An integrated approach to the structural characterization, long-term toxicological and anti-inflammatory evaluation of Pterospermum rubiginosum bark extract

Targeting the Role of PRME in Regulating Bone Remodelling During Postmenopausal Osteoporosis

“…The pure compounds isolated from PRME were identified as 3,7-dimethyl naphthalene-1-ol, vanillic acid, ergot-4-ene-3-one, 4-O-methyl gallic acid, beta-sitosterol, catechin, beta-sitosterol glucoside, Eresveratrol, 4'-O-methyl gallocatechin, and gallocatechin. [9] ADMET analysis showed that out of nine compounds, Eresveratrol (445154), Vanillic acid (8468), and 4-O-methyl gallic acid (78016) possess good human intestinal absorption and blood-brain barrier (BBB) penetration at 99 % confidence levels and fulfil the criteria of Lipinski drug-likeness. In contrast, the catechin (73160) and 4-O-methyl gallocatechin (10087345) showed good intestinal absorption and BBB penetration at a 95 % confidence level.…”

“…The 3-month toxicological study showed that PRME up to a concentration of 250 mg/day is non-toxic. [9] The toxicological evaluation of PRME by liver enzymes such as serum glutamic oxaloacetic transaminase (SGOT) (78.61 � 7.54 U/L) and serum glutamic pyruvic transaminase (SGPT) (30.18 � 6.69 U/L) showed no significant variation. The synthetic capacity of hepatocytes is assessed by evaluating the total protein and albumin of PRMEadministered animals, which are in the normal range (Table 2).…”

“…Pterospermum rubiginosum belongs to the family Malvaceae, used by local Kani tribal healers as "Ellooripatta" due to its excellent bone regeneration potential of bark. [8][9] Despite the numerous studies on the pharmacological potential of Pterospermum rubiginosum methanolic bark extract (PRME) in invitro models, its effects on the PMO caused by estrogen deficiency are unknown. The chosen SD rat model has been a well-accepted rodent model for investigating bone erosion damage triggered by estrogen deficiency.…”

“…Natural product derivatives are commonly practised in different local and traditional systems of medicine to restore bone density by promoting bone formation. Pterospermum rubiginosum belongs to the family Malvaceae, used by local Kani tribal healers as “Ellooripatta” due to its excellent bone regeneration potential of bark [8–9] . Despite the numerous studies on the pharmacological potential of Pterospermum rubiginosum methanolic bark extract (PRME) in invitro models, its effects on the PMO caused by estrogen deficiency are unknown.…”

In silico, anti-inflammatory and acute toxicological evaluation of an indigenous medicinal plant Pterospermum rubiginosum using Sprague-Dawley rats

“…In this study, P. rubiginosum was selected owing to its traditional use as an anti-inflammatory agent in formulations. Structural characterisation of P. rubiginosum bark extract was performed by NMR spectroscopy and revealed the presence of Vanillic acid, 4- O -Methylgallic acid, E -resveratrol, Gallocatechin, 4′- O -methylgallocatechin, and catechin [ 22 ].…”