An innovative approach to ex vitro rooting and acclimatization of Fragaria × ananassa Duch. microshoots using а biogenic silica- and green-tea-catechin-based mechanocomposite

Ambros, Elena; Toluzakova, S. Y.; Shrainer, Lydia S.; Trofimova, Elena; Novikova, Tatyana

doi:10.1007/s11627-018-9894-1

Cited by 12 publications

(5 citation statements)

References 25 publications

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“…High quality shoot multiplication stage must complain not only for high multiplication rate but also for good size shoots that are able to develop new organs specially when transferred to Ex vitro conditions [24,25,26]. In the present research, shoots reduced >50% their length when cultured in media supplied with BAP and decreasing as BAP levels increased; however, the shortest shoots were >10 mm.…”

Section: Plant Adaptation To Ex Vitro Conditionsmentioning

confidence: 46%

“…In the present research, shoots reduced >50% their length when cultured in media supplied with BAP and decreasing as BAP levels increased; however, the shortest shoots were >10 mm. Usually, micropropagated shoots shorter than 10 mm are prone to die when transferring to Ex vitro conditions due to shortage in energetic reserve supply [27,28]. The collected data showed that when shoots were cultured in the presence of 0,5 mg L -1 long was at least 33 mm, maintaining a larger standard for plant survival after transferring to Ex vitro conditions.…”

Section: Plant Adaptation To Ex Vitro Conditionsmentioning

confidence: 99%

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Micropropagación de Gynerium sagitatum Aubl. cvs “criolla”, “costera” y “martinera”

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Abdala

et al. 2020

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Section: Plant Adaptation To Ex Vitro Conditionsmentioning

confidence: 46%

Section: Plant Adaptation To Ex Vitro Conditionsmentioning

confidence: 99%

Micropropagación de Gynerium sagitatum Aubl. cvs “criolla”, “costera” y “martinera”

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Mercado

Abdala

et al. 2020

BSAA

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“…Green tea is rich in the flavanol group of polyphenols as catechins which may constitute up to 30% of the dry weight [6]. In previous studies, we have shown the growth-regulating and adaptogenic activities of the mechanocomposite based on biogenic silica and green tea flavonoids at the stages of in vitro rooting and ex vitro acclimatization of F. × ananassa plantlets [7][8]. Since mechanocomposite contains silicon compounds and catechins with antioxidant properties [9][10], in this work, for the first time, the potential antioxidant protective effect, including superoxide dismutase, catalase, and peroxidase enzymes, of this environmentally friendly preparation will be analyzed under micropropagation of strawberry.…”

Section: Introductionmentioning

confidence: 99%

Effect of silicon chelates from plant raw materials on some physiological parameters in strawberry under in vitro conditions

2020

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For the first time, the effect mechanocomposite based on biogenic silica and green tea flavonoids (MC) on reduction of oxidative stress in microshoots of strawberry plantlets (cv. “Solnechnaya polyanka”) during in vitro multiplication, shoot elongation and rooting stages were established. Compared with the plantlets cultured in MC-free media, the ones cultured in media supplemented with MC showed increased key antioxidant enzyme activities (superoxide dismutase – SOD, peroxidase – POD, catalase – CAT). SOD activity increased from 1.1 to 1.4 times at 2.5 and 5.0 mg L-1 MC, POD activity – from 1.8 to 3.8 times at 5.0 and 10.0 mg L-1 MC, and CAT activity – from 1.1 to 1.4 times at 5.0 mg L-1 MC depending on the stage of micropropagation. The high antioxidant enzyme activities correlated with the decrease of H2O2 content (up to 1.7 times compared with the control). The revealed changes in the physiological status of strawberry plantlets in the presence of MC contribute to the further successful acclimatization of regenerants to ex vitro conditions. Thus, these results have demonstrated the potential of “green chemistry” for the production of healthy plant material of strawberry cultivars.

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“…Alterations of the phenolic profile have been associated with other considerable physiological transformations: enhanced antioxidant enzymatic activity, vegetative growth, dry matter production, and formation of photosynthetic pigments, as well as reduced hydrogen peroxide accumulation and modulation of concentrations of endogenous plant growth regulators [9,[23][24][25]. Some authors have suggested that the silicon-stimulated production of flavonoids and organic acids may be a strategy used by plants to cope with exogenous stressors [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Transformation of Strawberry Plants’ Phenolic Profile after Treatment with a Mechanocomposite Based on Silicon Chelates in the Course of Development under In Vitro, Ex Vitro, and In Vivo Conditions

et al. 2023

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The positive effect of silicon on plants is thought to be mediated by a modification of phenolic metabolism. The purpose of the study was to evaluate the effect of a silicon-based mechanocomposite (MC) on alterations of the phenolic profile of strawberry plants in the course of development under in vitro, ex vitro, and in vivo conditions. Aqueous ethanol extracts of aboveground parts of in vitro–derived plants (Fragaria × ananassa cv. ‘Solnechnaya polyanka’) were subjected to HPLC. Nineteen individual phenolic compounds (hydroxybenzoic and hydroxycinnamic acids, catechins, ellagic acid derivatives, and flavonol glycosides) were quantified. The results revealed phenolic profiles specific to each studied stage and significant transformations of the profiles by the MC. It induced strong upregulation of hydroxycinnamic acid during in vitro rooting and of catechins and hydroxybenzoic acids during ex vitro acclimation. At ex vitro and in vivo stages, the emergence of quercetin glycosides and ellagitannins was registered, and the MC elevated their levels during ex vitro acclimation and field growth. Principal component analysis confirmed the significant effect of the MC on the phenolic profile at all stages, and this effect was the strongest during ex vitro acclimation. The results are consistent with previous reports on the modification of phenolic profiles of plants by silicon-derived biostimulants.

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An innovative approach to ex vitro rooting and acclimatization of Fragaria × ananassa Duch. microshoots using а biogenic silica- and green-tea-catechin-based mechanocomposite

Cited by 12 publications

References 25 publications

Micropropagación de Gynerium sagitatum Aubl. cvs “criolla”, “costera” y “martinera”

Micropropagación de Gynerium sagitatum Aubl. cvs “criolla”, “costera” y “martinera”

Effect of silicon chelates from plant raw materials on some physiological parameters in strawberry under in vitro conditions

Transformation of Strawberry Plants’ Phenolic Profile after Treatment with a Mechanocomposite Based on Silicon Chelates in the Course of Development under In Vitro, Ex Vitro, and In Vivo Conditions

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