An integrated network pharmacology and metabolomics approach to reveal the immunomodulatory mechanism of <i>Brassica rapa</i> L. (Tibetan Turnip) in fatigue mice

Zhu, Hongkang; Yang, Yan; Li, Fulin; Wang, Xiaoyan; He, Qian

doi:10.1039/d2fo02308c

Cited by 5 publications

(4 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The taurine and hypotaurine metabolism is an energy metabolic homeostasis-related signaling pathway. According to the observations of Zhu et al, Brassica rapa L. extract could strengthen energy metabolism in mice and help against fatigue through up-regulating taurine and hypotaurine metabolism pathways [21]. In this study, increasing the OPL to OPO ratio in HMFS also significantly up-regulated the taurine and hypotaurine metabolism in a dose-dependent manner, which provides further evidence for the significantly higher expressions of two key thermogenic proteins (peroxisome proliferators-activated receptor γ coactivator lalpha and uncoupling protein 1) in perirenal adipose tissue of HMFS-fed rats, especially for the rats fed with HMFS3 (OPL to OPO ratio of 1.4) [22].…”

Section: Metabolomics Analysismentioning

confidence: 99%

Effects of sn-2 Palmitic Triacylglycerols and the Ratio of OPL to OPO in Human Milk Fat Substitute on Metabolic Regulation in Sprague-Dawley Rats

Zhu,

Fang,

Zhang

et al. 2024

Nutrients

View full text Add to dashboard Cite

In this study, the influence of total sn-2 palmitic triacylglycerols (TAGs) and ratio of 1-oleoyl-2-palmitoyl-3-linoleoylglycerol (OPL) to 1,3-dioleoyl-2-palmitoylglycerol (OPO) in human milk fat substitute (HMFS) on the metabolic changes were investigated in Sprague–Dawley rats. Metabolomics and lipidomics profiling analysis indicated that increasing the total sn-2 palmitic TAGs and OPL to OPO ratio in HMFS could significantly influence glycine, serine and threonine metabolism, glycerophospholipid metabolism, glycerolipid metabolism, sphingolipid metabolism, bile acid biosynthesis, and taurine and hypotaurine metabolism pathways in rats after 4 weeks of feeding, which were mainly related to lipid, bile acid and energy metabolism. Meanwhile, the up-regulation of taurine, L-tryptophan, and L-cysteine, and down-regulations of lysoPC (18:0) and hypoxanthine would contribute to the reduction in inflammatory response and oxidative stress, and improvement of immunity function in rats. In addition, analysis of targeted biochemical factors also revealed that HMFS-fed rats had significantly increased levels of anti-inflammatory factor (IL-4), immunoglobulin A (IgA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-px), and decreased levels of pro-inflammatory factors (IL-6 and TNF-α) and malondialdehyde (MDA), compared with those of the control fat-fed rats. Collectively, these observations present new in vivo nutritional evidence for the metabolic regulatory effects of the TAG structure and composition of human milk fat substitutes on the host.

show abstract

Section: Metabolomics Analysismentioning

confidence: 99%

Effects of sn-2 Palmitic Triacylglycerols and the Ratio of OPL to OPO in Human Milk Fat Substitute on Metabolic Regulation in Sprague-Dawley Rats

Zhu,

Fang,

Zhang

et al. 2024

Nutrients

View full text Add to dashboard Cite

show abstract

“…It is considered a valid aid to evaluate the pharmacological activities of phytochemical constituents. 18 Our previous work found that black quinoa polyphenols (BQP) showed a strong positive correlation with the inhibitory ability on α-glucosidase and exhibited potential hypoglycemic activity. Therefore, this study was aimed at assessing whether BQP can work against the state of IR and investigating the related constituents and exploring the mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…Network pharmacology is a method to reveal the latent interactions among the drug, ingredients, genes, and diseases based on the systems biology theory. It is considered a valid aid to evaluate the pharmacological activities of phytochemical constituents . Our previous work found that black quinoa polyphenols (BQP) showed a strong positive correlation with the inhibitory ability on α-glucosidase and exhibited potential hypoglycemic activity.…”

Section: Introductionmentioning

confidence: 99%

Phenolic Constituents from Black Quinoa Alleviate Insulin Resistance in HepG2 Cells via Regulating IRS1/PI3K/Akt/GLUTs Signaling Pathways

Cao,

Wang,

Jiang

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

“…Our recent research has identified numerous constituents in the aqueous extract of Brassica rapa L. (AEB), including organic acids, esters, thioglycosides, glycosides, flavonoids, polyphenols, etc. [8]. We have also assessed its potential in reducing acute exercise-induced fatigue and its muscle-protective properties.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Extract of Brassica rapa L.’s Impact on Modulating Exercise-Induced Fatigue via Gut–Muscle Axis

Wang,

Zhu,

Cheng

et al. 2023

Nutrients

Self Cite

View full text Add to dashboard Cite

Exercise-induced fatigue is a common physiological response to prolonged physical activity, often associated with changes in gut microbiota and metabolic responses. This study investigates the potential role of Brassica rapa L. in modulating these responses. Using an animal model subjected to chronic exercise-induced stress, we explored the effects of Brassica rapa L. on fatigue-related biomarkers, energy metabolism genes, inflammatory responses, intestinal integrity, and gut microbiota composition. Our findings revealed that Brassica rapa L. exhibits significant antioxidant activity and effectively modulates physiological responses to fatigue. It influences gene expression related to the tricarboxylic acid (TCA) cycle in muscle tissue through the AMPK/PGC-1α/TFAM signaling pathway. Furthermore, Brassica rapa L. has been found to alleviate inflammation by inhibiting lipopolysaccharide (LPS) infection and suppressing the activation of the NF-κB pathway. It also maintains intestinal integrity and controls Gram-negative bacterial growth. A correlation analysis identified several pathogenic bacteria linked with inflammation and energy metabolism, as well as beneficial probiotic bacteria associated with improved energy metabolism and reduced inflammation. These findings underscore Brassica rapa L.’s potential for managing prolonged exercise-induced fatigue, paving the way for future therapeutic applications. The results highlight its impact on gut microbiota modulation and its role in nutrition science and sports medicine.

show abstract

An integrated network pharmacology and metabolomics approach to reveal the immunomodulatory mechanism of Brassica rapa L. (Tibetan Turnip) in fatigue mice

Abstract: Brassica rapa L. has been widely used as an edible, feeding and medical plant on the Qinghai-Tibet Plateau, for several pharmacological effects of reinforcing deficiency or weakness, anti-inflammation, and relieving...

Cited by 5 publications

References 42 publications

Effects of sn-2 Palmitic Triacylglycerols and the Ratio of OPL to OPO in Human Milk Fat Substitute on Metabolic Regulation in Sprague-Dawley Rats

Effects of sn-2 Palmitic Triacylglycerols and the Ratio of OPL to OPO in Human Milk Fat Substitute on Metabolic Regulation in Sprague-Dawley Rats

Phenolic Constituents from Black Quinoa Alleviate Insulin Resistance in HepG2 Cells via Regulating IRS1/PI3K/Akt/GLUTs Signaling Pathways

Aqueous Extract of Brassica rapa L.’s Impact on Modulating Exercise-Induced Fatigue via Gut–Muscle Axis

Contact Info

Product

Resources

About