An initial evaluation of cytotoxicity, genotoxicity and antibacterial effectiveness of a disinfection liquid containing silver nanoparticles alone and combined with a glass-ionomer cement and dentin bonding systems

Abstract

Porenczuk A, Grzeczkowicz A, Maciejewska I, et al. An initial evaluation of cytotoxicity, genotoxicity and antibacterial effectiveness of a disinfection liquid containing silver nanoparticles alone and combined with a glass-ionomer cement and dentin bonding systems.

Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study

“…This is in accordance with other studies [14][15][16][17][18][19]. Self-etch dentin adhesives showed more cytotoxicity regarding the quantitative evaluation, which is also in line with previous studies [14,24,[26][27][28]. Contradictory, other studies found that Etch-and-Rinse adhesives were more cytotoxic [11,17,[20][21][22][23][24][25].…”

“…Contrary to the quantitative assessment, every adhesive showed a statistically significant difference to the cell control regarding the qualitative evaluation including the reactivity index. Thus, all the adhesives showed a cytotoxic effect, which is in accordance with previous studies [11,[15][16][17][18]21,27,29,32]. The ranking of the cytotoxicity of monomers [43,46,47] cannot be confirmed with significant differences by the qualitative results and the reactivity index.…”

“…Contradictory, other studies found that Etch-and-Rinse adhesives were more cytotoxic [11,17,[20][21][22][23][24][25]. However, in all of the comparative studies, other methods, materials and their combinations were used [11,[14][15][16][17][18][19][20][21][22][23][24][25][26][27][28]. It should be noted that in the clinical application of Etch-and-Rinse adhesives conditioning with 37% phosphoric acid is necessary.…”

“…In future research, the evaluation for these adhesives should be extended beyond 48 h and up to a long-term trial over 30 days, as described as longer extraction times in the ISO 10993-5 [12], like in other studies [15,21,24,27,35]. In order to further assimilate the cytotoxicity of the individual dentin adhesives, further investigation methods such as MTT or XTT Assay are planned after the screening.…”

“…The test of cytotoxicity of these individual bottles is called single applied adhesive part respectively additive analysis sequentially applied adhesive parts. In addition, the evaluation was usually only quantitative [11,15,[18][19][20][21][22][23][24][25][27][28][29][31][32][33][34][35]. A combination of quantitative and qualitative assessments, which is based on ISO 10993-5, is only rarely available in the case of dental adhesives, which refer exclusively to older adhesives like etch-and-rinse ones [3].…”