An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection

Ida, Jeunice; Kuzuya, Akinori; Choong, Yee Siew; Lim, Theam Soon

doi:10.1039/d0ra05439a

Cited by 15 publications

(10 citation statements)

References 50 publications

(48 reference statements)

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“…It can be hypothesized that the lower limit of detection of this study is 0.00008 nM as any concentration less than 0.00008 nM is not detectable. The detection limit obtained was then compared with some of the recently published data as shown in Table 2 , which clearly shows that the Au/DSU/NH 2 rGO–PAMAM/IgM sensor film-based SPR sensor has the lowest detection limit so far [ 62 , 63 , 73 , 81 , 82 ]. Herein, the inclusion of DSU as a self-assembly monolayer and NH 2 rGO–PAMAM composite as a sensing layer has provided strong support for IgM immobilization for selective detection of dengue virus.…”

Section: Resultsmentioning

confidence: 86%

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

et al. 2021

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This paper proposes a novel idea to enhance the sensitivity and selectivity of surface plasmon resonance (SPR) optical sensor for detection of dengue virus type-2 envelope proteins (DENV-2 E-proteins) using polyamidoamine (PAMAM) dendrimer biopolymer-based nanocomposite thin film. For this purpose, two ranges of DENV-2 E-protein concentrations, i.e., 0.000008–0.0001 nM and 0.00008–0.005 nM were evaluated, and the lowest detectable concentration was achieved at 0.00008 nM. The incorporation of PAMAM dendrimer-based nanocomposite thin film with an SPR sensor exhibited a significant increase in sensitivity and binding affinity to a lower range DENV-2 E-protein concentrations. Moreover, the proposed sensor displayed good selectivity towards DENV-2 E-proteins and have an average recovery of 80–120%. The findings of this study demonstrated that PAMAM dendrimer-based nanocomposite thin film combined with SPR sensor is a promising diagnostic tool for sensitive and selective detection of DENV-2 E-proteins.

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Section: Resultsmentioning

confidence: 86%

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

et al. 2021

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“…By easily substituting the hybridization segment of the probe to the sequence complementary of the target, this platform can be adapted for various nucleic acid detection assays of other viruses. Although our approach is more sensitive than the previously reported PCR-free, simple operational split G-quadruplex/hemin DNAzyme detection assays for dengue virus [ 36 ], its ability to detect actual virus mRNA might still be limited and further validation of the assay in various norovirus-infected samples is also required. In further studies, the diG4/hemin DNAzyme might be combined with non-enzymatic DNA amplification techniques such as catalyzed hairpin assembly (CHA) so as to further improve the sensitivity of measurement, and thus, to provide a simple, cost-effective and high-sensitivity biosensing system to promote the early screening of communicable diseases such as acute gastroenteritis caused by norovirus in underdeveloped areas.…”

Section: Discussionmentioning

confidence: 99%

Design of a High-Sensitivity Dimeric G-Quadruplex/Hemin DNAzyme Biosensor for Norovirus Detection

Zhang

et al. 2021

Molecules

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G-quadruplexes can bind with hemin to form peroxidase-like DNAzymes that are widely used in the design of biosensors. However, the catalytic activity of G-quadruplex/hemin DNAzyme is relatively low compared with natural peroxidase, which hampers its sensitivity and, thus, its application in the detection of nucleic acids. In this study, we developed a high-sensitivity biosensor targeting norovirus nucleic acids through rationally introducing a dimeric G-quadruplex structure into the DNAzyme. In this strategy, two separate molecular beacons each having a G-quadruplex-forming sequence embedded in the stem structure are brought together through hybridization with a target DNA strand, and thus forms a three-way junction architecture and allows a dimeric G-quadruplex to form, which, upon binding with hemin, has a synergistic enhancement of catalytic activities. This provides a high-sensitivity colorimetric readout by the catalyzing H2O2-mediated oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline -6-sulfonic acid) diammonium salt (ABTS). Up to 10 nM of target DNA can be detected through colorimetric observation with the naked eye using our strategy. Hence, our approach provides a non-amplifying, non-labeling, simple-operating, cost-effective colorimetric biosensing method for target nucleic acids, such as norovirus-conserved sequence detection, and highlights the further implication of higher-order multimerized G-quadruplex structures in the design of high-sensitivity biosensors.

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“…G-quadruplex-hemin DNAzyme has a similar function to HRP, which catalyzes the oxidation of colorless 2,2-azinobis(3-ethylbenzothiozoline)-6-sulfonicacid (ABTS2 − ) to the green ABTS radical by H 2 O 2 . This principle has been applied for the detection of HBV gene [ 126 ], DNA related to HIV [ 127 ], and dengue virus (DENV) [ 128 ]. Yin et al [ 129 ] constructed a visual biosensor with polystyrene (PS) electrospun nanofibrous membrane as a basement to enhance the DNAzyme catalysis efficiency.…”

Section: Applications Of Fnas For Infectious Diseasesmentioning

confidence: 99%

Selection and applications of functional nucleic acids for infectious disease detection and prevention

et al. 2021

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Infectious diseases caused by pathogenic microorganisms such as viruses and bacteria pose a great threat to human health. Although a significant progress has been obtained in the diagnosis and prevention of infectious diseases, it still remains challenging to develop rapid and cost-effective detection approaches and overcome the side effects of therapeutic agents and pathogen resistance. Functional nucleic acids (FNAs), especially the most widely used aptamers and DNAzymes, hold the advantages of high stability and flexible design, which make them ideal molecular recognition tools for bacteria and viruses, as well as potential therapeutic drugs for infectious diseases. This review summarizes important advances in the selection and detection of bacterial-and virus-associated FNAs, along with their potential prevention ability of infectious disease in recent years. Finally, the challenges and future development directions are concluded.

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An intermolecular-split G-quadruplex DNAzyme sensor for dengue virus detection

Abstract: Application of split G-quadruplex as DNAzyme reporter system for DNA sensing.

Cited by 15 publications

References 50 publications

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

An Optical Sensor for Dengue Envelope Proteins Using Polyamidoamine Dendrimer Biopolymer-Based Nanocomposite Thin Film: Enhanced Sensitivity, Selectivity, and Recovery Studies

Design of a High-Sensitivity Dimeric G-Quadruplex/Hemin DNAzyme Biosensor for Norovirus Detection

Selection and applications of functional nucleic acids for infectious disease detection and prevention

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