An Innovative Technique for Artificial Inoculation of Rhizoctonia solani Kuhn for Field Experiments

Dutta, Pranab; Deb, Lipa

doi:10.20546/ijcmas.2020.912.129

Cited by 5 publications

(5 citation statements)

References 7 publications

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“…Mithilesh [15] conducted a similar pathogenicity test using R. solani mass multiplied on corn-sand meal. Comparatively similar results were also reported by Das et al [23] in potatoes, Yildirim and Erper [24] in vegetable crops, Basbagci et al [13] in chickpeas, Burreto et al [25] in suger beet and Dutta and Deb [14] in rice and maize.…”

Section: Molecularsupporting

confidence: 87%

“…Inoculated flasks were then kept at room temperature until the entire media was covered with white mycelial growth and the flasks were gently shaken at intervals to ensure uniform growth. Two-week-old cultures were utilized for soil inoculation [14].…”

Section: Pathogenicity Assay Of Rhizoctonia Solanimentioning

confidence: 99%

“…Observations were recorded on disease development and incidence. Infected, inoculated plants displaying characteristic rootrot symptoms were re-isolated and brought back into a pure form [15,14].…”

Section: Pathogenicity Assay Of Rhizoctonia Solanimentioning

confidence: 99%

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Morphological, Molecular Identification and Pathogenicity of Rhizoctonia solani Kuhn: A Seed and Soil Borne Pathogen Infecting French Bean

Smitha H. S.,

Prashantha A.,

Koulagi

et al. 2023

IJECC

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French bean (Phaseolus vulgaris L.), holds significant importance as a both vegetable and pulse crop in India. Root rot disease has become threat for successful french bean cultivation. Fusarium spp., Sclerotium rolfsii, Rhizoctonia sp., Macrophomina, Pythium spp. and Thielaviopsis sp. may incite root rot disease in french bean. The root rot caused by Rhizoctonia soalni Kuhn is one among the root rot causing pathogens, is a major devasticating disease in this crop. The objective of this investigation was to perform the morphological, molecular identification and pathogenicity of R. solani Kuhn infecting french bean root rot. The pathogen was isolated from the naturally infected french bean field with characteristic symptoms like yellowing, drying at the soil level, stunting, poor seedling establishment, uneven growth, chlorosis, and premature defoliation in severely affected cases. It was isolated using standard tissue isolation technique and identified based on morphological traits. The fungus was molecular identified by sequencing ITS region of rDNA by using ITS1 and ITS4 primers. Further, pathogenicity of R. solani was proved according to Koch’s postulates.

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Section: Molecularsupporting

confidence: 87%

Section: Pathogenicity Assay Of Rhizoctonia Solanimentioning

confidence: 99%

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Morphological, Molecular Identification and Pathogenicity of Rhizoctonia solani Kuhn: A Seed and Soil Borne Pathogen Infecting French Bean

Smitha H. S.,

Prashantha A.,

Koulagi

et al. 2023

IJECC

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“…The four treatments include seed treatment and soil drenching of T. afroharzianum BThr29, T. asperellum BTas25, and T. erinaceum BTer43 in challenge-inoculated pots with S. rolfsii and R. solanii. Pathogen inoculums were prepared on maize sand meal (MSM) media 77 . Fifteen days old MSM media with sufficient inoculums of the two pathogens were uniformly distributed (5 g/Kg of sterilized soil) in each pot.…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity and antagonistic properties of Trichoderma strains from the crop rhizospheres in southern Rajasthan, India

Jambhulkar,

Singh,

Raja

et al. 2024

Sci Rep

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There are fewer studies on Trichoderma diversity in agricultural fields. The rhizosphere of 16 crops was analyzed for Trichoderma species in 7 districts of Rajasthan state of India. Based on DNA sequence of translation elongation factor 1α (tef-1α), and morphological characteristics, 60 isolates were identified as 11 species: Trichoderma brevicompactum, species in Harzianum clade identified as T. afroharzianum, T. inhamatum, T. lentiforme, T. camerunense, T. asperellum, T. asperelloides, T. erinaceum, T. atroviride, T. ghanense, and T. longibrachiatum. T. brevicompactum is the most commonly occurring strain followed by T. afroharzianum. No new species were described in this study. T. lentiforme, showed its first occurrence outside the South American continent. The morphological and cultural characteristics of the major species were observed, described, and illustrated in detail. The isolates were tested for their antagonistic effect against three soilborne plant pathogens fungi: Sclerotium rolfsii, Rhizoctonia solani, and Fusarium verticillioides in plate culture assays. One of the most potent strains was T. afroharzianum BThr29 having a maximum in vitro inhibition of S. rolfsii (76.6%), R. solani (84.8%), and F. verticillioides (85.7%). The potential strain T. afroharzianum BThr29 was also found to be efficient antagonists against soil borne pathogens in in vivo experiment. Such information on crop selectivity, antagonistic properties, and geographic distribution of Trichoderma species will be beneficial for developing efficient Trichoderma-based biocontrol agents.

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“…R. solani were inoculated on the seeds in the soils by attaching 5 cork drills (1 cm in diameter). Inoculation of fungi was carried out simultaneously with planting seeds in polybags (Dutta & Deb, 2020). The infestation of P. fluorescens P60 raw secondary metabolites enriched with chitosan in a liquid formula was applied in two ways, namely by soaking the seeds for 2 hours at a dose of 20 ml and spraying directly onto plants with a density of 1.84 x 10 9 , 1.96 x 10 9 , and 2.18 x 10 9 cfu mL -1 for the concentration of 1, 2, and 3%, respectively, as much as 20 mL per crop after planting with 10-day intervals for further spraying up to 4 sprays.…”

Section: In Planta Testmentioning

confidence: 99%

Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight

2021

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Corn is a strategic cereal with economic value and always face the corn sheath blight in the field. An alternative safely and environmentally friendly disease control is the use of biological agent Pseudomonas fluorescens P60 that produced raw secondary metabolites. This study aimed to test the effectiveness of chitosan enriched P. fluorescens P60 raw secondary metabolites against leaf blight and to determine the effect on the growth of maize. The research was conducted at the Laboratory of Plant Protection and Experimental Farm, Faculty of Agriculture, Jenderal Soedirman University. In vitro test used a completely randomized design and in planta test used a randomized block design consisted of four treatments and six replicates. The treatments consisted of control and the secondary metabolites of P. fluorescens P60 enriched with chitosan 1, 2, and 3%. Variables observed were inhibition ability, chitinase and protease analysis qualitatively, incubation period, disease intensity, infection rate, AUDPC, crop height, crop fresh weight, and root fresh weight. The results showed that the secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in inhibiting the growth of Rhizoctonia solani as 63.3%, produced chitinase and protease, and could control the disease by delaying the incubation period as 79.05%, lowering the disease intensity as 68.68%, lowering the infection rate as 100%, and lowering AUDPC as 83.32%. The secondary metabolites of P. fluorescens P60 enriched with chitosan 3% was effective in improving plant growth by increasing plant fresh weight as 33.9% and root fresh weight as 43.09% compared to control. Chitosan could be used for improving effectiveness of antagonistic bacteria raw secondary metabolites. The raw secondary metabolites of bacterial antagonists could be improved their effectiveness against plant diseases by enrichment of chitosan.

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An Innovative Technique for Artificial Inoculation of Rhizoctonia solani Kuhn for Field Experiments

Cited by 5 publications

References 7 publications

Morphological, Molecular Identification and Pathogenicity of Rhizoctonia solani Kuhn: A Seed and Soil Borne Pathogen Infecting French Bean

Morphological, Molecular Identification and Pathogenicity of Rhizoctonia solani Kuhn: A Seed and Soil Borne Pathogen Infecting French Bean

Genetic diversity and antagonistic properties of Trichoderma strains from the crop rhizospheres in southern Rajasthan, India

Raw Secondary Metabolites of Chitosan-enriched Pseudomonas fluorescens P60 to Control Corn Sheath Blight

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