An Insight into Vital Genes Responsible for β-cell Formation

Narayan, Gloria; R, Ronima K; Agrawal, Akriti; Thummer, Rajkumar P.

doi:10.1007/5584_2023_778

Advances in Experimental Medicine and Biology

2023

DOI: 10.1007/5584_2023_778

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An Insight into Vital Genes Responsible for β-cell Formation

Gloria Narayan

Ronima K R

Akriti Agrawal

et al.

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“…[15] Due to the pivotal roles played by PDX1 in pancreatogenesis, this factor has also been extensively used in the field of reprogramming terminally differentiated non-β-cells to β-cells or differentiation of pluripotent stem cells to β-cells. [16][17][18] However, groups have used viral-based methods to introduce and express PDX1, which hugely compromised the clinical applicability of the reprogrammed cells.…”

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confidence: 99%

Biological activity of recombinant human PDX1 protein produced from Escherichia coli

Narayan,

Sen,

Nagotu

et al. 2023

J Biochem & Molecular Tox

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Pancreatic and duodenum homeobox 1 (PDX1) is considered as a pivotal transcription factor that acts as a “master regulator” in pancreatogenesis and maintenance of β‐cells. Earlier study has reported that PDX1 also functions as a tumor suppressor in human gastric cancer cells by inhibiting cell growth. Here, we report the bioactivity of the purified human PDX1 fusion protein using various assays like cell migration, proliferation, cell cycle analysis, and gene expression. In cancer cells, recombinant PDX1 protein reduced cell migration and proliferation, and arrested cell growth by inducing apoptosis in gastric cancer cells. In pancreatic ductal cancer cells, the application of the PDX1 protein resulted in the induction of insulin gene expression. The results of these experiments demonstrate the biological activity imparted by recombinant human PDX1 fusion protein on gastric and pancreatic cancer cells and its usefulness as a biological tool to elucidate its function in various cellular processes.

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mentioning

confidence: 99%

Biological activity of recombinant human PDX1 protein produced from Escherichia coli

Narayan,

Sen,

Nagotu

et al. 2023

J Biochem & Molecular Tox

Self Cite

View full text Add to dashboard Cite

show abstract

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An Insight into Vital Genes Responsible for β-cell Formation

Cited by 1 publication

References 177 publications

Biological activity of recombinant human PDX1 protein produced from Escherichia coli

Biological activity of recombinant human PDX1 protein produced from Escherichia coli

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