used to estimate the number of cells in a solution and their chemical composition, including nucleic acid and protein concentrations (Leštan et al, 1993;Wang et al, 1995;Piereira et al, 2000;Alupoaei, 2001;Salard et al, 2001;Alupoaei and García-Rubio, 2005). Based on the current uses of UV-Vis spectroscopy, it is feasible that a detection scheme could be developed to distinguish live and dead cells based on UV-Vis spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

Park

Yoon

Byeon³

et al. 2012

Aerosol Air Qual. Res.

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We evaluated a method for the assessment of bacterial viability that is based on ultraviolet and visible (UV-Vis) spectroscopy analysis. The quantities of intracellular materials inside a cell vary depending on change of bacterial viability by disruption of the membrane integrity. Therefore, normalized optical density in the range of 200-290 nm was analyzed to determine if it varied in samples containing different proportions of live bacteria. Our results indicate that samples containing higher proportions of live bacteria such as Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis had higher optical densities. In addition, the optical density at 230 nm divided by the optical density at 670 nm was found to have a strong linear correlation with bacterial viability (the R 2 values of E. coli, B. subtilis, and S. epidermidis are 0.9964, 0.9118, and 0.9861, respectively). Our proposed rapid assessment method takes less than three minutes and only requires optical measurements at 230 and 670 nm; therefore, it is simpler and faster than colony counting, fluorochromasia, or the dyeexclusion test. Moreover, our method was applied to bioaerosols, which are currently important issues in public health, microbiology, aerosol science, and other fields. In our study, the bacteria (E. coli) were dispersed into the air using a Collisontype atomizer, and were sampled in sterilized deionized water using an impinger with a pump. According to our method, the viability of E. coli was approximately 55.2%, which was similar to 52.5 ± 4.7% determined from the LIVE/DEAD BacLight bacterial viability assay.

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An Interpretation Model For The UV-VIS Spectra Of Microorganisms

Cited by 34 publications

References 27 publications

Progress in developing a new detection method for the harmful algal bloom species, Karenia brevis, through multiwavelength spectroscopy

Progress in developing a new detection method for the harmful algal bloom species, Karenia brevis, through multiwavelength spectroscopy

Analytic Method on Characteristic Parameters of Bacteria in Water by Multiwavelength Transmission Spectroscopy

Development of Rapid Assessment Method to Determine Bacterial Viability Based on Ultraviolet and Visible (UV-Vis) Spectroscopy Analysis Including Application to Bioaerosols

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