An integrity assay for a meningococcal type B conjugate vaccine

Turula, Vincent E.; Kim, John; Michon, Francis; Pankratz, James; Zhang, Kun; Yoo, Chul Gyu

doi:10.1016/j.ab.2004.02.001

Cited by 11 publications

(3 citation statements)

References 23 publications

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“…Methyl glycosides are relatively stable products compared with monosaccharides released under aqueous conditions and can be subjected to harsher hydrolyses with minimal degradation (Turula et al, 2004; WhitWeld et al, 1991). Pneumococcal polysaccharides were subjected to methanolysis as the sole hydrolysis step, followed by derivatization and chromatography on a GC–MSD.…”

Section: Discussionmentioning

confidence: 99%

Development and evaluation of a novel vaccine against prevalent invasive multi-drug resistant strains ofStreptococcus pneumoniae

Bahy

Hamouda

Shahat

et al. 2016

PeerJ

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Streptococcus pneumoniae is a pathogen that causes serious invasive infections, such as septicemia, meningitis and pneumonia in addition to mild upper respiratory tract infections. Protection from pneumococcal diseases is thought to be mediated mainly by serotype-specific antibodies to capsular antigens. Pneumococcal conjugate vaccine consists of sugars (polysaccharides) from the capsule of the bacterium S. pneumoniae that are conjugated to a carrier protein. Three pneumococcal conjugated vaccines, each directed against a group of serotypes, are registered in Egypt; however, local vaccine production is required to cover the most prevalent serotypes. In this work, capsular polysaccharide from the most current and prevalent serotypes in Egypt were extracted, purified and conjugated to bovine serum albumin (BSA). The polysaccharide protein conjugate was purified through ultrafiltration technique and molecular size distribution was compared to an available vaccine. The immunogenicity of the prepared vaccine was examined via two methods: First, by measuring the levels of the elicited antibodies in the sera of the vaccinated mice; second, by challenging the vaccinated groups of mice with approximately 107 CFU of each specific serotype and determining the degree of protection the developled vaccine offers. Our results show that the developed conjugated capsular polysaccharide vaccine is highly immunogenic and protective in mice. This finding illustrates the importance of tracking the most recent and predominant peneumococcal serotypes to generate effective vaccines, instead of using expensive imported vaccines with large number of serotypes which might not be even present in the community.

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Section: Discussionmentioning

confidence: 99%

Development and evaluation of a novel vaccine against prevalent invasive multi-drug resistant strains ofStreptococcus pneumoniae

Bahy

Hamouda

Shahat

et al. 2016

PeerJ

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“…Various methods for the analysis of sialyl oligosaccharides have been reported. High‐pH anion‐exchange chromatography with pulse amperometric detection, capillary electrophoresis, LC with ultraviolet absorbance detection, and LC with fluorescence detection have been used. Although the sensitivity of these methods is fairly good for the analysis of sialyl oligosaccharides, the identification of each sialyl oligosaccharide from its retention time may not be always possible because other saccharides may be co‐eluted with the analytes.…”

Section: Structures Of Sialyl Oligosaccharides Used In This Studymentioning

confidence: 99%

Liquid chromatography/tandem mass spectrometry with fluorous derivatization method for selective analysis of sialyl oligosaccharides

Sakaguchi¹,

Hayama

Yoshida

et al. 2014

Rapid Comm Mass Spectrometry

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“…In 2004 a group from Baxter Healthcare in Beltsville, Maryland, published an HPAE-PAD assay to judge the integrity of a vaccine targeting serotype B. 31 The polysaccharide is an α2,8-linked linear homopolymer of Neu5Ac. They discovered that this polysaccharide was not amenable to acid hydrolysis when conjugated to the carrier protein.…”

Section: Neisseria Meningitidis Vaccinesmentioning

confidence: 99%

Vaccine Quality Ensured by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

Rohrer

2020

SLAS Technology

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An integrity assay for a meningococcal type B conjugate vaccine

Cited by 11 publications

References 23 publications

Development and evaluation of a novel vaccine against prevalent invasive multi-drug resistant strains ofStreptococcus pneumoniae

Development and evaluation of a novel vaccine against prevalent invasive multi-drug resistant strains ofStreptococcus pneumoniae

Liquid chromatography/tandem mass spectrometry with fluorous derivatization method for selective analysis of sialyl oligosaccharides

Vaccine Quality Ensured by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection

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