An Indirect Enzyme-Linked Immunosorbent Assay (ELISA) for Measuring Antibodies in Chickens Infected with Infectious Bursal Disease Virus

Abstract

An immuno-enzyme assay for measuring infectious bursal disease antibodies in chickens is described. The test is performed rapidly after coating plates overnight with partially purified antigen prepared in cell culture. Coated plates can be stored for at last 4 months. The chromatographically purified rabbit anti-chicken immunoglobulin-G, conjugated to horseradish peroxidase, was used optimally at a dilution of 1:3,000. It could be stored for at least 10 months without a reduction in titer. The test is safe, highly reproducible, specific, and sensitive. Results can be read visually or by spectrophotometry. Antibodies could be detected as early as 4 days postinfection. Serum titers rose rapidly to high levels, ranging from 1:1,600 to 1:25,600 by one week postinfection. High titers persisted for up to one year. The results of this assay compare favorably with results obtained with the agar-gel precipitin and virus-neutralization tests.

Biochemistry and Immunology of Infectious Bursal Disease Virus

“…IBDVs share common group antigens which may be detected by agar gel precipitation, the fluorescent antibody test, and ELISA (Allan et al, 1984;Jackwood et al, 1982;Hirai et al, 1972Hirai et al, , 1974Chettle et al, 1985;Cullen & Wyeth, 1975;Wood et al, 1979;Marquardt et al, 1980). Both capsid proteins (VP2 and VP3) contain epitopes that are responsible for the IBDV group antigenicity (Becht et al, 1988).…”

Enzyme‐linked immunosorbent assay (ELISA) for detecting infectious laryngotracheitis viral antibodies in chicken serum

“…In poultry virology, this test has been shown to be of value to measure antibodies against infectious bronchitis virus (Mockett and Darbyshire, 1980;Soula and Moreau, 1981), reovirus (Slaght et al, 1978), infectious bursal disease virus (Marquardt et al, 1980;Howie and Thorsen, 1981), rotavirus (Bartz etal, 1980) and adenovirus (Dawson et al, 1980). The present paper describes the application of the ELISA to the detection of antibodies against infectious laryngotracheitis (ILT) virus in chicken serum.…”

Subclinical Infectious Bursal Disease in an Integrated Broiler Production Operation

“…In chicks vaccinated at 8 days of age, an antibody response should have been detected by 12-16 days of age using the ELISA method. 13 Instead, titers decreased until 16-20 days of age and did not show evidence of seroconversion until 2% 32 days of age, indicating that maternal Ab interfered with vaccination.…”

“…12 dures. 13 Subclinical IBD infections are diagnosed at necropsy Timing of IBD vaccinations is very important in by gross and microscopic examination of BF. Bursae obtaining active immunity in broilers with residual maternal antibodies.…”