An Integrated Optical Biosensor (IOBS)

Seifert, M.; Tiefenthaler, K.; Heuberger, K.; Lukosz, W.; Mosbach, Klaus

doi:10.1080/00032718608066252

Cited by 19 publications

(4 citation statements)

References 8 publications

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“…High chemical specificity/selectivity of the sensitized surface, coupled with high sensitivity of planar optical waveguide sensor in most cases, gives the possibility to develop immunosensors with lower detection limit than traditionally used immunoassays (Adanyi et al, 2006;Cummins et al, 2006). If a stable biologically active layer can be formed on the waveguide surface, which is regenerable, the sensor can be calibrated with a single sensitized chip and, a large number of samples can be analyzed (Seifert et al, 1986;Ramsden, 1993, Ramsden and Vergeres, 1999;Clerc and Lukoz, 1997). Similar type of work for glucose oxidase enzyme using refractive index detection was demonstrated using metal clad leaky waveguides (MCLW) sensor (Zourob and Goddard, 2005), by binding of protein-A to antiprotein-A, immobilized in agarose.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of sucrose biosensor based on single mode planar optical waveguide using co-immobilized plant invertase and GOD

Bagal

Vijayan

Aiyer

et al. 2007

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Fabrication of sucrose biosensor based on single mode planar optical waveguide using co-immobilized plant invertase and GOD

Bagal

Vijayan

Aiyer

et al. 2007

Biosensors and Bioelectronics

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“…Optical waveguide lightmode spectroscopy (OWLS) was developed in the mid-1980s and is a label-free technique that has proven useful in studying adsorption, desorption, adhesion, and biospecific binding processes (Kurrat et al, 1998;Lukosz, 1995;Lukosz and Tiefenthaler, 1988;Ramsden, 1992Ramsden, , 1993aRuiz et al, 1999;Seifert et al, 1986;Vörös et al, 2002). Linearly polarized light (e.g., from an He-Ne laser) is coupled into a waveguide layer by a diffraction grating at two well-defined incident angles corresponding to the transverse electric and transverse magnetic polarization modes.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical optical waveguide lightmode spectroscopy (EC‐OWLS): A pilot study using evanescent‐field optical sensing under voltage control to monitor polycationic polymer adsorption onto indium tin oxide (ITO)‐coated waveguide chips

Bearinger

Vörös

Hubbell

et al. 2003

Biotech & Bioengineering

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A new technique has been developed that combines evanescent-field optical sensing with electrochemical control of surface adsorption processes. This new technique, termed "electrochemical optical waveguide lightmode spectroscopy" (EC-OWLS), proved efficient in monitoring molecular surface adsorption and layer thickness changes of an adsorbed polymer layer examined in situ as a function of potential applied to a waveguide in a pilot study. For optical sensing, a layer of indium tin oxide (ITO) served as both a high-refractive-index waveguide and a conductive electrode. In addition, an electrochemical flow-through fluid cell was provided, which incorporated working, reference, and counter electrodes, and was compatible with the constraints of optical sensing. Poly(L-lysine)-grafted-poly(ethylene glycol) (PLL-g-PEG) served as a model, polycation adsorbate. Adsorption of PLL-g-PEG from aqueous buffer solution increased from 125 to 475 ng/cm(2 )along a sigmoidal path as a function of increasing potential between 0 and 1.5 V versus the Ag reference electrode. Upon buffer rinse, adsorption was partially reversible when a potential of >/=0.93 V was maintained on the ITO waveguide. However, reducing the applied potential back to 0 V before rinsing resulted in irreversible polymer adsorption. PLL-g-PEG modified with biotin demonstrated similar adsorption characteristics, but subsequent streptavidin binding was independent of biotin concentration. Applying positive potentials resulted in increased adsorbed mass, presumably due to polymer chain extension and reorganization in the molecular adlayer.

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“…Soon the interest was directed to biosensor application. The input grating coupler was used to observe an enzymatic reaction [239] or to measure protein adsorption (human immunoglobulin G, h-IgG) [240,241]. Finally the implementation of integrated input grating couplers as direct immunosensors is discussed with optical requirements and biochemical experiments [242].…”

Section: Waveguides With External Periodic Structurementioning

confidence: 99%

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

Gauglitz

2020

Anal Bioanal Chem

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Direct optical detection has proven to be a highly interesting tool in biomolecular interaction analysis to be used in drug discovery, ligand/receptor interactions, environmental analysis, clinical diagnostics, screening of large data volumes in immunology, cancer therapy, or personalized medicine. In this review, the fundamental optical principles and applications are reviewed. Devices are based on concepts such as refractometry, evanescent field, waveguides modes, reflectometry, resonance and/or interference. They are realized in ring resonators; prism couplers; surface plasmon resonance; resonant mirror; Bragg grating; grating couplers; photonic crystals, Mach-Zehnder, Young, Hartman interferometers; backscattering; ellipsometry; or reflectance interferometry. The physical theories of various optical principles have already been reviewed in detail elsewhere and are therefore only cited. This review provides an overall survey on the application of these methods in direct optical biosensing. The "historical" development of the main principles is given to understand the various, and sometimes only slightly modified variations published as "new" methods or the use of a new acronym and commercialization by different companies. Improvement of optics is only one way to increase the quality of biosensors. Additional essential aspects are the surface modification of transducers, immobilization strategies, selection of recognition elements, the influence of non-specific interaction, selectivity, and sensitivity. Furthermore, papers use for reporting minimal amounts of detectable analyte terms such as value of mass, moles, grams, or mol/L which are difficult to compare. Both these essential aspects (i.e., biochemistry and the presentation of LOD values) can be discussed only in brief (but references are provided) in order to prevent the paper from becoming too long. The review will concentrate on a comparison of the optical methods, their application, and the resulting bioanalytical quality.

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An Integrated Optical Biosensor (IOBS)

Cited by 19 publications

References 8 publications

Fabrication of sucrose biosensor based on single mode planar optical waveguide using co-immobilized plant invertase and GOD

Fabrication of sucrose biosensor based on single mode planar optical waveguide using co-immobilized plant invertase and GOD

Electrochemical optical waveguide lightmode spectroscopy (EC‐OWLS): A pilot study using evanescent‐field optical sensing under voltage control to monitor polycationic polymer adsorption onto indium tin oxide (ITO)‐coated waveguide chips

Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects

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