An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips

Shebl, Abdelhadi M.; Zalata, Khaled; Amin, Maha M; El-Hawary, Amira Kamal

doi:10.1186/1746-1596-6-117

Cited by 41 publications

(33 citation statements)

References 11 publications

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“…Manual tissue microarray (TMA) was assembled using a mechanical pencil tip 20,21 . Cores from the surrounding normal breast tissue were also taken as an internal control.…”

Section: Methodsmentioning

confidence: 99%

Ki-67 as a prognostic marker according to breast cancer molecular subtype

Soliman

Yussif

2016

Cancer Biology & Medicine

204

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Objective: Ki-67 plays an important function in cell division, but its exact role is still unknown. Moreover, few works regarding its overall function were published. The present study evaluated the clinical significance of Ki-67 index as a prognostic marker and predictor of recurrence in different molecular subtypes of breast cancer. The relationship of Ki-67 index with different clinicopathological factors was also analyzed. Methods: Ki-67 index was measured in 107 cases of primary breast cancer from 2010-2012. These patients were evaluated for estrogen receptor, progesterone receptor, and HER2. Ki-67 was divided according to percentage levels: < 15% and > 15%. Followup ranged from 32 months up to 6 years.Results: Approximately 44, 23, 15, and 25 cases were grouped as luminal A, luminal B, HER2 subtype, and triple-negative (TN), respectively. No luminal A patients showed Ki-67 level higher than 15%, and their recurrence was 20%. In luminal B group, Ki-67 level higher than 15% was observed in 69% of patients, and recurrence was 39%. In HER2 subtype, Ki-67 was higher than 15% in 34% of cases, and recurrence was 40%. In triple-negative cases, Ki-67 was higher than 15% in 60% of cases, and recurrence was detected in 32% of patients. Patients with Ki-67 less than 15% displayed better overall survival than those with Ki-67 higher than 15% (P = 0.01). Patients with Ki-67 higher than 15% exhibited higher incidence of metastasis and recurrence than those with Ki-67 less than 15% (P = 0.000).Conclusions: Ki-67 may be considered as a valuable biomarker in breast cancer patients.

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“…Manual tissue microarray (TMA) was assembled using a mechanical pencil tip 20,21 . Cores from the surrounding normal breast tissue were also taken as an internal control.…”

Section: Methodsmentioning

confidence: 99%

Ki-67 as a prognostic marker according to breast cancer molecular subtype

Soliman

Yussif

2016

Cancer Biology & Medicine

204

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“…However, they are quite expensive. To achieve cheap TMA, Shebl et al introduced an inexpensive mechanical pencil tip method for small paraffin tissue microarrays [13]. Although up to 1,000 different tissues can be analyzed in one TMA block, all TMAs are restricted by the thickness of paraffin embedded block because they are cylindrical cores obtained by punching the donor block.…”

Section: Introductionmentioning

confidence: 99%

An alternative high output tissue microarray technique

Shi

Hou

et al. 2013

Diagn Pathol

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BackgroundTissue microarray (TMA) is a high throughput research tool, which has greatly facilitated and accelerated in situ tissue analyses. However, its productivity has been restricted due to the confined thickness of traditional donor block. Here, we introduce an improved high output TMA method that is applicable to a broader range of tissue samples.MethodsIn this method, a 3.6 cm long and 2.7 cm wide recipient block with 88 square lattices (3 mm in width) was first prepared using several commercial instruments. A 2 mm wide and 6 mm long tissue rod was then prepared using a self-made blade-shaped knife from each paraffin embedded donor block of gastrointestinal stromal tumors. These rods were manually arrayed one by one into the corresponding lattices of the 60°C pre-softened recipient block with the guide of holes drilled with a steel needle. A 70-rod TMA was made to testify this method.ResultsThe prepared TMA had well defined array configurations, good tissue morphology and fully preserved proteins and DNA. A total of 500–1000 TMA sections could be easily obtained from a TMA block.ConclusionThis low-cost and time-saving method provides an alternative sampling tool for high output TMA.Virtual SlidesThe virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1979605867857990

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“…Tissue microarray (TMA) was assembled manually using a mechanical pencil tip. [26,27] Cores from the surrounding normal breast tissue were also taken as an internal control.…”

Section: Tissue Microarray Constructionmentioning

confidence: 99%

Immunohistochemical detection of human cytomegalovirus, Epstein-Barr virus and human papillomavirus in invasive breast carcinoma in Egyptian women: A tissue microarray study

Ahmed

Yussif

2016

JST

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Background and aim: Breast cancer is the commonest malignant tumor and a common cause of cancer death in women all over the world. Some recent studies attributed breast cancer to viral infection. This study aimed to evaluate the expression of HCMV, EBV and HPV in invasive carcinoma of the breast among the Egyptian women by immunohistochemistry and whether there is a relationship between the prognostic factors of breast carcinoma and these viruses. Patients and methods: This retrospective study included 107 selected cases of invasive breast carcinoma. Slides cut from tissue microarray prepared blocks were stained immunohistochemically for HCMV, EBV and HPV antigens. The association of such viruses with the clinicopathological features, tumor recurrence and patient death was evaluated statistically. Result: HCMV, EBV and HPV were present in 43.9%, 10.3% and 24.3% of cases respectively. HCMV was associated significantly with the tumor grade, mitotic count (P = .01), IDC, ER, PR, Her2/neu and molecular subtype (P = .032, .002, .02, .005, .003) respectively. EBV was associated with the tumor size, stage and histological type (P = . 025, .005, .009) respectively. HPV wasn't associated with any of the clinicopathological characteristics. None of these viruses was associated with the tumor recurrence or patient death. Conclusion: HCMV and EBV might be contributing factors for the development and behavioural alteration of breast carcinoma, representing potential tools for the detection of specific therapies for this cancer. Further studies on a larger number of cases using other techniques such as CISH for specific typing of the viruses especially HPV can add more information.

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An inexpensive method of small paraffin tissue microarrays using mechanical pencil tips

Cited by 41 publications

References 11 publications

Ki-67 as a prognostic marker according to breast cancer molecular subtype

Ki-67 as a prognostic marker according to breast cancer molecular subtype

An alternative high output tissue microarray technique

Immunohistochemical detection of human cytomegalovirus, Epstein-Barr virus and human papillomavirus in invasive breast carcinoma in Egyptian women: A tissue microarray study

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