Micropropagación de Gynerium sagitatum Aubl. cvs “criolla”, “costera” y “martinera”

Padrón, Isidro Elizas Suárez; Mercado, Jorge Gallo; Abdala, Carlos Alfredo Pérez; Diaz, Claudia Marcela Lopez; Paula, Ariela Veloso de; Meza, Dary Mendoza

doi:10.18684/bsaa(18)60-69

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…Multiplied arrow cane shoots in a semisolid conventional medium system with 0.5 mg/L BAP averaged 3 cm long , while in the present research, shoots with the same BAP supply were three-fold larger (9 cm). Similar results were observed when comparing semisolid against doublephase cultured shoots of pineapple Ananas comosus L. (Bromeliaceae) (Scherwinski-Pereira et al, 2012) and arrow cane -G. sagittatum (López & Suárez, 2018;Suárez-Padrón et al, 2020). Increased shoot size is associated with more energy reserve accumulation, resulting in more chances of survival when transferred to ex vitro conditions and better plant performance during the initial phase of field growth and development (Almeida do Vale et al, 2019;Espinosa-Reyes et al, 2019).…”

Section: Acclimatizationsupporting

confidence: 57%

Micropropagation of arrow cane, Gynerium sagittatum (Aubl.) P. Beauv. cv. Criolla, Criolla 1, and Martinera, in a double-phase medium

Diaz¹,

Padrón²,

Álvarez

2021

CTA

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To evaluate the micropropagation response of arrow cane, Gynerium sagittatum (Aubl.), plants using a double-phase medium in the multiplication stage, explants consisting of stem sections with axillary meristems from cultivars Criolla, Criolla 1, and Martinera were established in vitro in a semisolid medium. Then, they were multiplied using a double-phase medium supplied at several Benzylaminopurine (BAP) concentrations (0.0, 0.5, 1.0, 1.5, and 2.0 mg/L), followed by rooting in a culture medium supplied at several Naphthaleneacetic acid (NAA) concentrations (0.0, 0.5, 1.0, 1.5, and 20 mg/L). Both multiplied unrooted and rooted microshoots were transferred ex vitro. Treatments were distributed with a completely randomized design; data were analyzed with an ANOVA and means separated with Tukey’s test. Explants from Criolla and Martinera cultured with 0.5 mg/L BAP resulted in higher multiplication rates. All microshoots transferred to the rooting medium rooted, although NAA significantly increased the number of roots and reduced root length. Plants from all three cultivars, in vitro rooted or unrooted transferred to ex vitro conditions, showed 100 % survival and adaptation. For Criolla and Martinera, 0.5 mg/L BAP statistically increased shoot multiplication rates and NAA increased adventitious root formation and reduced root length. Plants of all cultivars survived and adapted 100 % to ex vitro conditions.

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Section: Acclimatizationsupporting

confidence: 57%

Micropropagation of arrow cane, Gynerium sagittatum (Aubl.) P. Beauv. cv. Criolla, Criolla 1, and Martinera, in a double-phase medium

Diaz¹,

Padrón²,

Álvarez

2021

CTA

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Coverage effect on hardening of arrow cane (Gynerium sagitatum Aubl.) micropropagated plants

Suárez¹,

Vellojín²,

López³

2021

Rev. Fac. Nac. Agron. Medellín

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To evaluate the effect of plastic film coverage on ex vitro acclimatization of arrow cane (Gynerium sagitatum Aubl.), shoots of “Criolla”, Martinera” and “Costera” cultivars were in vitro micropropagated in a medium supplied with 6-Benzylaminopurine and half of them were rooted in a medium with 1-Naphtalene acetic acid. Rooted and unrooted shoots were transplanted in a shade house with fog irrigation, into plastic trays (72 clusters per tray), using peat as substrate and half of them was covered with translucent plastic film during 5 days after transplant while the other half was maintained uncovered. The experiment consisted of a three-way factorial arrangement with 12 treatments distributed with a split-plot design where tray coverage was the main plot, cultivars were the split, and rooting condition was the split-plot. Each treatment (36 clusters) was repeated three times for a total of 1296 experimental units. After 40 days in the shade house, the survival rate was calculated, and plant heigth, number of shoots and number of roots data were analyzed by ANOVA (P<0.05) and means were separated by Tukey test (P<0.05). Plant survival was complete (100%) regardless of genotype, rooting, or coverage condition. Transferring plant into uncovered trays statistically resulted in higher levels for plant height, number of shoots, and number of roots. Ex vitro adaptation of micropropagated arrow cane plants without plastic film covers increased plant growth and reduced labor.

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Micropropagación de Gynerium sagitatum Aubl. cvs “criolla”, “costera” y “martinera”

Abstract: Micropropagation of Gynerium sagitatumAubl. cvs "criolla", "costera" and"martinera"* Micropropagación de Gynerium sagitatum Aubl. cvs "criolla", "costera" y "martinera" Micropropagação do Gynerium sagitatumAubl CVS "criolla", "costera" e "martinera"

Cited by 2 publications

References 31 publications

Micropropagation of arrow cane, Gynerium sagittatum (Aubl.) P. Beauv. cv. Criolla, Criolla 1, and Martinera, in a double-phase medium

Micropropagation of arrow cane, Gynerium sagittatum (Aubl.) P. Beauv. cv. Criolla, Criolla 1, and Martinera, in a double-phase medium

Coverage effect on hardening of arrow cane (Gynerium sagitatum Aubl.) micropropagated plants

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