An Increase in the Production of Butanol by Clostridium sp. Cells under the Influence of Stress Factors

Тігунова, О. О.; Bratishko, Viacheslav; Shulga, Sergiy

doi:10.3103/s009545272303009x

Cited by 3 publications

(4 citation statements)

References 31 publications

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“…A laboratory mill "Zyklon MSh 1" (Ukraine) was used for biomass grinding to a particle size of 200 mesh. An inoculation medium was used, as described earlier by Tigunova et al [8]. 5% inoculum by volume was used as an initial concentration.…”

Section: Bacterial Strains Plant Biomass and Cultivationmentioning

confidence: 99%

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Biobutanol Production Using Non-grain Biomass Sorghum saccharatum as a Substrate

Tigunova,

Rakhmetov,

Blume

et al. 2024

TOASJ

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Background The global energy challenge has recently prompted biotechnological research to explore new non-food substrates of plant origin for obtaining liquid biofuels. One of the important areas of research is the process of pretreatment and further use of non-grain biomass (lignocellulose) as a substrate for bioconversion to higher alcohols. Objective The aim of this work was to determine the macrocomponent composition and biochemical characteristics of sweet sorghum [Sorghum saccharatum (L.) Moench], select an effective bacterial culture for fermentation of the non-grain part of sorghum biomass as a substrate for obtaining biobutanol, and elaborate the best protective medium and storage temperature for lyophilization of the producer. Methods This work was conducted using butanol producing strains Clostridium sp. UCM B-7570, Clostridium acetobutylicum UCM B-7407, and C. tyrobutylicum IFBG C4B from the “Collection of Microorganism Strains and Plant Lines for Agricultural and Industrial Biotechnology” of the Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine. The bacterial cultures were cultivated on the sweet sorghum biomass provided by the National Botanical Garden named after M. M. Gryshko of the National Academy of Sciences of Ukraine. A gas chromatograph was used to determine the presence of ethanol, acetone, and butanol in the cultural liquid. Results It has been established that the proposed improvement of the biobutanol production process made it possible to obtain 8 g/dm3 of the target product from 60 g of dry green biomass of sweet sorghum of the Energodar variety. The composition of the protective medium for drying the Clostridium sp. UCM B-7570 culture and its storage period in the lyophilic form have been optimized. Conclusion The obtained results demonstrate the possibility of using the biomass of different varieties of sweet sorghum as a substrate for obtaining biobutanol, and the optimized storage conditions of the Clostridium sp. UCM B-7570 culture can minimize the possibility of its degradation.

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Section: Bacterial Strains Plant Biomass and Cultivationmentioning

confidence: 99%

“…), is the main reason affecting the economics of the ABE process [7]. These circumstances and some characteristics of saccharolytic clostridia to use different carbohydrates have stimulated research into the use of alternative substrates of plant origin [8].…”

Section: Introductionmentioning

confidence: 99%

Biobutanol Production Using Non-grain Biomass Sorghum saccharatum as a Substrate

Tigunova,

Rakhmetov,

Blume

et al. 2024

TOASJ

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show abstract

“…Cultivation of microorganisms was carried out using a standard differential enhanced clostridial medium (Condalab, Spain) according to the method (Tigunova et al, 2023) in a "Crystal" anaerostat (Germany) in a nitrogen atmosphere. Cultivation was carried out in 500 ml asks lled with 250 ml medium and hydrochloric acid modules.…”

Section: Cultivation and Solvent Determinationmentioning

confidence: 99%

“…After 72 h of cultivation, the cells were sedimented for 10 min using an ultracentrifuge "Labofuge 400R" (Germany) at a speed of 13,000 rpm, and fermentation products were collected from the culture liquid. Biomass was determined by the weight method (Tigunova et al, 2023). The Presence of ethanol, acetone, and butanol in the culture liquid was determined using a gas chromatograph (a ame ionization detector and a packed column 3 m long, phase -Сarbovax 1500 on the chromaton N-A-W-DMSC (0.20-0.25 mm).…”

Section: Cultivation and Solvent Determinationmentioning

confidence: 99%

Apple Pomace as an Alternative Substrate for Butanol Production

Tigunova,

Bratishko,

Shulga

2023

Preprint

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Butanol-producing strains Clostridium sp. UCM B-7570 and C. acetobutylicum UCM B-7407 were used for research from "Collection of strains of microorganisms and plant lines for food and agricultural biotechnology" of the Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, glycerol (BASF, Germany) and apple pomace (total moisture 4%) after apple juice production. The aim of this work was to study the possibility of using apple pomace by domestic butanol-producing strains of Clostridium sp. UCM B-7570 and C. acetobutylicum UCM B-7407 as a substrate. Producers were cultured on medium with different concentrations of apple pomace, glycerol was used for the inoculation. The presence of ethanol, acetone, and butanol in the culture liquid was determined using a gas chromatograph. It was determined that a significant part of the macrocomponent composition of the extracts can be used in bioconversion by producing strains of the genus Clostridium. It was determined that the highest concentration of butanol (10 g/dm3) was at a concentration of 120 g/dm3 in the extracts. The obtained data showed the possibility of using apple pomace as a substrate in biobutanol technology.

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Apple pomace as an alternative substrate for butanol production

Tigunova,

Bratishko,

Shulga

2023

AMB Expr

View full text Add to dashboard Cite

Butanol-producing strains Clostridium sp. UCM B-7570 and C. acetobutylicum UCM B-7407 were used for research from “Collection of strains of microorganisms and plant lines for food and agricultural biotechnology” of the Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine, glycerol (BASF, Germany) and apple pomace (total moisture 4%) after apple juice production. The aim of this work was to study the possibility of using apple pomace by domestic butanol-producing strains of Clostridium sp. UCM B-7570 and C. acetobutylicum UCM B-7407 as a substrate. Producers were cultured on medium with different concentrations of apple pomace, glycerol was used for the inoculation. The presence of ethanol, acetone, and butanol in the culture liquid was determined using a gas chromatograph. It was determined that a significant part of the macrocomponent composition of the extracts can be used in bioconversion by producing strains of the genus Clostridium. It was determined that the highest concentration of butanol (10 g/dm3) was at a concentration of 120 g/dm3 in the extracts. The obtained data showed the possibility of using apple pomace as a substrate in biobutanol technology.

show abstract

An Increase in the Production of Butanol by Clostridium sp. Cells under the Influence of Stress Factors

Cited by 3 publications

References 31 publications

Biobutanol Production Using Non-grain Biomass Sorghum saccharatum as a Substrate

Biobutanol Production Using Non-grain Biomass Sorghum saccharatum as a Substrate

Apple Pomace as an Alternative Substrate for Butanol Production

Apple pomace as an alternative substrate for butanol production

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