An integrated lab-on-a-disc for automated cell-based allergen screening bioassays

Altered basophil function in patients with chronic kidney disease on hemodialysis

“…The drug screening to suppress the fMLP-mediated degranulation in basophils was recently  -Fluid viscosity Q -Vol. flow rate w -Channel width h -Channel height 2 6 wh Q    examined using an integrated centrifugal microfluidic platform (Kwok et al 2016, Chen et al 2012. Furthermore, a chip-cytometry technique has been used for quantification of bead-based purified basophils from bronchoalveolar lavage (BAL) and blood in asthmatic patients (Dijkstra et al 2014).…”

Label-free impedance detection of cancer cells from whole blood on an integrated centrifugal microfluidic platform

“…Nwankire et al, 2013). LoaD platforms have also been developed for cell handling, bioassays (Boettcher et al, 2006;Burger and Ducrée, 2012;Chen et al, 2012;Lee et al, 2014a) and have also been interfaced with electrical systems for real-time flow monitoring (Abi-Samra et al, 2013). For cell detection, electrochemical schemes are beneficial due to their ability to provide highly sensitive, rapid real-time quantitative information, based on simple instrumentation.…”

Allergy Testing and Drug Screening on an ITO-Coated Lab-on-a-Disc

“…Flow control is achieved by varying the spinning speed, spinning direction, and operations such as valve opening, siphoning, volume metering, mixing, and flow switching. In our previous studies, a LOAD platform was built for allergy testing [ 16 , 17 ]. In our second generation LOAD, the AO-loaded cells that were sensitized with a patient’s serum or medium alone were challenged with suspected allergens or other triggers in chamber at body temperature (37 °C).…”